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- PDB-8yrk: Tubulin-Compound KY216: stathmin-like domain complex -

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Basic information

Entry
Database: PDB / ID: 8yrk
TitleTubulin-Compound KY216: stathmin-like domain complex
Components
  • Detyrosinated tubulin alpha-1B chain
  • Stathmin-4
  • Tubulin beta chain
  • Tubulin tyrosine ligase
KeywordsCELL CYCLE / Inhibitor
Function / homology
Function and homology information


tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Resolution of Sister Chromatid Cohesion / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Mitotic Prometaphase ...tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Resolution of Sister Chromatid Cohesion / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Mitotic Prometaphase / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / EML4 and NUDC in mitotic spindle formation / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / Separation of Sister Chromatids / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / Recruitment of NuMA to mitotic centrosomes / COPI-mediated anterograde transport / regulation of microtubule polymerization or depolymerization / microtubule-based process / positive regulation of mitotic cell cycle / post-translational protein modification / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic cell cycle / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / GTPase activity / GTP binding / Golgi apparatus / metal ion binding / cytoplasm
Similarity search - Function
: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. ...: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
: / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Tubulin beta chain / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain
Similarity search - Component
Biological speciesMus musculus (house mouse)
Gallus gallus (chicken)
Sus scrofa (pig)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.74 Å
AuthorsXia, Y.Z. / He, X.Y.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81973524 China
CitationJournal: To Be Published
Title: Tubulin-Compound KY216: stathmin-like domain complex
Authors: Xia, Y.Z. / He, X.Y.
History
DepositionMar 21, 2024Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Apr 2, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Detyrosinated tubulin alpha-1B chain
B: Tubulin beta chain
C: Detyrosinated tubulin alpha-1B chain
D: Tubulin beta chain
E: Stathmin-4
F: Tubulin tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,37124
Polymers261,3056
Non-polymers4,06618
Water59433
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)104.097, 158.982, 179.057
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Detyrosinated tubulin alpha-1B chain


Mass: 50041.273 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: Q2XVP4
#2: Protein Tubulin beta chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1UIR5
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Stmn4 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P63042
#4: Protein Tubulin tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A8V0Z8P0

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Non-polymers , 11 types, 51 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#7: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#9: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#10: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#11: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#12: Chemical ChemComp-A1D62 / (11R,16S)-11-(3,5-dichloro-4-methoxyphenyl)-4,7-dioxa-12,14-diazatetracyclo[8.7.0.03,8.012,16]heptadeca-1,3(8),9-triene-13,15-dione / Tubulin polymerization-IN-41


Mass: 435.257 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C20H16Cl2N2O5 / Feature type: SUBJECT OF INVESTIGATION
#13: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2
#14: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#15: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 33 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.84 Å3/Da / Density % sol: 56.62 %
Crystal growTemperature: 291.15 K / Method: vapor diffusion, sitting drop
Details: PEG,magnesium choride,calcium chloride,Ethylene glycol,MES

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL02U1 / Wavelength: 0.97915 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 6, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97915 Å / Relative weight: 1
ReflectionResolution: 2.74→43.54 Å / Num. obs: 75501 / % possible obs: 95.91 % / Redundancy: 10.2 % / Biso Wilson estimate: 59.78 Å2 / Rmerge(I) obs: 0.1534 / Rpim(I) all: 0.04833 / Rrim(I) all: 0.1613 / Net I/σ(I): 11.57
Reflection shellResolution: 2.74→2.838 Å / Num. unique obs: 7561 / CC1/2: 0.651

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDSdata reduction
Aimlessdata scaling
FRFSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1SA1

