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- PDB-8w0y: Crystal structure of broadly neutralizing antibody hcab17 in comp... -

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Basic information

Entry
Database: PDB / ID: 8w0y
TitleCrystal structure of broadly neutralizing antibody hcab17 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Components
  • Envelope glycoprotein E2
  • hcab17 Fab Heavy Chain
  • hcab17 Fab Light Chain
KeywordsIMMUNE SYSTEM/VIRAL PROTEIN / HCV glycoprotein / broadly neutralizing antibodies / IMMUNE SYSTEM / IMMUNE SYSTEM-VIRAL PROTEIN complex
Function / homology
Function and homology information


host cell lipid droplet / host cell mitochondrion / viral nucleocapsid / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host innate immune response / symbiont entry into host cell / ribonucleoprotein complex / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell ...host cell lipid droplet / host cell mitochondrion / viral nucleocapsid / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host innate immune response / symbiont entry into host cell / ribonucleoprotein complex / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell nucleus / virion membrane / structural molecule activity
Similarity search - Function
Hepatitis C virus, Core protein, C-terminal / Hepatitis C virus core protein / Hepatitis C virus, Non-structural protein E2/NS1 / Hepatitis C virus non-structural protein E2/NS1 / Hepatitis C virus, Envelope glycoprotein E1 / Hepatitis C virus envelope glycoprotein E1
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Hepacivirus hominis
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.31 Å
AuthorsFlyak, A.I. / Wilcox, X.E.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)U19 AI159822 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R00 AI153465 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)K99 AI153465 United States
CitationJournal: Immunity / Year: 2024
Title: Convergent evolution and targeting of diverse E2 epitopes by human broadly neutralizing antibodies are associated with HCV clearance.
Authors: Ogega, C.O. / Skinner, N.E. / Schoenle, M.V. / Wilcox, X.E. / Frumento, N. / Wright, D.A. / Paul, H.T. / Sinnis-Bourozikas, A. / Clark, K.E. / Figueroa, A. / Bjorkman, P.J. / Ray, S.C. / ...Authors: Ogega, C.O. / Skinner, N.E. / Schoenle, M.V. / Wilcox, X.E. / Frumento, N. / Wright, D.A. / Paul, H.T. / Sinnis-Bourozikas, A. / Clark, K.E. / Figueroa, A. / Bjorkman, P.J. / Ray, S.C. / Flyak, A.I. / Bailey, J.R.
History
DepositionFeb 14, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 27, 2024Provider: repository / Type: Initial release
Revision 1.1Apr 3, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Apr 17, 2024Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: hcab17 Fab Heavy Chain
B: hcab17 Fab Light Chain
C: Envelope glycoprotein E2
D: Envelope glycoprotein E2
H: hcab17 Fab Heavy Chain
L: hcab17 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)160,38717
Polymers155,1116
Non-polymers5,27611
Water724
1
A: hcab17 Fab Heavy Chain
B: hcab17 Fab Light Chain
D: Envelope glycoprotein E2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)79,8408
Polymers77,5563
Non-polymers2,2845
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8100 Å2
ΔGint3 kcal/mol
Surface area32580 Å2
MethodPISA
2
C: Envelope glycoprotein E2
H: hcab17 Fab Heavy Chain
L: hcab17 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)80,5479
Polymers77,5563
Non-polymers2,9926
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9230 Å2
ΔGint12 kcal/mol
Surface area33220 Å2
MethodPISA
Unit cell
Length a, b, c (Å)179.410, 179.410, 168.762
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number150
Space group name H-MP321

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Components

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Antibody , 2 types, 4 molecules AHBL

#1: Antibody hcab17 Fab Heavy Chain


Mass: 25217.068 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pTT5 / Cell line (production host): HEK293expi / Production host: Homo sapiens (human)
#2: Antibody hcab17 Fab Light Chain


Mass: 23392.049 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pTT5 / Cell line (production host): HEK293expi / Production host: Homo sapiens (human)

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Protein / Non-polymers , 2 types, 6 molecules CD

#3: Protein Envelope glycoprotein E2


Mass: 28946.479 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Hepacivirus hominis / Strain: 1b09 / Plasmid: pCMV / Cell line (production host): HEK293expi / Production host: Homo sapiens (human) / References: UniProt: A0A2P0NE15
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: H2O

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Sugars , 4 types, 11 molecules

#4: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 7
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#5: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#6: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#7: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 5.05 Å3/Da / Density % sol: 75.67 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 1.0 M Sodium citrate tribasic dihydrate and 0.1 M Sodium cacodylate

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Data collection

DiffractionMean temperature: 298 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å
DetectorType: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Sep 21, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.31→89.71 Å / Num. obs: 47162 / % possible obs: 100 % / Redundancy: 9.8 % / CC1/2: 0.97 / Rmerge(I) obs: 0.483 / Rpim(I) all: 0.163 / Rrim(I) all: 0.51 / Χ2: 0.95 / Net I/σ(I): 3.7 / Num. measured all: 459905
Reflection shellResolution: 3.31→3.43 Å / % possible obs: 100 % / Redundancy: 8.9 % / Rmerge(I) obs: 3.032 / Num. measured all: 40627 / Num. unique obs: 4577 / CC1/2: 0.235 / Rpim(I) all: 1.063 / Rrim(I) all: 3.216 / Χ2: 0.9 / Net I/σ(I) obs: 1.1

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
Aimlessdata scaling
iMOSFLMdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.31→77.69 Å / SU ML: 0.46 / Cross valid method: FREE R-VALUE / σ(F): 0.8 / Phase error: 26.65 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.253 2248 4.77 %
Rwork0.2094 --
obs0.2115 47086 99.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.31→77.69 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10443 0 349 4 10796
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00811091
X-RAY DIFFRACTIONf_angle_d1.09815130
X-RAY DIFFRACTIONf_dihedral_angle_d9.0071692
X-RAY DIFFRACTIONf_chiral_restr0.0611728
X-RAY DIFFRACTIONf_plane_restr0.011917
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.31-3.380.37561380.33452773X-RAY DIFFRACTION100
3.38-3.460.31461280.31272759X-RAY DIFFRACTION99
3.46-3.550.31761480.29642735X-RAY DIFFRACTION100
3.55-3.640.30621530.272789X-RAY DIFFRACTION100
3.64-3.750.27971310.26952772X-RAY DIFFRACTION100
3.75-3.870.3191510.25272746X-RAY DIFFRACTION100
3.87-4.010.291450.23432798X-RAY DIFFRACTION100
4.01-4.170.27251440.21052792X-RAY DIFFRACTION100
4.17-4.360.24841320.19462783X-RAY DIFFRACTION100
4.36-4.590.22871570.17832793X-RAY DIFFRACTION100
4.59-4.880.22781560.17112765X-RAY DIFFRACTION100
4.88-5.250.18731280.16612828X-RAY DIFFRACTION100
5.25-5.780.21951040.17282850X-RAY DIFFRACTION100
5.78-6.620.26891470.20262836X-RAY DIFFRACTION100
6.62-8.330.23371340.19422855X-RAY DIFFRACTION100
8.34-77.690.22291520.19042964X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.84187.29856.80227.27935.92245.2080.1885-0.93140.75230.4256-1.4346-0.53040.1346-0.70711.32720.89370.03620.10461.0325-0.14781.093959.140342.806734.2218
21.9791-3.14012.6674.456-4.37547.0630.3751-0.2266-0.2534-0.28-0.30991.61961.65410.7542-0.4441.1077-0.2359-0.17570.9809-0.341.306453.148816.833839.382
37.9034-1.4151-2.17910.66272.35877.10410.6007-0.2955-0.87560.5838-0.0770.05790.6411-0.2953-0.40041.6731-0.12630.01770.7450.00141.317679.13584.342144.0842
43.36590.61411.3971.1639-0.20964.94530.1358-0.0051-0.4903-0.0407-0.01630.20630.2938-0.1278-0.16340.8849-0.1563-0.01710.5788-0.10220.861674.093717.913239.3648
52.77451.1941-0.38320.3025-0.1891.1187-0.0778-0.10730.81370.24120.2064-0.59750.70330.9839-0.09881.06390.2883-0.18930.9545-0.07791.294776.063522.061863.3831
61.73662.48832.80684.80085.06785.59670.32350.4088-0.87140.7826-0.60220.04631.2537-1.2920.16911.2284-0.23080.26111.2666-0.39261.314749.6619.720753.7439
78.27273.90813.41835.92614.74654.20670.44750.0062-0.13140.408-0.68580.69420.3478-1.85780.26450.96490.0183-0.12541.4587-0.06231.040641.621347.131850.0553
