PDB-8x3g: Crystal structure of metformin hydrolase from Aminobacter

ID or keywords:

Entry

Database: PDB / ID: 8x3g

Title

Crystal structure of metformin hydrolase from Aminobacter

Components

Agmatinase family protein
Arginase family protein

Keywords

HYDROLASE / Metformin hydrolase / arginase family / heterohexamer

Function / homology

: / : / :

Function and homology information

Biological species

Aminobacter sp. NyZ550 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.84 Å

Authors

Li, T. / Zhou, N.Y.

Funding support

China, 1items

Citation

Journal: Nat Commun / Year: 2024
Title: Discovery of a Ni 2+ -dependent heterohexameric metformin hydrolase.
Authors: Li, T. / Xu, Z.J. / Zhang, S.T. / Xu, J. / Pan, P. / Zhou, N.Y.

History

Deposition	Nov 13, 2023	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Sep 11, 2024	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8x3g.cif.gz	798.4 KB	Display	PDBx/mmCIF format
PDB format	pdb8x3g.ent.gz	658.1 KB	Display	PDB format
PDBx/mmJSON format	8x3g.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8x3g_validation.pdf.gz	4.2 MB	Display	wwPDB validaton report
Full document	8x3g_full_validation.pdf.gz	4.2 MB	Display
Data in XML	8x3g_validation.xml.gz	96.4 KB	Display
Data in CIF	8x3g_validation.cif.gz	134.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/x3/8x3g ftp://data.pdbj.org/pub/pdb/validation_reports/x3/8x3g	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: Agmatinase family protein

B: Agmatinase family protein

C: Arginase family protein

D: Arginase family protein

E: Arginase family protein

F: Arginase family protein

hetero molecules

237 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: electron microscopy, light scattering

Unit cell

Length a, b, c (Å)	90.430, 152.522, 173.572
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

#1: Protein	Agmatinase family protein / Metformin hydrolase alpha subunit Mass: 42224.738 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: A metformin degrader / Source: (gene. exp.) Aminobacter sp. NyZ550 (bacteria) / Strain: NyZ550 / Gene: N7E70_023840 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A9E9PQ69
#2: Protein	Arginase family protein Mass: 38024.281 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: A metformin degrader / Source: (gene. exp.) Aminobacter sp. NyZ550 (bacteria) / Strain: NYZ550 / Gene: N7E70_023835 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A9E9PPA5
#3: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C₃H₈O₃ / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: Mn / Feature type: SUBJECT OF INVESTIGATION
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1576 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.73 Å³/Da / Density % sol: 54.97 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: 0.15 M calcium chloride dihydrate, 0.1 M MES buffer system (pH 6.2), 5% (v/v) glycerol, 15% (v/v) PEG smear broad

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Data collection

Diffraction	Mean temperature: 110 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å
Detector	Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 19, 2023
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97853 Å / Relative weight: 1
Reflection	Resolution: 1.84→39.47 Å / Num. obs: 205966 / % possible obs: 99.95 % / Redundancy: 10 % / CC_1/2: 0.995 / Net I/σ(I): 12.7
Reflection shell	Resolution: 1.84→1.88 Å / Num. unique obs: 10015 / CC_1/2: 0.893

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Processing

Software

Name	Version	Classification
PHENIX	(1.20.1_4487: ???)	refinement
XDS		data reduction
XDS		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.84→39.47 Å / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 16.96 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.182	10299	5 %
R_work	0.1525	-	-
obs	0.154	205966	99.95 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 1.84→39.47 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	15355	0	16	1576	16947

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.018	15713
X-RAY DIFFRACTION	f_angle_d	1.479	21272
X-RAY DIFFRACTION	f_dihedral_angle_d	14.342	5792
X-RAY DIFFRACTION	f_chiral_restr	0.108	2287
X-RAY DIFFRACTION	f_plane_restr	0.019	2808

