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- PDB-8x4f: Solution NMR structure of a DNA hairpin formed by pure CTG repeats -
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Open data
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Basic information
Entry | Database: PDB / ID: 8x4f | ||||||||||||||||||||||||||||
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Title | Solution NMR structure of a DNA hairpin formed by pure CTG repeats | ||||||||||||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / hairpin / CTG repeat expansion / myotonic dystrophy type 1 | Function / homology | DNA / DNA (> 10) | ![]() Biological species | ![]() Method | SOLUTION NMR / simulated annealing | ![]() Guo, P. / Wan, L. / Han, D. | Funding support | 1items |
![]() ![]() Title: High-Resolution NMR Structures of Intrastrand Hairpins Formed by CTG Trinucleotide Repeats. Authors: Wan, L. / He, A. / Li, J. / Guo, P. / Han, D. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.5 KB | Display | ![]() |
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PDB format | ![]() | 40.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.9 KB | Display | ![]() |
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Full document | ![]() | 472 KB | Display | |
Data in XML | ![]() | 5.9 KB | Display | |
Data in CIF | ![]() | 7.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 4263.751 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() | ||
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#2: Chemical | ChemComp-NA / Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 0.5 mM DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*CP*TP*GP*CP*TP*GP*C)-3'), 0.02 mM DSS, 1 mM sodium phosphate, 99.96%D2O Label: 1 / Solvent system: 99.96%D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 1 mM / Label: 1 / pH: 7.0 / Pressure: 1 atm / Temperature: 288 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE NEO / Manufacturer: Bruker / Model: AVANCE NEO / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 2 | |||||||||
NMR representative | Selection criteria: fewest violations | |||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 500 / Conformers submitted total number: 5 |