+Open data
-Basic information
Entry | Database: PDB / ID: 8wik | ||||||||||||
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Title | Crystal structure of human FSP1 | ||||||||||||
Components | Ferroptosis suppressor protein 1 | ||||||||||||
Keywords | OXIDOREDUCTASE / FSP1 / Ferroptosis / FAD / NADH / inhibitor | ||||||||||||
Function / homology | Function and homology information electron-transferring-flavoprotein dehydrogenase activity / ubiquinone metabolic process / negative regulation of ferroptosis / Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor / vitamin K metabolic process / regulation of cellular response to oxidative stress / cellular detoxification / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / apoptotic mitochondrial changes / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release ...electron-transferring-flavoprotein dehydrogenase activity / ubiquinone metabolic process / negative regulation of ferroptosis / Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor / vitamin K metabolic process / regulation of cellular response to oxidative stress / cellular detoxification / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / apoptotic mitochondrial changes / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / lipid droplet / flavin adenine dinucleotide binding / mitochondrial outer membrane / positive regulation of apoptotic process / mitochondrion / DNA binding / extracellular space / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||||||||
Authors | Feng, S. / Huang, X. / Tang, D. / Qi, S. | ||||||||||||
Funding support | China, 3items
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Citation | Journal: MedComm (2020) / Year: 2024 Title: The crystal structure of human ferroptosis suppressive protein 1 in complex with flavin adenine dinucleotide and nicotinamide adenine nucleotide. Authors: Feng, S. / Huang, X. / Tang, D. / Liu, X. / Ouyang, L. / Yang, D. / Wang, K. / Liao, B. / Qi, S. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wik.cif.gz | 196.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wik.ent.gz | 129.7 KB | Display | PDB format |
PDBx/mmJSON format | 8wik.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/8wik ftp://data.pdbj.org/pub/pdb/validation_reports/wi/8wik | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40573.598 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: AIFM2, AMID, PRG3 / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: Q9BRQ8, Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor |
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#2: Chemical | ChemComp-6FA / |
#3: Chemical | ChemComp-NAD / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.05 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: HEPES , MgCl2, PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: 291 / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jan 15, 2023 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2→50 Å / Num. obs: 34829 / % possible obs: 100 % / Redundancy: 9.8 % / Biso Wilson estimate: 24.58 Å2 / CC1/2: 0.969 / CC star: 0.992 / Rmerge(I) obs: 0.118 / Rpim(I) all: 0.04 / Rrim(I) all: 0.125 / Χ2: 0.959 / Net I/σ(I): 4.6 / Num. measured all: 339711 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→35.26 Å / SU ML: 0.2611 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.0098 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.59 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→35.26 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 16.4241703844 Å / Origin y: -9.36275331131 Å / Origin z: -18.5992812359 Å
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Refinement TLS group | Selection details: (chain 'A' and resid 10 through 402) |