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- PDB-8z60: Cryo-EM structure of the polar flagellar motor-hook complex -

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Basic information

Entry
Database: PDB / ID: 8z60
TitleCryo-EM structure of the polar flagellar motor-hook complex
Components
  • (Flagellar basal-body rod protein ...) x 3
  • (Flagellar biosynthetic protein ...) x 4
  • (FlgH) x 2
  • Component of sodium-driven polar flagellar motor
  • Flagella basal-body protein
  • Flagellar M-ring protein
  • Flagellar assembly lipoprotein FlgP
  • Flagellar basal body rod protein FlgB
  • Flagellar hook protein FlgE
  • Flagellar hook-basal body complex protein FliE
  • FlgB-Dc
  • FlgI
  • FliE-helix 1
  • LRR
  • Sodium-type flagellar motor component
KeywordsMOTOR PROTEIN / Polar flagellum / Polar flagellar motor
Function / homology
Function and homology information


bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum hook / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / cytoskeletal motor activity / bacterial-type flagellum assembly / protein secretion ...bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum hook / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / cytoskeletal motor activity / bacterial-type flagellum assembly / protein secretion / bacterial-type flagellum-dependent cell motility / protein targeting / cell outer membrane / structural molecule activity / plasma membrane / cytosol
Similarity search - Function
Flagellar assembly protein T, N-terminal / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, C-terminal / FlgT, C-terminal domain superfamily / MotY N-terminal domain / Flagellar assembly protein T, C-terminal domain / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, N-terminal domain / MotY N-terminal domain / Flagellar protein FlgP ...Flagellar assembly protein T, N-terminal / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, C-terminal / FlgT, C-terminal domain superfamily / MotY N-terminal domain / Flagellar assembly protein T, C-terminal domain / Flagellar assembly protein T, middle domain / Flagellar assembly protein T, N-terminal domain / MotY N-terminal domain / Flagellar protein FlgP / : / FlgT, N-terminal domain superfamily / Flagellar basal-body rod FlgF / Flagellar biosynthetic protein FlhB / Type III secretion system substrate exporter / Type III secretion system substrate exporter, C-terminal / FlhB HrpN YscU SpaS Family / Flagellar hook-basal body complex protein FliE / Flagellar basal-body rod FlgG / Flagellar hook-basal body complex protein FliE / Flagellar basal-body rod protein FlgC / Flagellar basal-body rod protein FlgB / Flagellar biosynthesis protein FliQ / Flagellar biosynthesis protein FliR / Flagellar transport protein FliP / Sel1 repeat / Outer membrane protein, OmpA-like, conserved site / OmpA-like domain. / Sel1-like repeat / Flagellar hook protein FlgE superfamily / Sel1-like repeats. / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Bacterial export protein family 3 / Bacterial export proteins, family 3 / Type III secretion system inner membrane R protein / Bacterial export proteins, family 1 / Flagella transport protein fliP family signature 1. / Type III secretion system inner membrane P protein / FliP family / Flagella transport protein fliP family signature 2. / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Flagellar M-ring protein C-terminal / : / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / OmpA-like domain profile. / OmpA family / OmpA-like domain / OmpA-like domain superfamily / AMP-binding enzyme, C-terminal domain superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Chem-PGT / Chem-XKP / Flagellar basal body rod protein FlgB / Flagellar basal-body rod protein FlgC / Flagellar basal-body rod protein FlgF / Flagellar assembly lipoprotein FlgP / Flagellar hook-basal body complex protein FliE / Flagellar hook protein FlgE / Flagellar biosynthetic protein FliQ / Flagellar basal-body rod protein FlgG ...Chem-PGT / Chem-XKP / Flagellar basal body rod protein FlgB / Flagellar basal-body rod protein FlgC / Flagellar basal-body rod protein FlgF / Flagellar assembly lipoprotein FlgP / Flagellar hook-basal body complex protein FliE / Flagellar hook protein FlgE / Flagellar biosynthetic protein FliQ / Flagellar basal-body rod protein FlgG / Flagellar biosynthetic protein FlhB / Flagellar biosynthetic protein FliR / Flagellar biosynthetic protein FliP / Flagella basal-body protein / Component of sodium-driven polar flagellar motor / Flagellar M-ring protein / Sodium-type flagellar motor component
Similarity search - Component
Biological speciesVibrio alginolyticus (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.22 Å
AuthorsZhang, L. / Tan, J.X. / Zhou, Y. / Zhu, Y.Q.
Funding support China, 3items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81925024 China
National Natural Science Foundation of China (NSFC)U23A20163 China
Ministry of Science and Technology (MoST, China)2017YFA0503900 China
CitationJournal: Protein Cell / Year: 2026
Title: Molecular basis of high-torque transmission of the Vibrio polar flagellar motor.
Authors: Ling Zhang / Jiaxing Tan / Xuemin Duan / Xiaofei Wang / Ting Wang / Keke Yuan / Michio Homma / Seiji Kojima / Yan Zhou / Yongqun Zhu /
Abstract: The bacterial flagellar motor is a protein nanomachine that rotates the flagellum to facilitate bacterial motility. These motors exhibit structural diversity among species, enabling the transmission ...The bacterial flagellar motor is a protein nanomachine that rotates the flagellum to facilitate bacterial motility. These motors exhibit structural diversity among species, enabling the transmission of varying torques to flagellar filaments to grant bacteria diverse swimming capabilities. Compared to peritrichous flagellar motors, polar flagellar motors are faster machines that transmit higher torque to drive high-speed motility in liquids and empower swimming in viscous environments. However, structural basis of high-torque transmission of the polar flagellar motors is still unclear. Here we present a cryo-electron microscopy structure of the polar flagellar motor in complex with the hook from Vibrio alginolyticus, comprising 295 subunits from 18 proteins. Compared to the peritrichous flagellar rod, this structure reveals an increased number of inter-subunit interactions in the rod of the polar flagellar motor. Nine phospholipid molecules insert into the interface between the export apparatus and the proximal rod. The LP ring contains more electrostatic charges on the inner surface and has fewer physical contacts with the rod, while the HT ring tightly binds to the outer surface of the LP ring. FlrP, a protein of previously unknown function, is identified as a new component of the polar flagellar motor, and extensively participates in the interactions of 15 FliF peptides of the MS ring with the rod. In contrast to that in the peritrichous flagellum, the hook in the polar flagellum has two different conformational states, L- and R-types. These findings provide unprecedented insights into structural adaptations of the bacterial polar flagellar motors for high-torque transmission.
History
DepositionApr 18, 2024Deposition site: PDBJ / Processing site: PDBJ
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
0: LRR
1: Flagellar biosynthetic protein FliP
2: Flagellar biosynthetic protein FliP
3: Flagellar biosynthetic protein FliP