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.84-1.87	0.269	337	0.2237	6412	X-RAY DIFFRACTION	99
1.87-1.89	0.2594	340	0.2056	6457	X-RAY DIFFRACTION	100
1.89-1.91	0.2313	338	0.1873	6430	X-RAY DIFFRACTION	100
1.91-1.93	0.2287	340	0.1751	6462	X-RAY DIFFRACTION	100
1.93-1.96	0.2047	342	0.1598	6486	X-RAY DIFFRACTION	100
1.96-1.99	0.2005	340	0.1592	6456	X-RAY DIFFRACTION	100
1.99-2.02	0.1934	342	0.1586	6500	X-RAY DIFFRACTION	100
2.02-2.05	0.1929	338	0.1594	6427	X-RAY DIFFRACTION	100
2.05-2.08	0.1969	341	0.1533	6472	X-RAY DIFFRACTION	100
2.08-2.11	0.2158	343	0.1573	6514	X-RAY DIFFRACTION	100
2.11-2.15	0.1938	340	0.1611	6475	X-RAY DIFFRACTION	100
2.15-2.19	0.1944	340	0.1508	6454	X-RAY DIFFRACTION	100
2.19-2.23	0.1927	341	0.1547	6484	X-RAY DIFFRACTION	100
2.23-2.27	0.1913	344	0.1547	6532	X-RAY DIFFRACTION	100
2.27-2.32	0.1864	340	0.1573	6462	X-RAY DIFFRACTION	100
2.32-2.38	0.1895	342	0.1557	6500	X-RAY DIFFRACTION	100
2.38-2.44	0.1814	343	0.1598	6513	X-RAY DIFFRACTION	100
2.44-2.5	0.2013	342	0.1527	6493	X-RAY DIFFRACTION	100
2.5-2.58	0.1965	343	0.1563	6525	X-RAY DIFFRACTION	100
2.58-2.66	0.1785	344	0.1557	6534	X-RAY DIFFRACTION	100
2.66-2.76	0.1955	342	0.154	6498	X-RAY DIFFRACTION	100
2.76-2.87	0.1878	343	0.1577	6507	X-RAY DIFFRACTION	100
2.87-3	0.1785	346	0.1551	6572	X-RAY DIFFRACTION	100
3-3.15	0.1898	344	0.155	6545	X-RAY DIFFRACTION	100
3.15-3.35	0.1855	346	0.1562	6576	X-RAY DIFFRACTION	100
3.35-3.61	0.1534	345	0.142	6556	X-RAY DIFFRACTION	100
3.61-3.97	0.1582	347	0.1325	6589	X-RAY DIFFRACTION	100
3.97-4.55	0.1331	349	0.1221	6636	X-RAY DIFFRACTION	100
4.55-5.73	0.1735	352	0.1425	6684	X-RAY DIFFRACTION	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.9518	1.5261	1.0659	8.6777	4.5579	6.7541	0.1221	-0.3499	0.0613	0.7532	-0.2237	0.484	0.1096	0.0081	0.1109	0.2203	-0.0171	0.039	0.1792	0.018	0.1292	-0.2385	-25.8551	-26.5217
2	5.7639	1.6636	0.4927	2.6215	3.2016	4.4362	0.0042	-0.3351	-0.0381	0.321	-0.0271	-0.1858	0.2381	-0.0537	0.022	0.1491	0.0206	-0.0358	0.0983	0.0063	0.1164	7.1522	-29.4137	-33.2894
3	0.7062	-0.3217	0.3743	1.938	-0.6014	1.3223	0.0249	-0.1237	-0.0027	0.242	0.0237	-0.0237	0.0338	0.0108	-0.0474	0.1192	-0.005	-0.0075	0.1148	-0.0221	0.094	5.8785	-10.6903	-34.3339
4	1.4474	0.5998	-0.7014	2.835	-1.9363	6.7655	0.048	-0.1592	0.0706	0.2136	0.0187	0.1476	-0.2155	-0.1028	-0.0503	0.1027	-0.0068	0.0031	0.0918	-0.0388	0.1171	2.9019	1.1384	-34.937
5	4.1966	-2.1205	-4.8373	4.5757	5.002	8.7135	-0.005	-0.0735	0.1453	0.1746	0.0246	0.0939	0.084	0.039	-0.0113	0.1195	0.0032	-0.0174	0.0636	0.0231	0.0994	11.0623	7.1359	-31.1582
6	1.8955	-0.6782	-0.1	2.1333	0.2531	2.224	0.0142	-0.0584	0.1049	0.0734	0.0592	-0.1913	-0.0984	0.1744	-0.0668	0.1027	-0.0145	-0.0004	0.0938	-0.0181	0.1167	20.7959	9.9345	-42.4988
