+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8xb8 | ||||||
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タイトル | The structure of ASFV A137R | ||||||
要素 | A137R | ||||||
キーワード | VIRAL PROTEIN / dodecahedron / African swine fever virus / polymer | ||||||
機能・相同性 | A137R 機能・相同性情報 | ||||||
生物種 | African swine fever virus (アフリカ豚コレラウイルス) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | ||||||
データ登録者 | Li, C. / Song, H. / Gao, G.F. | ||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: J Virol / 年: 2024 タイトル: African swine fever virus A137R assembles into a dodecahedron cage. 著者: Changyao Li / Mingzhu Jia / Tianjiao Hao / Qi Peng / Ruchao Peng / Yan Chai / Yi Shi / Hao Song / George F Gao / 要旨: African swine fever (ASF) is a highly contagious viral disease that affects domestic and wild pigs. The causative agent of ASF is African swine fever virus (ASFV), a large double-stranded DNA virus ...African swine fever (ASF) is a highly contagious viral disease that affects domestic and wild pigs. The causative agent of ASF is African swine fever virus (ASFV), a large double-stranded DNA virus with a complex virion structure. Among the various proteins encoded by ASFV, A137R is a crucial structural protein associated with its virulence. However, the structure and molecular mechanisms underlying the functions of A137R remain largely unknown. In this study, we present the structure of A137R determined by cryogenic electron microscopy single-particle reconstruction, which reveals that A137R self-oligomerizes to form a dodecahedron-shaped cage composed of 60 polymers. The dodecahedron is literally equivalent to a 1 icosahedron where the icosahedral vertexes are located in the center of each dodecahedral facet. Within each facet, five A137R protomers are arranged in a head-to-tail orientation with a long N-terminal helix forming the edge through which adjacent facets stitch together to form the dodecahedral cage. Combining structural analysis and biochemical evidence, we demonstrate that the N-terminal domain of A137R is crucial and sufficient for mediating the assembly of the dodecahedron. These findings imply the role of A137R cage as a core component in the icosahedral ASFV virion and suggest a promising molecular scaffold for nanotechnology applications. IMPORTANCE: African swine fever (ASF) is a lethal viral disease of pigs caused by African swine fever virus (ASFV). No commercial vaccines and antiviral treatments are available for the prevention ...IMPORTANCE: African swine fever (ASF) is a lethal viral disease of pigs caused by African swine fever virus (ASFV). No commercial vaccines and antiviral treatments are available for the prevention and control of the disease. A137R is a structural protein of ASFV that is associated with its virulence. The discovery of the dodecahedron-shaped cage structure of A137R in this study is of great importance in understanding ASFV pathogenicity. This finding sheds light on the molecular mechanisms underlying the functions of A137R. Furthermore, the dodecahedral cage formed by A137R shows promise as a molecular scaffold for nanoparticle vectors. Overall, this study provides valuable insights into the structure and function of A137R, contributing to our understanding of ASFV and potentially opening up new avenues for the development of vaccines or treatments for ASF. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8xb8.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8xb8.ent.gz | 892.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8xb8.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8xb8_validation.pdf.gz | 1 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8xb8_full_validation.pdf.gz | 1 MB | 表示 | |
XML形式データ | 8xb8_validation.xml.gz | 124.4 KB | 表示 | |
CIF形式データ | 8xb8_validation.cif.gz | 206.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xb/8xb8 ftp://data.pdbj.org/pub/pdb/validation_reports/xb/8xb8 | HTTPS FTP |
-関連構造データ
関連構造データ | 38214MC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: タンパク質 | 分子量: 16114.550 Da / 分子数: 60 / 由来タイプ: 組換発現 由来: (組換発現) African swine fever virus (アフリカ豚コレラウイルス) 遺伝子: A137R CDS / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A2X0THQ0 |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: The structure of ASFV A137R / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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由来(天然) | 生物種: African swine fever virus (アフリカ豚コレラウイルス) |
由来(組換発現) | 生物種: Escherichia coli (大腸菌) |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
顕微鏡 | モデル: FEI TITAN |
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電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 60 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
-解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3次元再構成 | 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 130876 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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