4: Flagellar biosynthetic protein FliQ
5: Flagellar biosynthetic protein FliQ
6: Flagellar biosynthetic protein FliQ
7: Flagellar biosynthetic protein FliQ
8: Flagellar biosynthetic protein FliR
9: LRR
A: Flagellar basal-body rod protein FlgG
A1: Flagellar hook protein FlgE
A2: Flagellar hook protein FlgE
A3: Flagellar hook protein FlgE
AA: Flagellar M-ring protein
AB: Flagellar M-ring protein
AC: Flagellar M-ring protein
AD: Flagellar M-ring protein
AE: Flagellar M-ring protein
AF: Flagellar M-ring protein
AG: Flagellar M-ring protein
AH: Flagellar M-ring protein
AI: Flagellar M-ring protein
AJ: Flagellar M-ring protein
AK: Flagellar M-ring protein
AL: Flagellar M-ring protein
AM: Flagellar M-ring protein
AN: Flagellar M-ring protein
AO: Flagellar M-ring protein
AP: Flagellar basal-body rod protein FlgG
AQ: Flagellar basal-body rod protein FlgG
AR: Flagellar basal-body rod protein FlgG
AS: Flagellar basal-body rod protein FlgG
AT: Flagellar basal-body rod protein FlgG
AU: Flagellar basal-body rod protein FlgG
AV: Flagellar basal-body rod protein FlgG
AW: Flagellar basal-body rod protein FlgG
AX: Flagellar basal-body rod protein FlgG
AY: Flagellar basal-body rod protein FlgG
AZ: Flagellar basal-body rod protein FlgG
Aa: Flagellar basal-body rod protein FlgG
Ab: Flagellar hook protein FlgE
Ac: Flagellar hook protein FlgE
Ad: Flagellar hook protein FlgE
Ae: Flagellar hook protein FlgE
Af: Flagellar hook protein FlgE
Ag: Flagellar hook protein FlgE
Ah: Flagellar hook protein FlgE
Ai: Flagellar hook protein FlgE
Aj: Flagellar hook protein FlgE
Ak: Flagellar hook protein FlgE
Al: Flagellar hook protein FlgE
Am: Flagellar hook protein FlgE
An: Flagellar hook protein FlgE
Ao: Flagellar hook protein FlgE
Ap: Flagellar hook protein FlgE
Aq: Flagellar hook protein FlgE
Ar: Flagellar hook protein FlgE
As: Flagellar hook protein FlgE
At: Flagellar hook protein FlgE
Au: Flagellar hook protein FlgE
Av: Flagellar hook protein FlgE
Aw: Flagellar hook protein FlgE
Ax: Flagellar hook protein FlgE
Ay: Flagellar hook protein FlgE
Az: Flagellar hook protein FlgE
B: Flagellar basal-body rod protein FlgG
C: Flagellar basal-body rod protein FlgG
D: Flagellar hook protein FlgE
E: Flagellar hook protein FlgE
F: Flagellar hook protein FlgE
G: Flagellar hook protein FlgE
H: Flagellar hook protein FlgE
I: Flagellar hook protein FlgE
J: Flagellar hook protein FlgE
K: Flagellar hook protein FlgE
L: Flagellar hook protein FlgE
M: Flagellar hook protein FlgE
N: Flagellar hook protein FlgE
O: Flagellar hook protein FlgE
P: Flagellar basal-body rod protein FlgG
Q: Flagellar basal-body rod protein FlgG
R: Flagellar basal-body rod protein FlgG
S: Flagellar basal-body rod protein FlgG
T: Flagellar basal-body rod protein FlgG
U: Flagellar basal-body rod protein FlgG
V: Flagellar basal-body rod protein FlgG
W: Flagellar basal-body rod protein FlgG
X: Flagellar basal-body rod protein FlgG
Y: Flagellar basal-body rod protein FlgG
Z: Flagellar basal-body rod protein FlgG
a: Flagellar basal-body rod protein FlgG
b: Flagellar basal body rod protein FlgB
c: Flagellar basal body rod protein FlgB
d: Flagellar basal body rod protein FlgB
e: Flagellar basal body rod protein FlgB
f: Flagellar basal body rod protein FlgB
g: Flagellar basal-body rod protein FlgC
h: Flagellar basal-body rod protein FlgC
i: Flagellar basal-body rod protein FlgC
j: Flagellar basal-body rod protein FlgC
k: Flagellar basal-body rod protein FlgC
l: Flagellar basal-body rod protein FlgC
m: Flagellar basal-body rod protein FlgF
n: Flagellar basal-body rod protein FlgF
o: Flagellar basal-body rod protein FlgF
p: Flagellar basal-body rod protein FlgF
q: Flagellar basal-body rod protein FlgF
r: Flagellar hook-basal body complex protein FliE
s: Flagellar hook-basal body complex protein FliE
t: Flagellar hook-basal body complex protein FliE
u: Flagellar hook-basal body complex protein FliE
v: Flagellar hook-basal body complex protein FliE
w: Flagellar hook-basal body complex protein FliE
x: Flagellar biosynthetic protein FlhB
y: Flagellar biosynthetic protein FliP
z: Flagellar biosynthetic protein FliP
B7: Flagella basal-body protein
B8: FlgH
B9: Flagellar assembly lipoprotein FlgP
B0: FlgH
CA: FlgI
CB: Flagella basal-body protein
CC: FlgH
CD: Flagellar assembly lipoprotein FlgP
CE: FlgH
CF: FlgI
CG: Component of sodium-driven polar flagellar motor
A0: Flagellar assembly lipoprotein FlgP
CH: FlgH
CI: FlgI
CJ: Flagella basal-body protein
A4: FlgH
A5: Flagellar assembly lipoprotein FlgP
A6: FlgH
A7: FlgI
A8: Flagella basal-body protein
A9: FlgH
CK: FlgH
CL: Flagellar assembly lipoprotein FlgP
CM: FlgH
CN: FlgI
CO: Flagella basal-body protein
CP: FlgH
CQ: Flagellar assembly lipoprotein FlgP
CR: FlgH
CS: FlgI
CT: Flagella basal-body protein
CU: FlgH
CV: Flagellar assembly lipoprotein FlgP
CW: FlgH
CX: FlgI
CY: Flagella basal-body protein
CZ: FlgH
Ca: Flagellar assembly lipoprotein FlgP
Cb: FlgH
Cc: FlgI
Cd: Flagella basal-body protein
Ce: FlgH
Cf: Flagellar assembly lipoprotein FlgP
Cg: FlgH
Ch: FlgI
Ci: Flagella basal-body protein
Cj: FlgH
Ck: Flagellar assembly lipoprotein FlgP
Cl: FlgH
Cm: FlgI
Cn: Flagella basal-body protein
Co: FlgH
Cp: Flagellar assembly lipoprotein FlgP
Cq: FlgH
Cr: FlgI
Cs: Flagella basal-body protein
Ct: FlgH
Cu: Flagellar assembly lipoprotein FlgP
Cv: FlgH
Cw: FlgI
Cx: Flagella basal-body protein
Cy: FlgH
Cz: Flagellar assembly lipoprotein FlgP
C1: FlgH
C2: FlgI
C3: Flagella basal-body protein
C4: FlgH
C5: Flagellar assembly lipoprotein FlgP
C6: FlgH
C7: FlgI
C8: Flagella basal-body protein
C9: FlgH
C0: Flagellar assembly lipoprotein FlgP
DA: Component of sodium-driven polar flagellar motor
B1: Flagella basal-body protein
B2: FlgH
B3: Flagellar assembly lipoprotein FlgP
B4: FlgH
B5: FlgI
B6: Flagella basal-body protein
BA: FlgH
BB: FlgI
BC: Flagella basal-body protein
BD: FlgH
BE: Flagellar assembly lipoprotein FlgP
BF: FlgH
BG: FlgI
BH: Flagella basal-body protein
BI: FlgH
BJ: Flagellar assembly lipoprotein FlgP
BK: FlgH
BL: FlgI
BM: Flagella basal-body protein
BN: FlgH
BO: Flagellar assembly lipoprotein FlgP
BP: FlgH
BQ: FlgI
BR: Flagella basal-body protein
BS: FlgH
BT: Flagellar assembly lipoprotein FlgP
BU: FlgH
BV: FlgI
BW: Flagella basal-body protein
BX: FlgH
BY: Flagellar assembly lipoprotein FlgP
BZ: FlgH
Ba: FlgI
Bb: Flagella basal-body protein
Bc: FlgH
Bd: Flagellar assembly lipoprotein FlgP
Be: FlgH
Bf: FlgI
Bg: Flagella basal-body protein
Bh: FlgH
Bi: Flagellar assembly lipoprotein FlgP
Bj: FlgH
Bk: FlgI
Bl: Flagella basal-body protein
Bm: FlgH
Bn: Flagellar assembly lipoprotein FlgP
Bo: FlgH
Bp: FlgI
Bq: Flagella basal-body protein
Br: FlgH
Bs: Flagellar assembly lipoprotein FlgP
Bt: FlgH
Bu: FlgI
Bv: Flagella basal-body protein
Bw: FlgH
Bx: Flagellar assembly lipoprotein FlgP
By: FlgH
Bz: FlgI
DB: Sodium-type flagellar motor component
DC: Sodium-type flagellar motor component
DD: Component of sodium-driven polar flagellar motor
DE: Component of sodium-driven polar flagellar motor
DF: Sodium-type flagellar motor component
DG: Sodium-type flagellar motor component
DH: Component of sodium-driven polar flagellar motor
DI: Component of sodium-driven polar flagellar motor
DJ: Sodium-type flagellar motor component
DK: Sodium-type flagellar motor component
DL: Component of sodium-driven polar flagellar motor
DM: Component of sodium-driven polar flagellar motor
DN: Sodium-type flagellar motor component
DO: Sodium-type flagellar motor component
DP: Component of sodium-driven polar flagellar motor
