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- PDB-8w3h: Crystal structure of prefusion-stabilized RSV F protein UFCR1-iSS -
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Open data
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Basic information
Entry | Database: PDB / ID: 8w3h | ||||||
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Title | Crystal structure of prefusion-stabilized RSV F protein UFCR1-iSS | ||||||
![]() | Prefusion-stabilized RSV F protein UFCR1-iSS | ||||||
![]() | VIRAL PROTEIN / Respiratory syncytial virus / glycoprotein / prefusion vaccine / stabilized | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, Y.Z. / Stanfield, R.L. / Wilson, I.A. / Zhu, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Rational design of uncleaved prefusion-closed trimer vaccines for human respiratory syncytial virus and metapneumovirus. Authors: Lee, Y.Z. / Han, J. / Zhang, Y.N. / Ward, G. / Braz Gomes, K. / Auclair, S. / Stanfield, R.L. / He, L. / Wilson, I.A. / Zhu, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117.3 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 464.4 KB | Display | ![]() |
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Full document | ![]() | 474.9 KB | Display | |
Data in XML | ![]() | 23.5 KB | Display | |
Data in CIF | ![]() | 32 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8w3eC ![]() 8w3fC ![]() 8w3gC ![]() 8w3iC ![]() 8w3jC ![]() 8w3kC ![]() 8w3lC ![]() 8w3nC ![]() 8w3oC ![]() 8w3pC ![]() 8w3qC ![]() 8w3rC C: citing same article ( |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 55684.719 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||
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#2: Sugar | ChemComp-NAG / | ||||||
#3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | N | Has protein modification | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.61 Å3/Da / Density % sol: 65.94 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M Potassium Sodium tartrate, 16% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 22, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→37.55 Å / Num. obs: 38718 / % possible obs: 99.94 % / Redundancy: 32.8 % / Biso Wilson estimate: 27.36 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.1413 / Net I/σ(I): 22.67 |
Reflection shell | Resolution: 2.254→2.335 Å / Num. unique obs: 3809 / CC1/2: 0.999 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.2 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.25→37.55 Å
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Refine LS restraints |
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LS refinement shell |
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