+Open data
-Basic information
Entry | Database: PDB / ID: 8wfn | |||||||||
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Title | Cryo-EM structure of DSR2-TTP | |||||||||
Components |
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Keywords | ANTIVIRAL PROTEIN / cryo-EM structure of a protein | |||||||||
Biological species | Bacillus subtilis (bacteria) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.48 Å | |||||||||
Authors | Zhang, H. / Li, Z. / Li, X.Z. | |||||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Cryo-EM structure of DSR2-TTP Authors: Zhang, H. / Li, Z. / Li, X.Z. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wfn.cif.gz | 664.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wfn.ent.gz | 536.6 KB | Display | PDB format |
PDBx/mmJSON format | 8wfn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8wfn_validation.pdf.gz | 1.6 MB | Display | wwPDB validaton report |
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Full document | 8wfn_full_validation.pdf.gz | 1.6 MB | Display | |
Data in XML | 8wfn_validation.xml.gz | 99.5 KB | Display | |
Data in CIF | 8wfn_validation.cif.gz | 150.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wf/8wfn ftp://data.pdbj.org/pub/pdb/validation_reports/wf/8wfn | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 118525.695 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: WP_029317421.1 / Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: B4122_2870 / Production host: Escherichia coli (E. coli) #2: Protein | Mass: 29304.701 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: WP_010328117.1 / Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: B4122_1986 / Production host: Escherichia coli (E. coli) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: a protein / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Bacillus subtilis (bacteria) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING ONLY |
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3D reconstruction | Resolution: 4.48 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 200000 / Symmetry type: POINT |