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Open data
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Basic information
Entry | Database: PDB / ID: 8wvf | ||||||
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Title | Crystal structure of Lsd18 mutant T189M and S195M | ||||||
![]() | Putative epoxidase LasC | ||||||
![]() | FLAVOPROTEIN / FAD dependent monooxygenase / epoxidase / mutant | ||||||
Function / homology | Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor / FAD-binding domain / FAD binding domain / antibiotic biosynthetic process / monooxygenase activity / FAD binding / FAD/NAD(P)-binding domain superfamily / FLAVIN-ADENINE DINUCLEOTIDE / Putative epoxidase LasC![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, N. / Xiao, H.L. / Chen, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Simultaneous Improvement in the Thermostability and Catalytic Activity of Epoxidase Lsd18 for the Synthesis of Lasalocid A. Authors: Liu, N. / Xiao, H. / Zang, Y. / Zhou, L. / Mencius, J. / Yang, Z. / Quan, S. / Chen, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 337.5 KB | Display | ![]() |
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PDB format | ![]() | 268.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 913.2 KB | Display | ![]() |
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Full document | ![]() | 923.7 KB | Display | |
Data in XML | ![]() | 34.1 KB | Display | |
Data in CIF | ![]() | 45.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8wvbC ![]() 8up4 S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 52720.906 Da / Num. of mol.: 2 / Mutation: T189M,S195M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.46 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / Details: 0.2 M Li2SO4, 0.1M Tris, pH 7.5, 30% PEG4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 2M / Detector: PIXEL / Date: Oct 3, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.757→20.044 Å / Num. obs: 7514 / % possible obs: 87.95 % / Redundancy: 1 % / CC1/2: 0.87 / Rmerge(I) obs: 0.268 / Rpim(I) all: 0.165 / Rrim(I) all: 0.317 / Net I/σ(I): 3.7 |
Reflection shell | Resolution: 3.757→3.897 Å / Rmerge(I) obs: 0.389 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 689 / CC1/2: 0.805 / Rpim(I) all: 0.266 / Rrim(I) all: 0.473 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 8UP4 ![]() 8up4 Resolution: 3.757→20.044 Å / SU ML: 0.51 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 32.49 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.757→20.044 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 14.6083 Å / Origin y: 9.313 Å / Origin z: 33.4028 Å
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Refinement TLS group | Selection details: all |