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- PDB-8ywa: The structure of IgE receptor binding to IgE -

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Basic information

Entry
Database: PDB / ID: 8ywa
TitleThe structure of IgE receptor binding to IgE
Components
  • (High affinity immunoglobulin epsilon receptor subunit ...) x 3
  • (Immunoglobulin ...) x 2
KeywordsMEMBRANE PROTEIN/IMMUNE SYSTEM / immunology / Ige receptor / allergy / MEMBRANE PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


IgE receptor activity / Fc-epsilon receptor I complex / Fc receptor mediated stimulatory signaling pathway / T cell differentiation involved in immune response / high-affinity IgE receptor activity / negative regulation of mast cell apoptotic process / type I hypersensitivity / mast cell apoptotic process / Fc-gamma receptor III complex / eosinophil degranulation ...IgE receptor activity / Fc-epsilon receptor I complex / Fc receptor mediated stimulatory signaling pathway / T cell differentiation involved in immune response / high-affinity IgE receptor activity / negative regulation of mast cell apoptotic process / type I hypersensitivity / mast cell apoptotic process / Fc-gamma receptor III complex / eosinophil degranulation / serotonin secretion by platelet / positive regulation of mast cell cytokine production / neutrophil activation involved in immune response / positive regulation of mast cell degranulation / Fc-gamma receptor signaling pathway / positive regulation of type III hypersensitivity / regulation of platelet activation / Platelet Adhesion to exposed collagen / positive regulation of type IIa hypersensitivity / IgE binding / positive regulation of protein localization to cell surface / positive regulation of type I hypersensitivity / interleukin-3-mediated signaling pathway / Fc epsilon receptor (FCERI) signaling / type 2 immune response / IgG binding / phagocytosis, engulfment / mast cell degranulation / Dectin-2 family / positive regulation of interleukin-4 production / antigen processing and presentation of exogenous peptide antigen via MHC class I / Fc-epsilon receptor signaling pathway / immunoglobulin mediated immune response / tertiary granule membrane / positive regulation of interleukin-10 production / cellular response to low-density lipoprotein particle stimulus / ficolin-1-rich granule membrane / Role of LAT2/NTAL/LAB on calcium mobilization / GPVI-mediated activation cascade / neutrophil chemotaxis / FCERI mediated Ca+2 mobilization / positive regulation of phagocytosis / osteoclast differentiation / integrin-mediated signaling pathway / protein localization to plasma membrane / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCERI mediated NF-kB activation / antigen processing and presentation of exogenous peptide antigen via MHC class II / receptor internalization / positive regulation of interleukin-6 production / positive regulation of tumor necrosis factor production / cell surface receptor signaling pathway / defense response to bacterium / immune response / innate immune response / external side of plasma membrane / Neutrophil degranulation / cell surface / protein homodimerization activity / identical protein binding / plasma membrane
Similarity search - Function
High affinity immunoglobulin epsilon receptor subunit gamma / Membrane-spanning 4-domains subfamily A / CD20-like family / CD20-like family / T-cell surface glycoprotein CD3 zeta subunit/High affinity IgE receptor gamma subunit / T-cell surface glycoprotein CD3 zeta chain / : / Immunoreceptor tyrosine-based activation motif / Phosphorylated immunoreceptor signalling ITAM / ITAM motif mammalian type profile. ...High affinity immunoglobulin epsilon receptor subunit gamma / Membrane-spanning 4-domains subfamily A / CD20-like family / CD20-like family / T-cell surface glycoprotein CD3 zeta subunit/High affinity IgE receptor gamma subunit / T-cell surface glycoprotein CD3 zeta chain / : / Immunoreceptor tyrosine-based activation motif / Phosphorylated immunoreceptor signalling ITAM / ITAM motif mammalian type profile. / Immunoreceptor tyrosine-based activation motif / Immunoglobulin domain / Immunoglobulin domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
High affinity immunoglobulin epsilon receptor subunit alpha / High affinity immunoglobulin epsilon receptor subunit gamma / High affinity immunoglobulin epsilon receptor subunit beta
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.14 Å
AuthorsChen, M.Y. / Su, Q. / Shi, Y.G.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31930059 China
National Natural Science Foundation of China (NSFC)81920108015 China
CitationJournal: Nature / Year: 2025
Title: Molecular mechanism of IgE-mediated FcεRI activation.
Authors: Mengying Chen / Qiang Su / Yigong Shi /
Abstract: Allergic diseases affect more than a quarter of individuals in industrialized countries, and are a major public health concern. The high-affinity Fc receptor for immunoglobulin E (FcεRI), which is ...Allergic diseases affect more than a quarter of individuals in industrialized countries, and are a major public health concern. The high-affinity Fc receptor for immunoglobulin E (FcεRI), which is mainly present on mast cells and basophils, has a crucial role in allergic diseases. Monomeric immunoglobulin E (IgE) binding to FcεRI regulates mast cell survival, differentiation and maturation. However, the underlying molecular mechanism remains unclear. Here we demonstrate that prior to IgE binding, FcεRI exists mostly as a homodimer on human mast cell membranes. The structure of human FcεRI confirms the dimeric organization, with each promoter comprising one α subunit, one β subunit and two γ subunits. The transmembrane helices of the α subunits form a layered arrangement with those of the γ and β subunits. The dimeric interface is mediated by a four-helix bundle of the α and γ subunits at the intracellular juxtamembrane region. Cholesterol-like molecules embedded within the transmembrane domain may stabilize the dimeric assembly. Upon IgE binding, the dimeric FcεRI dissociates into two protomers, each of which binds to an IgE molecule. This process elicits transcriptional activation of Egr1, Egr3 and Ccl2 in rat basophils, which can be attenuated by inhibiting the FcεRI dimer-to-monomer transition. Collectively, our study reveals the mechanism of antigen-independent, IgE-mediated FcεRI activation.
History
DepositionMar 30, 2024Deposition site: PDBJ / Processing site: PDBC
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: Immunoglobulin heavy constant epsilon
X: Immunoglobulin heavy constant epsilon
h: Immunoglobulin kappa constant
x: Immunoglobulin kappa constant
A: High affinity immunoglobulin epsilon receptor subunit alpha
B: High affinity immunoglobulin epsilon receptor subunit beta
D: High affinity immunoglobulin epsilon receptor subunit gamma
C: High affinity immunoglobulin epsilon receptor subunit gamma
hetero molecules


