[English] 日本語
![](img/lk-miru.gif)
- PDB-8w6v: Structural basis of chorismate isomerization by Arabidopsis isoch... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8w6v | ||||||
---|---|---|---|---|---|---|---|
Title | Structural basis of chorismate isomerization by Arabidopsis isochorismate synthase ICS1 | ||||||
![]() | Isochorismate synthase 1, chloroplastic![]() | ||||||
![]() | ![]() | ||||||
Function / homology | ![]() leaf abscission / negative regulation of defense response / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Su, Z.H. / Ming, Z.H. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structural basis of chorismate isomerization by Arabidopsis ISOCHORISMATE SYNTHASE1. Authors: Su, Z. / Niu, C. / Zhou, S. / Xu, G. / Zhu, P. / Fu, Q. / Zhang, Y. / Ming, Z. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 233.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 180.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 8w71C C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | ![]() Mass: 59225.750 Da / Num. of mol.: 2 / Mutation: K316R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Production host: ![]() ![]() ![]() References: UniProt: Q9S7H8, ![]() #2: Chemical | ![]() #3: Chemical | ChemComp-FMT / ![]() #4: Chemical | #5: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.82 Å3/Da / Density % sol: 67.79 % |
---|---|
Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 2.8M sodium formate, 0.08M sodium acetate trihydrate at pH 4.6, 0.08M sodium malonate at pH 6.0, 0.02M MES monohydrate at pH 6.0, 0.1% w/v Polyethylene glycol 10000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 2, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→19.82 Å / Num. obs: 144297 / % possible obs: 96.6 % / Redundancy: 3.5 % / CC1/2: 0.997 / Rmerge(I) obs: 0.065 / Net I/σ(I): 12.2 |
Reflection shell | Resolution: 1.8→1.83 Å / Num. unique obs: 7079 / CC1/2: 0.888 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]()
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→19.82 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|