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- PDB-8wft: Crystal structure of beta-glucosidase from Thermoanaerobacterium ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8wft | ||||||
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Title | Crystal structure of beta-glucosidase from Thermoanaerobacterium saccharolyticum (Data 1) | ||||||
![]() | Beta-glucosidase | ||||||
![]() | HYDROLASE / glucosidase | ||||||
Function / homology | ![]() scopolin beta-glucosidase activity / beta-glucosidase / beta-glucosidase activity / cellulose catabolic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nam, K.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The Conformational Change of the L3 Loop Affects the Structural Changes in the Substrate Binding Pocket Entrance of beta-Glucosidase. Authors: Nam, K.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 404.7 KB | Display | ![]() |
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PDB format | ![]() | 328 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 479.4 KB | Display | ![]() |
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Full document | ![]() | 488.5 KB | Display | |
Data in XML | ![]() | 77.7 KB | Display | |
Data in CIF | ![]() | 112.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8wfuC ![]() 8wfvC ![]() 8wfwC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51807.148 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Tsac_2208 / Production host: ![]() ![]() #2: Chemical | ChemComp-TRS / #3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-NA / #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.09 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: Tris-HCl, PEG 4000, MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 19, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 123062 / % possible obs: 90.7 % / Redundancy: 3.7 % / CC1/2: 0.974 / Net I/σ(I): 10.78 |
Reflection shell | Resolution: 1.9→1.93 Å / Num. unique obs: 6212 / CC1/2: 0.563 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→49.89 Å
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Refine LS restraints |
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LS refinement shell |
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