+Open data
-Basic information
Entry | Database: PDB / ID: 8wq8 | ||||||||||||||||||
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Title | Structure of Saccharolobus solfataricus SegC (SSO0033) protein | ||||||||||||||||||
Components | SegC | ||||||||||||||||||
Keywords | UNKNOWN FUNCTION / DNA BINDING PROTEIN / PARTITION PROTEIN | ||||||||||||||||||
Function / homology | Uncharacterized protein Function and homology information | ||||||||||||||||||
Biological species | Saccharolobus solfataricus P2 (archaea) | ||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||||||||||||||
Authors | Yen, C.Y. / Lin, M.G. / Sun, Y.J. / Hsiao, C.D. | ||||||||||||||||||
Funding support | Taiwan, 5items
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Citation | Journal: Nucleic Acids Res. / Year: 2024 Title: Unraveling the structure and function of a novel SegC protein interacting with the SegAB chromosome segregation complex in Archaea. Authors: Lin, M.G. / Yen, C.Y. / Shen, Y.Y. / Huang, Y.S. / Ng, I.W. / Barilla, D. / Sun, Y.J. / Hsiao, C.D. | ||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wq8.cif.gz | 142.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wq8.ent.gz | 112.8 KB | Display | PDB format |
PDBx/mmJSON format | 8wq8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8wq8_validation.pdf.gz | 457.6 KB | Display | wwPDB validaton report |
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Full document | 8wq8_full_validation.pdf.gz | 464.1 KB | Display | |
Data in XML | 8wq8_validation.xml.gz | 25.8 KB | Display | |
Data in CIF | 8wq8_validation.cif.gz | 36 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wq/8wq8 ftp://data.pdbj.org/pub/pdb/validation_reports/wq/8wq8 | HTTPS FTP |
-Related structure data
Related structure data | 8wqnC 8yk9C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 19895.371 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharolobus solfataricus P2 (archaea) Gene: SSO0033 / Production host: Escherichia coli (E. coli) / References: UniProt: Q981B4 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.91 Å3/Da / Density % sol: 74.96 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / Details: Tris-HCl pH 8.5 and potassium thiocyanate. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSRRC / Beamline: TPS 07A / Wavelength: 0.97625 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: May 11, 2023 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.8→30 Å / Num. obs: 39646 / % possible obs: 99.9 % / Redundancy: 6.4 % / CC1/2: 0.988 / CC star: 0.997 / Rmerge(I) obs: 0.103 / Rpim(I) all: 0.044 / Rrim(I) all: 0.112 / Χ2: 0.92 / Net I/σ(I): 8.7 / Num. measured all: 253331 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→29.43 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.33 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→29.43 Å
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Refine LS restraints |
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LS refinement shell |
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