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Open data
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Basic information
Entry | Database: PDB / ID: 8wt5 | ||||||
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Title | Vaccinia Virus J5 ectodomain | ||||||
![]() | Protein J5 | ||||||
![]() | VIRAL PROTEIN / Entry Fusion Complex Protein | ||||||
Function / homology | Pox virus entry-fusion-complex G9/A16 / Pox virus entry-fusion-complex G9/A16 / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / virion membrane / membrane / Protein J5![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing / molecular dynamics | ||||||
![]() | Carillo, K.J. / Tzou, D.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Solution NMR Structure of Vaccinia Virus Protein J5 (2 - 68 a.a) Authors: Carillo, K.J. / Tzou, D.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 212.8 KB | Display | ![]() |
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PDB format | ![]() | 181.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein | Mass: 7792.966 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 0.5 mM [U-100% 13C; U-100% 15N] Vaccinia Virus Protein J5, 137 mM no label sodium chloride, 2.7 mM no label potassium chloride, 10 mM no label disodium phosphate, 1.8 mM no label potassium ...Contents: 0.5 mM [U-100% 13C; U-100% 15N] Vaccinia Virus Protein J5, 137 mM no label sodium chloride, 2.7 mM no label potassium chloride, 10 mM no label disodium phosphate, 1.8 mM no label potassium dihydrogen phosphate, 90% H2O/10% D2O Details: Sample was prepared with 13C and 15N label in 1X PBS buffer pH 6.5 Label: 13C_15N_J5 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Details: J5 protein sample was expressed with 15N and 13C label Ionic strength: 8.1 mM / Label: 13C_15N_labeled_J5 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker BRUKER AVANCE III 600 / Manufacturer: Bruker / Model: BRUKER AVANCE III 600 / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |