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- PDB-8wq4: Structural basis of translation inhibition by a valine tRNA-deriv... -

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Basic information

Entry
Database: PDB / ID: 8wq4
TitleStructural basis of translation inhibition by a valine tRNA-derived fragment
Components
  • (30S ribosomal protein ...) x 24
  • 50S ribosomal protein L7Ae
  • RNA (1328-MER)
  • RNA (26-MER)
  • Small ribosomal subunit protein uS8
KeywordsRIBOSOME / Sulfolobus acidocaldarius ribosome
Function / homology
Function and homology information


ribonuclease P activity / tRNA 5'-leader removal / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / positive regulation of apoptotic signaling pathway / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit ...ribonuclease P activity / tRNA 5'-leader removal / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / positive regulation of apoptotic signaling pathway / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / zinc ion binding / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein S27ae / : / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal / Ribosomal protein S4, archaeal / Ribosomal protein S12, archaea / Ribosomal protein S3, archaeal / 30S ribosomal protein S3Ae ...Ribosomal protein S27ae / : / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal / Ribosomal protein S4, archaeal / Ribosomal protein S12, archaea / Ribosomal protein S3, archaeal / 30S ribosomal protein S3Ae / Ribosomal protein S7, archaeal / Ribosomal protein S19e, archaeal / Ribosomal protein S11, archaeal / Ribosomal protein S2, archaeal / Ribosomal protein S14, type Z, archaeal / Ribosomal protein S8e, archaeal / Ribosomal protein L7Ae, archaea / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S27 / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family / Ribosomal protein S28e signature. / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein L7Ae conserved site / Ribosomal protein L7Ae signature. / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein S14/S29 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / Ribosomal protein S2 signature 2. / K Homology domain / K homology RNA-binding domain / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / Ribosomal protein S10, conserved site / Ribosomal protein S10 signature. / Ribosomal protein S14, conserved site / Ribosomal protein S14 signature. / Ribosomal protein S2 signature 1. / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / : / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19
Similarity search - Domain/homology
UNKNOWN / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS5 ...UNKNOWN / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL8 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS31 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS17
Similarity search - Component
Biological speciesSulfolobus acidocaldarius DSM 639 (acidophilic)
Haloferax volcanii (archaea)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.53 Å
AuthorsWang, Y.H. / Zhou, J.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31971226 China
CitationJournal: Life Sci Alliance / Year: 2024
Title: Structural basis of translation inhibition by a valine tRNA-derived fragment.
Authors: Yun Wu / Meng-Ting Ni / Ying-Hui Wang / Chen Wang / Hai Hou / Xing Zhang / Jie Zhou /
Abstract: Translational regulation by non-coding RNAs is a mechanism commonly used by cells to fine-tune gene expression. A fragment derived from an archaeal valine tRNA (Val-tRF) has been previously ...Translational regulation by non-coding RNAs is a mechanism commonly used by cells to fine-tune gene expression. A fragment derived from an archaeal valine tRNA (Val-tRF) has been previously identified to bind the small subunit of the ribosome and inhibit translation in Here, we present three cryo-electron microscopy structures of Val-tRF bound to the small subunit of ribosomes at resolutions between 4.02 and 4.53 Å. Within these complexes, Val-tRF was observed to bind to conserved RNA-interacting sites, including the ribosomal decoding center. The binding of Val-tRF destabilizes helices h24, h44, and h45 and the anti-Shine-Dalgarno sequence of 16S rRNA. The binding position of this molecule partially overlaps with the translation initiation factor aIF1A and occludes the mRNA P-site codon. Moreover, we found that the binding of Val-tRF is associated with steric hindrance of the H69 base of 23S rRNA in the large ribosome subunit, thereby preventing 70S assembly. Our data exemplify how tRNA-derived fragments bind to ribosomes and provide new insights into the mechanisms underlying translation inhibition by Val-tRFs.
History
DepositionOct 11, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Apr 10, 2024Provider: repository / Type: Initial release
Revision 1.1May 22, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A16S: RNA (1328-MER)
VTRF: RNA (26-MER)
AS2P: 30S ribosomal protein S2
AS4E: 30S ribosomal protein S4e
AS4P: 30S ribosomal protein S4
AS5P: 30S ribosomal protein S5
AS6E: 30S ribosomal protein S6e
AS8E: 30S ribosomal protein S8e
S11P: 30S ribosomal protein S11
S12P: 30S ribosomal protein S12
S15P: 30S ribosomal protein S15
S17P: 30S ribosomal protein S17
S24E: 30S ribosomal protein S24e
S27E: 30S ribosomal protein S27e
S3AE: 30S ribosomal protein S3Ae
AS3P: 30S ribosomal protein S3
AS7P: 30S ribosomal protein S7
AS9P: 30S ribosomal protein S9
S10P: 30S ribosomal protein S10
S13P: 30S ribosomal protein S13
S14P: 30S ribosomal protein S14 type Z
S17E: 30S ribosomal protein S17e
S19E: 30S ribosomal protein S19e
S19P: 30S ribosomal protein S19
S27A: 30S ribosomal protein S27ae
S28E: 30S ribosomal protein S28e
SL7A: 50S ribosomal protein L7Ae
AS8P: Small ribosomal subunit protein uS8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)892,48885
Polymers886,61028
Non-polymers5,87857
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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RNA chain , 2 types, 2 molecules A16SVTRF