7	1.0703	-0.4315	-0.2544	2.7703	1.8899	3.1668	0.0479	-0.0532	-0.0394	0.1388	0.0715	-0.1873	0.0581	0.1961	-0.1262	0.1023	0.0098	-0.0166	0.1052	0.0115	0.1295	21.0203	-4.1962	-39.2009
8	3.2448	0.8346	2.8418	3.8575	3.1414	9.4247	0.0332	-0.3128	0.0407	0.5333	-0.0673	-0.1146	0.2553	-0.034	0.0306	0.1567	0.0154	-0.033	0.0985	0.0008	0.1421	18.3829	-12.1376	-29.2724
9	3.6158	1.4725	-0.2903	4.945	-1.319	3.0383	0.073	-0.2848	0.0448	0.3921	-0.077	0.2687	0.0215	-0.1483	0.0067	0.1261	0.0128	0.0166	0.1382	-0.018	0.0985	-12.712	-8.8163	-39.7292
10	1.0237	-0.4989	-0.0195	1.7952	-0.0252	0.5397	-0.0192	-0.1191	-0.05	0.2395	0.0333	0.098	0.0408	-0.0591	-0.0176	0.1216	-0.0113	0.0026	0.1216	0.0048	0.0785	-9.6006	-28.8944	-43.1134
11	4.8654	3.4332	-5.4096	4.5508	-2.136	7.4951	0.0707	-0.0592	0.0353	0.33	0.0636	0.0734	0.0605	-0.1607	-0.1149	0.1251	0.0222	0.0044	0.0867	0.0242	0.1236	-15.5262	-43.644	-46.7311
12	1.8802	-0.2509	0.103	1.4112	-0.2545	1.4558	-0.0405	0.0449	-0.0744	-0.0154	0.0434	0.0426	0.1011	-0.0493	-0.0041	0.104	-0.0065	0.0082	0.0748	-0.0045	0.0833	-9.905	-46.7026	-60.4401
13	5.6752	2.4172	-2.778	2.2717	-1.7628	3.1414	-0.0943	0.2227	-0.0451	-0.0873	0.0796	0.1	0.0505	-0.1138	0.0102	0.1145	0.0304	0.004	0.0607	-0.0183	0.1001	-13.8997	-35.4527	-60.7725
14	4.0244	2.4821	-2.2381	4.2648	-2.137	2.3668	0.0476	-0.0528	-0.0094	-0.0135	-0.048	0.0335	0.017	-0.0729	-0.0166	0.1166	0.0354	0.0041	0.0684	-0.0562	0.04	-11.216	-28.4466	-57.2535
15	5.6804	3.4	-3.7715	4.9434	-0.9367	3.4689	0.1081	0.0624	0.2298	0.1377	-0.0405	0.4066	0.0129	-0.2447	-0.0532	0.1227	0.0121	-0.0236	0.1261	0.0146	0.1016	-20.3123	-24.3915	-52.5513
16	4.9052	1.2726	-5.3693	1.2909	-0.4523	7.2112	0.038	-0.0595	0.2	0.0652	-0.1557	-0.6981	0.0596	0.6369	0.089	0.1707	0.0725	0.0243	0.2551	0.0662	0.3991	11.4188	-49.7576	-67.4644
17	3.5094	-0.6794	-0.281	7.2785	0.3235	3.8118	-0.0815	-0.0635	0.0548	-0.3497	-0.2307	-0.7498	-0.1373	0.2107	0.2535	0.1233	0.0028	0.0445	0.2558	0.0286	0.2308	16.0467	-35.9485	-78.6308
18	1.3391	0.4757	-0.8078	1.5901	-0.4619	1.4377	-0.1076	0.2293	-0.0037	-0.3837	0.1009	-0.1882	-0.0135	0.0412	-0.0034	0.2159	-0.0511	0.0441	0.2077	-0.0261	0.1362	6.6862	-31.2179	-89.3227
19	3.7723	3.7488	3.6102	6.939	6.3291	9.8468	-0.1633	0.0193	0.0863	-0.1854	0.3153	-0.3145	-0.3456	0.3257	-0.1675	0.0877	0.0309	0.0123	0.1369	0.024	0.1747	9.285	-30.1593	-78.6079
20	0.6844	2.03	-1.118	6.9324	-2.6117	3.9215	-0.1797	0.1144	-0.1161	-0.4977	0.0786	-0.2578	0.2319	-0.0151	0.1171	0.0214	0.033	0.0229	0.1665	-0.0036	0.1362	-0.1169	-48.3284	-74.3946
21	0.9278	0.1833	-0.3049	1.1067	0.3129	3.7679	-0.0676	0.2099	0.085	-0.2791	0.0142	-0.1503	-0.2452	0.2583	0.0487	0.1795	-0.0048	0.0133	0.1889	0.0511	0.1807	3.0642	1.335	-75.6369
22	5.8982	-2.9809	7.2482	3.278	-5.4116	2.018	-0.1959	-0.0458	0.4904	-0.0031	0.1867	-0.0588	-0.845	-0.1591	0.0538	0.25	-0.0023	-0.0266	0.132	0.0335	0.2477	-3.9278	14.3279	-67.2518
