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基本情報
登録情報 | データベース: PDB / ID: 8wa2 | ||||||
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タイトル | cryo-EM structure of native mastigonemes isolated from Chlamydomonas reinhardtii at 3.0 angstrom resolution | ||||||
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![]() | MEMBRANE PROTEIN / Cilia / arabinoglycan / PKD2 / PPII helix / mechanosensation. | ||||||
機能・相同性 | Tyrosine-protein kinase ephrin type A/B receptor-like / Tyrosine-protein kinase ephrin type A/B receptor-like / Putative ephrin-receptor like / Growth factor receptor cysteine-rich domain superfamily / alpha-L-arabinofuranose / alpha-D-galactopyranose / Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein![]() | ||||||
生物種 | ![]() ![]() synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3 Å | ||||||
![]() | Huang, J. / Tao, H. / Chen, J. / Pan, J. / Yan, C. / Yan, N. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure-guided discovery of protein and glycan components in native mastigonemes. 著者: Junhao Huang / Hui Tao / Jikun Chen / Yang Shen / Jianlin Lei / Junmin Pan / Chuangye Yan / Nieng Yan / ![]() 要旨: Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we ...Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we report the cryo-EM structure of native mastigonemes isolated from Chlamydomonas at 3.0 Å resolution. The long stem assembles as a super spiral, with each helical turn comprising four pairs of anti-parallel mastigoneme-like protein 1 (Mst1). A large array of arabinoglycans, which represents a common class of glycosylation in plants and algae, is resolved surrounding the type II poly-hydroxyproline (Hyp) helix in Mst1. The EM map unveils a mastigoneme axial protein (Mstax) that is rich in heavily glycosylated Hyp and contains a PKD2-like transmembrane domain (TMD). Mstax, with nearly 8,000 residues spanning from the intracellular region to the distal end of the mastigoneme, provides the framework for Mst1 assembly. Our study provides insights into the complexity of protein and glycan interactions in native bio-architectures. | ||||||
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PDBx/mmCIF形式 | ![]() | 2.1 MB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 9.8 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 10.3 MB | 表示 | |
XML形式データ | ![]() | 290.9 KB | 表示 | |
CIF形式データ | ![]() | 448.8 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 37389MC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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要素
-タンパク質 , 2種, 9分子 ABCDEFGHI
#1: タンパク質 | 分子量: 206316.203 Da / 分子数: 6 / 由来タイプ: 天然 由来: (天然) ![]() ![]() 参照: UniProt: A8J9H7 #2: タンパク質 | 分子量: 6239.464 Da / 分子数: 3 / 由来タイプ: 合成 詳細: Our Mstax sequence in the structure (chain G, H and I) represents the average sequence resulting from the statistical analysis of a segment of 34 repeated sequences in Mstax. 由来: (合成) synthetic construct (人工物) |
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+糖 , 25種, 444分子 ![](data/chem/img/AHR.gif)
![](data/chem/img/GLA.gif)
![](data/chem/img/NAG.gif)
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![](data/chem/img/NAG.gif)