#1: RNA chain RNA (1328-MER)


Mass: 486920.531 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: GenBank: 2440479486
#2: RNA chain RNA (26-MER)


Mass: 8411.946 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Haloferax volcanii (archaea) / Production host: Haloferax volcanii (archaea)

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30S ribosomal protein ... , 24 types, 24 molecules AS2PAS4EAS4PAS5PAS6EAS8ES11PS12PS15PS17PS24ES27ES3AEAS3PAS7PAS9PS10PS13PS14PS17ES19ES19PS27AS28E

#3: Protein 30S ribosomal protein S2


Mass: 22474.012 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps2, nbp1, Saci_0088
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P39478
#4: Protein 30S ribosomal protein S4e


Mass: 27339.988 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps4e, Saci_0585
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: O05634
#5: Protein 30S ribosomal protein S4


Mass: 19440.623 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps4, Saci_0081
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P39467
#6: Protein 30S ribosomal protein S5


Mass: 22782.686 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps5, Saci_0577
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: O05641
#7: Protein 30S ribosomal protein S6e


Mass: 11479.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps6e, Saci_0831
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAI1
#8: Protein 30S ribosomal protein S8e


Mass: 14113.179 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps8e, Saci_0758
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAP5
#9: Protein 30S ribosomal protein S11


Mass: 13652.649 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps11, Saci_0082
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P39469
#10: Protein 30S ribosomal protein S12


Mass: 15703.551 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps12, Saci_0688
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P11524
#11: Protein 30S ribosomal protein S15


Mass: 17438.426 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps15, Saci_0829
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAI3
#12: Protein 30S ribosomal protein S17


Mass: 12602.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps17, Saci_0588
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JB49
#13: Protein 30S ribosomal protein S24e


Mass: 10765.213 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps24e, Saci_0853
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAF9
#14: Protein 30S ribosomal protein S27e


Mass: 6576.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps27e, Saci_1276
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4J9B1
#15: Protein 30S ribosomal protein S3Ae / Ribosomal protein S1e


Mass: 21876.621 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps3ae, Saci_0620
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JB17
#16: Protein 30S ribosomal protein S3


Mass: 22396.506 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps3, Saci_0591
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JB46
#17: Protein 30S ribosomal protein S7


Mass: 21862.320 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps7, Saci_0686
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P17198
#18: Protein 30S ribosomal protein S9


Mass: 15638.136 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps9, Saci_0086
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P39468
#19: Protein 30S ribosomal protein S10


Mass: 11775.886 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps10, Saci_0684
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P17199
#20: Protein 30S ribosomal protein S13


Mass: 17140.783 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps13, Saci_0080
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: P39470
#21: Protein 30S ribosomal protein S14 type Z


Mass: 6190.371 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps14, Saci_0583
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: O05635
#22: Protein 30S ribosomal protein S17e


Mass: 7336.366 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps17e, Saci_0670
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAX5
#23: Protein 30S ribosomal protein S19e


Mass: 17559.660 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps19e
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4J8U1
#24: Protein 30S ribosomal protein S19


Mass: 13800.354 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps19, Saci_0593
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JB44
#25: Protein 30S ribosomal protein S27ae


Mass: 6261.392 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps27ae, Saci_0852
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAG0
#26: Protein 30S ribosomal protein S28e


Mass: 7094.255 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps28e, Saci_0698
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4JAV1

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Protein , 2 types, 2 molecules SL7AAS8P

#27: Protein 50S ribosomal protein L7Ae / Ribosomal protein L8e


Mass: 13351.481 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rpl7ae, Saci_1520
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: Q4J8P1
#28: Protein Small ribosomal subunit protein uS8 / 30S ribosomal protein S8


Mass: 14624.191 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus acidocaldarius DSM 639 (acidophilic)
Gene: rps8, Saci_0582
Production host: Sulfolobus acidocaldarius DSM 639 (acidophilic)
References: UniProt: O05636

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Non-polymers , 1 types, 57 molecules

#29: Chemical...
ChemComp-UNK / UNKNOWN


Type: L-peptide linking / Mass: 103.120 Da / Num. of mol.: 57 / Source method: obtained synthetically / Formula: C4H9NO2 / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: 2D ARRAY / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Sulfolobus acidocaldarius ribosome / Type: RIBOSOME / Entity ID: #2, #1, #3-#8, #28, #9-#28 / Source: NATURAL
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Buffer solutionpH: 7.5
SpecimenConc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 26.7 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 4.53 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 5281 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 460.55 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.003160729
ELECTRON MICROSCOPYf_angle_d0.738188678
ELECTRON MICROSCOPYf_chiral_restr0.042711005
ELECTRON MICROSCOPYf_plane_restr0.00426193
ELECTRON MICROSCOPYf_dihedral_angle_d19.491127229

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