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- PDB-8xy0: Activity-stability trade-off observed in variants at position 315... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8xy0 | ||||||
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Title | Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR | ||||||
![]() | Endo-1,4-beta-xylanase A | ||||||
![]() | HYDROLASE / GH10 / Alkaliphily / Alkaline resistance / Thermostability / Xylanase | ||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nakamura, T. / Takita, T. / Mizutani, K. / Mikami, B. / Nakamura, S. / Yasukawa, K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR. Authors: Nakamura, T. / Takita, T. / Kuwata, K. / Mizutani, K. / Mikami, B. / Nakamura, S. / Yasukawa, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.8 KB | Display | ![]() |
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PDB format | ![]() | 70.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 17.9 KB | Display | |
Data in CIF | ![]() | 27.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8y1mC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 42058.156 Da / Num. of mol.: 1 / Mutation: T315Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-CA / | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.32 % / Description: thin plate |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 5.9 / Details: 0.2 M ammonium iodide, 26% w/v PEG4,000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER R 4M / Detector: PIXEL / Date: Dec 2, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 26490 / % possible obs: 94.4 % / Redundancy: 3.87 % / Biso Wilson estimate: 22.77 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.068 / Rrim(I) all: 0.078 / Net I/σ(I): 13.05 |
Reflection shell | Resolution: 1.9→2.01 Å / Redundancy: 2.46 % / Rmerge(I) obs: 0.348 / Mean I/σ(I) obs: 2.28 / Num. unique obs: 3206 / CC1/2: 0.829 / Rrim(I) all: 0.434 / % possible all: 71.3 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.54 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→44.27 Å
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Refine LS restraints |
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LS refinement shell |
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