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- PDB-8wu7: Structure of a cis-Geranylfarnesyl Diphosphate Synthase from Stre... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8wu7 | ||||||
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Title | Structure of a cis-Geranylfarnesyl Diphosphate Synthase from Streptomyces clavuligerus | ||||||
![]() | Isoprenyl transferase | ||||||
![]() | BIOSYNTHETIC PROTEIN / cis-prenyltransferase | ||||||
Function / homology | Di-trans-poly-cis-decaprenylcistransferase-like, conserved site / Undecaprenyl pyrophosphate synthase family signature. / Decaprenyl diphosphate synthase-like / Putative undecaprenyl diphosphate synthase / Decaprenyl diphosphate synthase-like superfamily / Transferases; Transferring alkyl or aryl groups, other than methyl groups / prenyltransferase activity / magnesium ion binding / Isoprenyl transferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, F.R. / Wang, Q.L. / Pan, X.M. / Dong, L.B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Discovery, Structure, and Engineering of a cis-Geranylfarnesyl Diphosphate Synthase. Authors: Li, F.R. / Wang, Q. / Pan, X. / Xu, H.M. / Dong, L.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.4 KB | Display | ![]() |
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PDB format | ![]() | 40.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.1 KB | Display | ![]() |
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Full document | ![]() | 428.4 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 16.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8wu6C ![]() 8ylzC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32477.699 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.63 Å3/Da / Density % sol: 27.3 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, sitting drop / pH: 5.7 Details: 18% (w/v) Polyethylene glycol 4000, 0.1 M MES monohydrate (pH 5.7) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 14, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9788 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→84.37 Å / Num. obs: 13544 / % possible obs: 99.6 % / Redundancy: 10.1 % / CC1/2: 0.998 / Rmerge(I) obs: 0.035 / Net I/σ(I): 51.2 |
Reflection shell | Resolution: 2.09→2.15 Å / Rmerge(I) obs: 0.059 / Mean I/σ(I) obs: 38.1 / Num. unique obs: 1033 / CC1/2: 0.998 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.09→30.59 Å
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Refine LS restraints |
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LS refinement shell |
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