23	0.5559	0.1445	-0.0642	1.243	0.1233	1.0567	-0.0382	0.0795	0.1801	-0.1528	-0.0011	0.286	-0.1734	-0.2115	0.0285	0.1349	0.0423	-0.041	0.1663	0.0228	0.1957	-17.5226	-0.3795	-66.471
24	1.8066	-0.2946	0.946	2.7092	-1.2717	4.1503	-0.0277	0.282	0.0163	-0.3515	-0.0035	0.0035	0.0092	0.062	0.0414	0.1277	0.0062	0.0148	0.1391	0.0034	0.1159	-6.9751	-9.0217	-75.5843
25	4.932	-1.3025	-5.2987	2.5098	2.4969	7.0332	0.3697	0.7044	-0.0122	-0.5245	-0.3134	0.2475	-0.0376	-0.4211	-0.047	0.3022	0.1007	-0.043	0.2707	0.0575	0.2221	17.4279	12.7162	-64.8636
26	5.4778	2.2169	-0.5191	8.5783	0.0699	4.6319	0.192	0.2115	-0.287	-0.737	-0.2119	-0.0108	-0.0047	-0.2161	0.0065	0.2169	0.0962	0.0182	0.3187	-0.0124	0.1415	27.8003	-1.2539	-76.7538
27	1.1019	-0.137	1.1445	6.9232	5.5853	6.7702	0.1126	-0.0066	0.0881	-0.2633	-0.2772	0.2858	-0.2673	-0.4228	0.1691	0.1625	0.069	0.0416	0.2163	0.0133	0.1586	24.254	6.181	-61.6545
28	5.4692	0.9371	0.5945	6.1956	1.9618	3.8153	0.0323	0.6642	-0.3185	-0.7941	-0.0187	0.1908	-0.2164	-0.276	-0.0142	0.3273	0.0901	-0.0066	0.3096	0.0011	0.1748	29.5741	-0.2972	-84.559
29	2.0504	-1.7997	-0.3871	4.3616	-0.7834	3.7387	0.1696	0.1991	0.0567	-0.3072	-0.1817	-0.0465	-0.1359	-0.0201	0.0204	0.1383	0.0093	0.0293	0.2056	-0.0378	0.154	40.1724	-5.3015	-76.455
30	1.1812	-0.092	-0.3173	1.9634	0.1785	3.2535	0.0152	0.0772	-0.0879	-0.0929	0.0092	-0.345	0.1334	0.3331	-0.0289	0.1176	0.0271	0.0235	0.2101	-0.0427	0.2466	45.3004	-11.3617	-68.6928
31	0.913	-1.139	-0.5766	4.0206	1.2912	1.2397	0.017	0.0655	0.0022	-0.151	0.0302	-0.1951	-0.0347	0.0647	-0.0407	0.1148	0.0077	0.0046	0.1782	-0.0138	0.1207	31.1412	-0.0509	-59.7876
32	6.3587	-2.568	-0.3695	6.6328	-2.5971	7.4469	0.2716	0.5565	-0.3171	-0.8327	-0.2795	-0.3357	0.5413	0.1311	-0.0033	0.2322	0.048	0.0425	0.2599	-0.1029	0.3069	30.4549	-45.4815	-64.769
33	8.1917	-1.0721	-7.3642	0.8908	1.8604	7.6752	-0.0339	0.3526	-0.286	-0.1228	-0.0042	-0.0431	-0.0047	-0.2571	0.0164	0.1598	0.0339	-0.051	0.1235	-0.0416	0.1964	21.6662	-35.9995	-56.2841
34	4.0824	4.6471	-1.2812	5.3135	-1.576	0.6466	-0.151	0.1064	-0.2943	-0.3072	0.0896	-0.4584	0.0933	0.1069	0.0652	0.182	0.0629	0.0223	0.2286	-0.0283	0.2634	32.6302	-36.3463	-61.0155
35	2.0208	-6.4282	-2.6437	4.5258	0.9877	2.9199	-0.4507	-0.2066	-0.8257	0.1213	0.1797	-0.0328	0.5703	0.1641	0.2154	0.2289	0.0433	-0.0445	0.1676	-0.0438	0.3896	23.297	-51.316	-49.8349
36	1.0985	0.0078	-0.3705	1.0298	0.1384	0.5912	0.0239	-0.1862	-0.1984	0.2553	0.0032	-0.3966	0.1535	0.2086	-0.016	0.216	0.068	-0.1002	0.2166	0.0163	0.2892	29.1637	-36.3864	-37.6673
37	2.124	-2.0787	-0.3726	4.79	-0.5599	1.9412	-0.0317	-0.1141	0.4186	0.006	0.0592	-0.7675	0.0062	0.2507	-0.0267	0.1422	0.0129	-0.0258	0.1595	-0.0408	0.2124	32.2715	-23.864	-47.159
38	7.0492	-3.6229	-0.405	3.8525	-0.0831	1.4805	0.1247	0.2614	-0.0332	-0.1617	-0.1152	-0.3962	0.002	0.1733	-0.012	0.1355	0.0168	-0.013	0.1392	-0.0474	0.182	29.8015	-30.3788	-53.9625