Theoretical massNumber of molelcules
Total (without water)264,45917
Polymers262,4688
Non-polymers1,9919
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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High affinity immunoglobulin epsilon receptor subunit ... , 3 types, 4 molecules ABDC

#3: Protein High affinity immunoglobulin epsilon receptor subunit alpha / Fc-epsilon RI-alpha / FcERI / IgE Fc receptor subunit alpha


Mass: 30892.074 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FCER1A, FCE1A / Production host: Homo sapiens (human) / References: UniProt: P12319
#4: Protein High affinity immunoglobulin epsilon receptor subunit beta / FcERI / Fc epsilon receptor I beta-chain / IgE Fc receptor subunit beta / Membrane-spanning 4- ...FcERI / Fc epsilon receptor I beta-chain / IgE Fc receptor subunit beta / Membrane-spanning 4-domains subfamily A member 2


Mass: 30289.541 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MS4A2, APY, FCER1B, IGER / Production host: Homo sapiens (human) / References: UniProt: Q01362
#5: Protein High affinity immunoglobulin epsilon receptor subunit gamma / Fc receptor gamma-chain / FcRgamma / Fc-epsilon RI-gamma / IgE Fc receptor subunit gamma / FceRI gamma


Mass: 9676.426 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FCER1G / Production host: Homo sapiens (human) / References: UniProt: P30273

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Antibody , 2 types, 4 molecules HXhx

#1: Antibody Immunoglobulin heavy constant epsilon


Mass: 63525.254 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody Immunoglobulin kappa constant


Mass: 27441.352 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Sugars , 1 types, 9 molecules

#6: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C8H15NO6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The structure of IgE receptor binding to IgE / Type: COMPLEX / Entity ID: #3-#5, #1-#2 / Source: RECOMBINANT
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: -2000 nm / Nominal defocus min: -1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOCONTINUUM (6k x 4k)

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Processing

EM softwareName: PHENIX / Category: model refinement
CTF correctionType: NONE
3D reconstructionResolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 920752 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00410029
ELECTRON MICROSCOPYf_angle_d0.48813763
ELECTRON MICROSCOPYf_dihedral_angle_d3.9811601
ELECTRON MICROSCOPYf_chiral_restr0.0431678
ELECTRON MICROSCOPYf_plane_restr0.0041838

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