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Open data
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Basic information
Entry | Database: PDB / ID: 8x0j | ||||||
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Title | old yellow enzymes-Q102A/Y169F/R215A/R308A | ||||||
![]() | NADPH dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / Ene reductase | ||||||
Function / homology | ![]() NADPH dehydrogenase / : / NADPH dehydrogenase activity / response to toxic substance / FMN binding / NADP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lei, W. / Wei, S. | ||||||
Funding support | 1items
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![]() | ![]() Title: Unlocking the function promiscuity of old yellow enzyme to catalyze asymmetric Morita-Baylis-Hillman reaction Authors: Lei, W. / Wei, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 139.6 KB | Display | ![]() |
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PDB format | ![]() | 108.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.9 KB | Display | ![]() |
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Full document | ![]() | 451.6 KB | Display | |
Data in XML | ![]() | 23.3 KB | Display | |
Data in CIF | ![]() | 31.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 37480.543 Da / Num. of mol.: 2 / Mutation: Q102A,Y169F,R215A,R308A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: namA, GK2332 / Production host: ![]() ![]() #2: Chemical | ChemComp-PO4 / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.6 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, hanging drop / Details: glycine,PEG 2000,glycerinum |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SEALED TUBE / Type: BRUKER D8 QUEST / Wavelength: 1.54178 Å |
Detector | Type: Bruker PHOTON II / Detector: PIXEL / Date: Jul 11, 2023 / Details: MULTILAYER |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 3.11→45.74 Å / Num. obs: 17476 / % possible obs: 98.9 % / Redundancy: 3.5 % / CC1/2: 0.967 / Rmerge(I) obs: 0.175 / Net I/σ(I): 5.8 |
Reflection shell | Resolution: 3.11→3.32 Å / Rmerge(I) obs: 0.494 / Num. unique obs: 3128 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.835 Å2
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Refinement step | Cycle: 1 / Resolution: 3.11→45.74 Å
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Refine LS restraints |
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