1sa1


Resolution: 2.74→43.54 Å / SU ML: 0.3378 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 23.6565
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2261 3714 4.92 %
Rwork0.1728 71753 -
obs0.1754 75467 95.95 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 69.79 Å2
Refinement stepCycle: LAST / Resolution: 2.74→43.54 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17352 0 250 33 17635
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003918091
X-RAY DIFFRACTIONf_angle_d0.674624561
X-RAY DIFFRACTIONf_chiral_restr0.0442670
X-RAY DIFFRACTIONf_plane_restr0.00483182
X-RAY DIFFRACTIONf_dihedral_angle_d15.91386735
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.74-2.770.3741240.27772618X-RAY DIFFRACTION97.58
2.77-2.810.31651380.25872706X-RAY DIFFRACTION97.3
2.81-2.850.28831390.24362652X-RAY DIFFRACTION97.42
2.85-2.890.30331250.26122710X-RAY DIFFRACTION97.79
2.89-2.930.35451410.26012660X-RAY DIFFRACTION97.9
2.93-2.980.27631150.24662704X-RAY DIFFRACTION98.05
2.98-3.030.33271590.22872648X-RAY DIFFRACTION97.77
3.03-3.080.30751100.21832720X-RAY DIFFRACTION97.59
3.08-3.140.27541550.20672636X-RAY DIFFRACTION97.32
3.14-3.20.25761350.21342676X-RAY DIFFRACTION97.44
3.2-3.260.29231400.2092681X-RAY DIFFRACTION96.97
3.26-3.330.25341540.21062659X-RAY DIFFRACTION97.17
3.33-3.410.26311430.20852642X-RAY DIFFRACTION96.97
3.41-3.50.26831370.19942658X-RAY DIFFRACTION96.68
3.5-3.590.24611200.17822693X-RAY DIFFRACTION96.43
3.59-3.70.20061370.16692665X-RAY DIFFRACTION96.52
3.7-3.810.22741570.16022614X-RAY DIFFRACTION95.85
3.81-3.950.21571260.15272648X-RAY DIFFRACTION95.42
3.95-4.110.19841400.15172629X-RAY DIFFRACTION95.25
4.11-4.30.1831410.14582631X-RAY DIFFRACTION94.41
4.3-4.520.1691540.13222646X-RAY DIFFRACTION95.21
4.52-4.810.17891250.13212641X-RAY DIFFRACTION94.95
4.81-5.180.18731310.14122653X-RAY DIFFRACTION94.86
5.18-5.70.21721310.16262647X-RAY DIFFRACTION94.2
5.7-6.520.23581490.17512653X-RAY DIFFRACTION93.78
6.52-8.20.20171520.15992618X-RAY DIFFRACTION92.52
8.2-43.540.19611360.14442645X-RAY DIFFRACTION88.48
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.38881616482-0.73737068607-0.5297636128212.05791967702-0.8269577694681.721255640660.00504920569653-0.03975242010590.544302802932-0.2139669468270.220682483946-0.0308371577899-0.8492956028470.244033791465-0.2213391111050.708018777889-0.1030518192770.1232107557010.409684391219-0.1420157539580.57130016103128.576245977497.4047692453.0002909412
21.19282933375-0.6722304344340.05465460681283.301988409560.4817168425192.31853755605-0.05560078579390.2873168058050.103494313859-0.6832707627130.122824748822-0.218161572472-0.5548475054470.209568093171-0.07242946668950.614266328241-0.1280316058240.09342790158810.477971537749-0.08407837786280.48400696648530.213452718883.69702710543.5430393409
32.33006964050.571217181191-0.8248860337972.887972598080.01600348696452.429111856770.03614385006440.212505313225-0.06760216782660.07699097379710.218738912451-0.42428067108-0.1118501223680.40187752892-0.2201730684730.394461626592-0.01728353448410.04653657976130.513671577326-0.1840134540430.50426641524235.03058511779.782685538855.6732573478
41.810082217610.1722828625840.3807026914342.728132293920.5039265576554.234920549780.1998592910890.06979058211410.05820464180470.304568646454-0.1812816814570.516708238427-0.163853628265-0.225733110164-0.04404336550460.391663769968-0.006286924689020.1023386697870.318004196408-0.1227028169020.54673911896217.00775114984.997942747666.7609886381
51.41021539451-0.5599700659250.1102723068643.438356889511.266939634762.18678569759-0.0325955424072-0.2818405118550.1127257617620.7097777509770.03831934695860.3264380159670.04507942589950.0465252323856-0.005455560953650.505075084155-0.04845477150010.1497716457430.407925439154-0.09473700195180.44979531728318.987641063283.891138000573.2491018558