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 8 through 27 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 28 through 46 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 47 through 129 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 130 through 159 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 160 through 182 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 183 through 252 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 253 through 329 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 330 through 357 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 8 through 46 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 47 through 159 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 160 through 182 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 183 through 252 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 253 through 297 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 298 through 329 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 330 through 357 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 6 through 39 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 40 through 80 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 81 through 291 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 292 through 310 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 311 through 345 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 25 through 108 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 109 through 126 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 127 through 259 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 260 through 335 )
25	X-RAY DIFFRACTION	25	chain 'E' and (resid 6 through 39 )
26	X-RAY DIFFRACTION	26	chain 'E' and (resid 40 through 69 )
27	X-RAY DIFFRACTION	27	chain 'E' and (resid 70 through 95 )
28	X-RAY DIFFRACTION	28	chain 'E' and (resid 96 through 126 )
29	X-RAY DIFFRACTION	29	chain 'E' and (resid 127 through 172 )
30	X-RAY DIFFRACTION	30	chain 'E' and (resid 173 through 259 )
31	X-RAY DIFFRACTION	31	chain 'E' and (resid 260 through 344 )
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 25 through 46 )
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 47 through 80 )
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 81 through 108 )
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 109 through 126 )
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 127 through 259 )
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 260 through 291 )
38	X-RAY DIFFRACTION	38	chain 'F' and (resid 292 through 329 )

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