DQ: Component of sodium-driven polar flagellar motor
DR: Sodium-type flagellar motor component
DS: Sodium-type flagellar motor component
DT: Component of sodium-driven polar flagellar motor
DU: Component of sodium-driven polar flagellar motor
DV: Sodium-type flagellar motor component
DW: Sodium-type flagellar motor component
DX: Component of sodium-driven polar flagellar motor
DY: Component of sodium-driven polar flagellar motor
DZ: Sodium-type flagellar motor component
Da: Sodium-type flagellar motor component
Db: Component of sodium-driven polar flagellar motor
Dc: Component of sodium-driven polar flagellar motor
Dd: Sodium-type flagellar motor component
De: Sodium-type flagellar motor component
Df: Component of sodium-driven polar flagellar motor
Dg: Component of sodium-driven polar flagellar motor
Dh: Sodium-type flagellar motor component
Di: Sodium-type flagellar motor component
Dj: Component of sodium-driven polar flagellar motor
Dk: Component of sodium-driven polar flagellar motor
Dl: Sodium-type flagellar motor component
Dm: Sodium-type flagellar motor component
Dn: Component of sodium-driven polar flagellar motor
Do: Component of sodium-driven polar flagellar motor
Dp: Sodium-type flagellar motor component
Dq: Sodium-type flagellar motor component
Dr: Component of sodium-driven polar flagellar motor
Ds: Component of sodium-driven polar flagellar motor
Dt: Sodium-type flagellar motor component
Du: Sodium-type flagellar motor component
Dv: Component of sodium-driven polar flagellar motor
Dw: Component of sodium-driven polar flagellar motor
Dx: Sodium-type flagellar motor component
Dy: Sodium-type flagellar motor component
Dz: Flagellar M-ring protein
D1: Flagellar M-ring protein
D2: Flagellar M-ring protein
D3: Flagellar M-ring protein
D4: Flagellar M-ring protein
D5: Flagellar M-ring protein
D6: FlgB-Dc
D7: FlgB-Dc
D8: FlgB-Dc
D9: FlgB-Dc
D0: FlgH
EA: FliE-helix 1
EB: FliE-helix 1
EC: FliE-helix 1
ED: FliE-helix 1
EE: FliE-helix 1
EF: FliE-helix 1
EG: Flagellar M-ring protein
EH: Flagellar M-ring protein
EI: Flagellar M-ring protein
EJ: Flagellar M-ring protein
EK: Flagellar M-ring protein
EL: Flagellar M-ring protein
EM: Flagellar M-ring protein
EN: Flagellar M-ring protein
EO: Flagellar M-ring protein
EP: Flagellar M-ring protein
EQ: Flagellar M-ring protein
ER: Flagellar M-ring protein
ES: Flagellar M-ring protein
ET: Flagellar M-ring protein
EU: Flagellar M-ring protein
EV: Flagellar M-ring protein
EW: Flagellar M-ring protein
EX: Flagellar M-ring protein
EY: Flagellar M-ring protein
EZ: Flagellar M-ring protein
Ea: Flagellar M-ring protein
Eb: Flagellar M-ring protein
Ec: Flagellar M-ring protein
Ed: Flagellar M-ring protein
Ee: Flagellar M-ring protein
Ef: Flagellar M-ring protein
Eg: Flagellar M-ring protein
Eh: Flagellar M-ring protein
Ei: Flagellar M-ring protein
Ej: Flagellar M-ring protein
Ek: Flagellar M-ring protein
El: Flagellar M-ring protein
Em: Flagellar M-ring protein
En: Flagellar M-ring protein
Eo: Flagellar M-ring protein
Ep: Flagellar M-ring protein
Eq: Flagellar M-ring protein
Er: Flagellar M-ring protein
Es: Flagellar M-ring protein
Et: Flagellar M-ring protein
Eu: Flagellar M-ring protein
Ev: Flagellar M-ring protein
Ew: Flagellar M-ring protein
Ex: Flagellar M-ring protein
Ey: Flagellar M-ring protein
Ez: Flagellar M-ring protein
E1: Flagellar M-ring protein
E2: Flagellar M-ring protein
E3: Flagellar M-ring protein
E4: Flagellar M-ring protein
E5: Flagellar M-ring protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,724,288376
Polymers12,718,806367
Non-polymers5,4829
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein , 11 types, 281 molecules 09A1A2A3AbAcAdAeAfAgAhAiAjAkAlAmAnAoApAqArAsAtAuAvAwAxAyAz...

