+Open data
-Basic information
Entry | Database: PDB / ID: 8wqn | ||||||||||||||||||
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Title | Structure of Saccharolobus solfataricus SegC (SSO0033) protein | ||||||||||||||||||
Components | SegC | ||||||||||||||||||
Keywords | UNKNOWN FUNCTION / DNA BINDING PROTEIN / PARTITION PROTEIN | ||||||||||||||||||
Function / homology | Uncharacterized protein Function and homology information | ||||||||||||||||||
Biological species | Saccharolobus solfataricus P2 (archaea) | ||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||||||||||||||
Authors | Yen, C.Y. / Lin, M.G. / Sun, Y.J. / Hsiao, C.D. | ||||||||||||||||||
Funding support | Taiwan, 5items
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Citation | Journal: Nucleic Acids Res. / Year: 2024 Title: Unraveling the structure and function of a novel SegC protein interacting with the SegAB chromosome segregation complex in Archaea. Authors: Lin, M.G. / Yen, C.Y. / Shen, Y.Y. / Huang, Y.S. / Ng, I.W. / Barilla, D. / Sun, Y.J. / Hsiao, C.D. | ||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wqn.cif.gz | 48.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wqn.ent.gz | 31.8 KB | Display | PDB format |
PDBx/mmJSON format | 8wqn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8wqn_validation.pdf.gz | 423.2 KB | Display | wwPDB validaton report |
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Full document | 8wqn_full_validation.pdf.gz | 424.4 KB | Display | |
Data in XML | 8wqn_validation.xml.gz | 9 KB | Display | |
Data in CIF | 8wqn_validation.cif.gz | 12.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wq/8wqn ftp://data.pdbj.org/pub/pdb/validation_reports/wq/8wqn | HTTPS FTP |
-Related structure data
Related structure data | 8wq8C 8yk9C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19895.371 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: C-terminal missing Source: (gene. exp.) Saccharolobus solfataricus P2 (archaea) Gene: SSO0033 / Production host: Escherichia coli (E. coli) / References: UniProt: Q981B4 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.72 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: Sodium acetate pH 4.6, calcium chloride, and MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 05A / Wavelength: 0.99984 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Aug 17, 2022 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.99984 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.8→30 Å / Num. obs: 15953 / % possible obs: 96.3 % / Redundancy: 4.1 % / CC1/2: 0.992 / CC star: 0.998 / Rmerge(I) obs: 0.072 / Rpim(I) all: 0.038 / Rrim(I) all: 0.082 / Χ2: 0.909 / Net I/σ(I): 10.6 / Num. measured all: 66122 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→28.1 Å / SU ML: 0.14 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 19.85 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→28.1 Å
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Refine LS restraints |
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LS refinement shell |
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