61.656969276170.3597506543210.9979816041562.864855490612.605524128416.92372134579-0.0380270970003-0.0429455152021-0.2970105932980.7498080625610.309681074935-0.3570416656920.7415149867550.872617228099-0.2643076613830.5295354169280.0975338946943-0.01027725763790.382804871903-0.1119395791460.50467428245532.678917738562.551685497261.1879036954
71.98042074189-0.340954967545-0.3024829992983.289253620670.8532516631712.58465833490.06034634706850.1991624494620.172753272914-0.184680866974-0.09696800679080.178730033585-0.359714631311-0.1174780753450.02478514367520.3131708937690.006333414669690.01023786615390.367720878968-0.07144580522840.37307897648818.735153438160.022355442821.8692882281
82.52627896287-0.502020098029-0.01331098757212.35319399613-0.07399134509562.876732169-0.023878051117-0.03664068414920.02369946037360.01670839079870.02430447274570.400512775025-0.164617789248-0.6767842048060.008429529328720.4698391009030.04299431791580.05324622778040.614815669211-0.1642048165110.5094916614598.0926637394961.541396572740.9309444555
90.568704231048-0.1103388525230.1024071116751.259324364981.10177193742.35607418757-0.058514501347-0.1048596419450.1117597870670.383424093699-0.06461150827090.236361280680.14001022647-0.2405358548830.08632164726350.314924404435-0.05179718642520.09931206802720.444025328407-0.1152290181310.4717991025212.116218671752.429104983539.3850723314
101.86004403715-0.261576963648-0.1059541006613.387900219823.011524667047.68251328818-0.206394858349-0.110894803039-0.3358621501620.6658348775940.0420801711434-0.2367548854340.7623370165960.5839538462170.1513691831810.327330252881-0.00648599267352-0.0625797913340.2761896123270.02622586769510.4291459716525.756071443238.391253876331.0228916032
111.94522286864-1.247705562650.2305252300773.67968109476-0.2878261993491.48490812219-0.03074735989440.149087419850.145790373428-0.1191676712770.0506503951726-0.0732347945862-0.1298447023580.114583211535-0.01961993874250.273902285019-0.06606494538620.03793033293970.352036617326-0.02905657099970.34508089552220.414778062833.2671885793-11.8263547535
121.53535129334-0.931466352192-0.1126226703541.878815081810.8463989163311.496976803870.009036731131740.01053394201210.01807868951380.10255666673-0.07014885389070.1123774936870.060659867897-0.243152736160.0595895778920.256976367156-0.06924456734930.03625216108130.308287780819-0.02981717323360.3432955008497.9354914516926.03206816943.14877122047
133.85810120974-0.4128413013850.03122747143093.01966862386-0.6059558325153.13881556427-0.001959469868350.9537687100310.150428130315-0.7733526188490.2586816119960.2082362631440.0962542909984-0.332466275385-0.20432382420.603012046835-0.145270327980.03893870450470.8583076553180.01150640261080.4225142718217.51552516329.79667521784-44.6422700992
142.31866146035-0.215131578013-0.08800092124781.64771962386-0.6672623014952.08017352076-0.1422192395250.576851506046-0.265659248551-0.4039945948670.176760278178-0.2502825435040.3871964582230.0702557095105-0.02494427478260.565746077327-0.09758333554520.1020346238560.655573719449-0.2541141684340.49892383233324.5967952706-3.88056277903-37.0386702301
152.58931632821.45270112211.945473081392.93005161612-2.572323789027.81439112564-0.1121820903250.3814070291890.03881577150030.1154832152680.4522284754220.141542183257-0.376076425583-0.255817355023-0.3847206517290.365216545436-0.02012470763570.1103915174770.475581629974-0.08056674172690.40200915726218.2078054357.20696628354-23.4784890655
161.800269549210.525993434335-0.2739146448991.42130795305-0.2768906501062.82260470491-0.1492270189790.22959027379-0.257234799704-0.1377463856390.2217612200440.1981007098170.291393656468-0.482189268586-0.04318114228310.430712023808-0.1065349731180.0398211575530.442260709757-0.1060486817050.4513312924847.87372498263-3.16886107264-22.2135716508
173.48715819381-0.155605738915-2.205820216282.625596712770.7703976253014.59052778635-0.1563076658830.141241834702-0.660341869440.03260453374420.10719538832-0.3762552168070.9998886427480.640506068012-0.004749826966890.5961709161850.102111291367-0.01725497362430.531775376335-0.1769860397470.7124527044930.8287634599-16.5752775408-24.5591081212
182.83499851434-1.264274914690.235864834222.419393052510.1045374584920.2413905878560.0585479297524-0.3302425257450.2109038728570.9219146266190.0890396404232-0.5058418792260.2825155832820.195682670275-0.2108875846730.936855554931-0.0907408155604-0.02562967056290.629042903786-0.1746225161410.61426514470327.533163585593.724413706781.668571647
190.100295663135-0.040606965577-0.2070071827972.365842744822.301063966332.76143002033-0.1410574480450.0103527132513-0.004844768483320.2303372904280.605009435294-0.4068255214790.4227206489070.872947540609-0.5407009400910.4341404877380.06337553919710.05160574703340.677420464486-0.2199863184670.65541156692843.175513014528.0932148574.00554784576
202.46470027456-0.551488200657-1.940355797721.97518258227-0.1257324438783.55308580988-0.3370548342020.473559358346-0.738721207411-0.316549559840.101900556351-0.08163815443731.19184690337-0.1340799629810.2474415455740.717116857052-0.1221367881630.159434816180.507106709847-0.1055205725930.6208030850097.6691434792857.016795034474.208332631
212.790220242340.575847451488-1.083289581241.72843826442-0.4621347140463.74746840878-0.26475309454-0.687736367996-0.9704522174140.319714595582-0.076906698701-0.4953061901841.018170206690.9318830886540.2829105778320.9350089991960.24259252160.1514408002140.7823356319950.1812822189630.8478352618416.5005567238552.9419175578103.513706321
222.644867784440.418491511127-2.463161697710.601111086960.7525046326935.54396462158-0.0310932685794-0.101825909164-0.2347686108470.06930561167970.126351270407-0.09960130564660.2266179195610.41956414557-0.1120123733950.6087977619190.04676772144110.07906408581180.4432467545630.01030483544950.5869059349362.0542685002562.867274997891.3660804962
234.61624944206-0.604679556625-0.2998518668751.8874382388-1.406951812336.9452828571-0.2272150836120.603599832266-0.684706294837-0.009941216048510.3652844757610.7076268253980.755258310579-0.941851973106-0.1529783048750.572294078356-0.2466387014830.09240799194340.68539028298-0.05218563572770.749794654377-7.4535826149957.178070266479.6908911632
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 74 )AA1 - 741 - 74
22chain 'A' and (resid 75 through 110 )AA75 - 11075 - 110
33chain 'A' and (resid 111 through 199 )AA111 - 199111 - 199
44chain 'A' and (resid 200 through 311 )AA200 - 311200 - 311
55chain 'A' and (resid 312 through 401 )AA312 - 401312 - 401
66chain 'A' and (resid 402 through 437 )AA402 - 437402 - 437
77chain 'B' and (resid 2 through 236 )BF2 - 2361 - 235
88chain 'B' and (resid 237 through 336 )BF237 - 336236 - 332
99chain 'B' and (resid 337 through 391 )BF337 - 391333 - 387
1010chain 'B' and (resid 392 through 428 )BF392 - 428388 - 424
1111chain 'C' and (resid 1 through 199 )CM1 - 1991 - 199
1212chain 'C' and (resid 200 through 440 )CM200 - 440200 - 440
1313chain 'D' and (resid 2 through 86 )DR2 - 861 - 85
1414chain 'D' and (resid 87 through 236 )DR87 - 23686 - 235
1515chain 'D' and (resid 237 through 265 )DR237 - 265236 - 264
1616chain 'D' and (resid 266 through 390 )DR266 - 390265 - 380
1717chain 'D' and (resid 391 through 431 )DR391 - 431381 - 421
1818chain 'E' and (resid 6 through 46 )EV6 - 461 - 26
1919chain 'E' and (resid 47 through 143 )EV47 - 14327 - 123
2020chain 'F' and (resid 1 through 83 )FW1 - 831 - 83
2121chain 'F' and (resid 84 through 283 )FW84 - 28384 - 258
2222chain 'F' and (resid 284 through 339 )FW284 - 339259 - 314
2323chain 'F' and (resid 340 through 380 )FW340 - 380315 - 346

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