#1: Protein LRR


Mass: 21518.053 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#6: Protein ...
Flagellar hook protein FlgE


Mass: 47434.438 Da / Num. of mol.: 40 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YA78
#7: Protein ...
Flagellar M-ring protein


Mass: 63915.605 Da / Num. of mol.: 72 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: Q75N27
#8: Protein
Flagellar basal body rod protein FlgB


Mass: 14239.950 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0G9LZG6
#11: Protein
Flagellar hook-basal body complex protein FliE


Mass: 11135.679 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0Y9C1
#13: Protein ...
Flagella basal-body protein


Mass: 42417.168 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: D9N551
#14: Protein ...
FlgH


Mass: 27929.951 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#15: Protein ...
Flagellar assembly lipoprotein FlgP / Flagellar biosynthesis protein FlgP


Mass: 16303.760 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0Y943
#17: Protein ...
FlgI


Mass: 37892.234 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#18: Protein ...
Component of sodium-driven polar flagellar motor / Sodium-type flagellar protein MotY


Mass: 33528.008 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: P74944
#19: Protein ...
Sodium-type flagellar motor component / Sodium-type polar flagellar protein MotX


Mass: 24088.613 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: Q93R25

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Flagellar biosynthetic protein ... , 4 types, 11 molecules 123yz45678x

#2: Protein
Flagellar biosynthetic protein FliP


Mass: 31314.904 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A1W6TTE6
#3: Protein
Flagellar biosynthetic protein FliQ


Mass: 10329.615 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YAF4
#4: Protein Flagellar biosynthetic protein FliR


Mass: 28905.535 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YFV7
#12: Protein Flagellar biosynthetic protein FlhB


Mass: 42197.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YB55

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Flagellar basal-body rod protein ... , 3 types, 38 molecules AAPAQARASATAUAVAWAXAYAZAaBCPQRSTUVWXYZaghi...

#5: Protein ...
Flagellar basal-body rod protein FlgG / Distal rod protein


Mass: 27908.943 Da / Num. of mol.: 27 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0H0YB44
#9: Protein
Flagellar basal-body rod protein FlgC


Mass: 14812.569 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0G9LZH2
#10: Protein
Flagellar basal-body rod protein FlgF


Mass: 26987.387 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148 / References: UniProt: A0A0G9M4M9

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Protein/peptide , 3 types, 37 molecules B0CECHA6CMCRCWCbCgClCqCvC1C6B4BABFBKBPBUBZBeBjBoBtByD0D6D7D8...

#16: Protein/peptide ...
FlgH


Mass: 1450.630 Da / Num. of mol.: 27 / Fragment: The N-terminus / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#20: Protein/peptide
FlgB-Dc


Mass: 1395.589 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148
#21: Protein/peptide
FliE-helix 1


Mass: 1867.152 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Vibrio alginolyticus (bacteria) / Strain: KK148

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Non-polymers , 2 types, 9 molecules

#22: Chemical
ChemComp-PGT / (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / PHOSPHATIDYLGLYCEROL / 1-PALMITOYL-2-OLEOYL-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)](SODIUM SALT)


Mass: 751.023 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C40H79O10P / Feature type: SUBJECT OF INVESTIGATION / Comment: phospholipid*YM
#23: Chemical
ChemComp-XKP / (11R,14S)-17-amino-14-hydroxy-8,14-dioxo-9,13,15-trioxa-14lambda~5~-phosphaheptadecan-11-yl decanoate


Mass: 495.587 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C23H46NO8P / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The polar flagellar motor-hook complex / Type: COMPLEX / Entity ID: #1-#21 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Vibrio alginolyticus (bacteria) / Strain: KK148
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 1800 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)
EM imaging opticsEnergyfilter name: TFS Selectris / Energyfilter slit width: 10 eV

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
4cryoSPARCCTF correction
7UCSF ChimeraXmodel fitting
10cryoSPARCinitial Euler assignment
11cryoSPARCfinal Euler assignment
12cryoSPARCclassification
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 329955
SymmetryPoint symmetry: C100 (100 fold cyclic)
3D reconstructionResolution: 3.22 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 61395 / Symmetry type: POINT
Atomic model buildingProtocol: OTHER
Atomic model building
ID 3D fitting-IDSource nameTypeDetails (eV)
11AlphaFoldin silico model
21Otherin silico modelModelAngelo

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