81.0776-0.3931-1.04458.067-6.03617.05030.04480.2895-0.13470.53120.24960.5989-0.1996-0.9214-0.27110.6757-0.1215-0.01630.9423-0.21320.79750.232136.447258.054
92.6222-1.25712.01132.8325-1.22765.98910.0133-0.0017-0.2639-0.3293-0.03910.28340.2155-0.13520.02380.4413-0.09230.00460.6392-0.09610.667555.057140.946953.9577
103.9205-0.6443-0.76545.3869-4.78019.2833-0.290.27510.3255-0.49230.03430.45950.4614-0.30080.30051.03880.1352-0.09070.8322-0.14640.648377.634832.3258.294
117.54661.4978-3.5366.3775-1.92189.4025-0.0657-0.60020.27330.33410.2151-0.2259-0.56370.9984-0.16330.69130.0346-0.03690.5929-0.03790.481184.897737.162418.1615
124.0036-0.89970.52533.2249-0.95499.27460.2877-0.2778-0.0760.1626-0.14690.23420.0517-0.8309-0.07380.5758-0.05510.00760.4749-0.04820.654383.004535.240118.8237
130.7408-2.34240.4487.84090.57074.34130.26360.6462-0.2148-1.2942-0.05370.15880.0514-0.2705-0.24360.6708-0.1303-0.0380.7918-0.04210.642286.39520.9605-9.3254
144.644-3.64253.45493.005-3.697.45770.77030.26630.039-2.1201-0.5391-0.438-0.8350.2085-0.29320.9707-0.11480.070.7925-0.10580.788892.328210.7662-13.0927
151.8733-1.89041.53458.8697-0.06663.2041-0.070.3987-0.1661-0.14440.0830.2014-0.4711-0.3595-0.02891.01-0.04650.07470.9347-0.0720.656285.90311.929-9.8844
161.9997-1.04781.99951.99973.33171.999910.8847-15.722326.55654.4981-6.870621.1522-11.657-0.6946-4.08792.42560.39870.47911.4488-0.56211.254394.23652.625-27.6818
174.59590.1591-0.29863.04850.84926.2979-0.2254-0.11520.049-0.05890.0410.0844-0.05-0.18190.16510.40590.0435-0.02280.40180.02390.578771.752746.657879.7094
182.49110.24953.50294.1242-2.28716.5686-1.9979-1.79831.34871.89461.41280.37450.4625-0.24230.57930.74170.3401-0.06631.4476-0.19540.937648.421860.9363109.9418
198.8513-2.28911.89981.3359-1.23114.8387-0.0902-0.5527-0.67580.21980.05660.35650.32370.10420.04380.81420.16380.07380.90830.01310.783849.174653.5507104.2446
201.4883-2.2726-2.72144.21125.356.72240.3692-0.1876-0.4495-1.0223-0.21860.40.3270.4062-0.09960.93270.1811-0.07810.784-0.04410.9406106.065423.621917.1447
215.67872.1592-0.79159.4233-2.60380.69640.1251-0.09110.11640.50150.058-0.43690.32660.2703-0.1050.89880.1272-0.10490.7579-0.06290.5356101.327630.07723.8374
227.2512-0.2272-2.15161.9224-2.67094.64610.0549-0.3545-0.1270.1552-0.27880.12290.77660.24410.19540.80690.0522-0.12480.4305-0.13760.639197.768321.017523.6046
236.11475.53414.82445.52993.08018.9891-0.34760.01781.50261.3641-0.14770.0814-0.29890.11650.35950.5926-0.1374-0.02070.6962-0.1551.061497.535136.819520.0809
242.4251-1.8699-0.16233.60011.78086.45870.61760.2669-0.1961-0.36010.004-0.07410.5629-0.2299-0.59621.0317-0.20570.1250.8257-0.12970.770199.358512.323-9.5272
253.2409-1.48780.76386.7813-4.6134.50620.10870.1504-0.0516-0.3465-0.0118-0.7353-0.2290.7175-0.03310.9992-0.11680.08840.9838-0.13140.9367104.204315.6577-9.8841
262.61431.7836-1.94049.5679-6.20436.8214-0.92480.7746-0.7014-0.31350.1943-1.58150.0528-0.14610.66220.9909-0.07210.15360.7012-0.22771.0495102.042816.7202-6.275
275.9057-3.35213.90376.9545-4.94663.94230.04481.04460.6122-0.6816-0.2163-0.6762-1.42641.31350.14851.2207-0.01770.18850.9491-0.15560.9389102.544718.9982-20.9506
282.2059-1.96351.90652.6214-2.40772.18270.72242.28590.202-0.23430.03280.11370.00352.9325-0.80191.256-0.09130.38411.61770.08471.2386108.521210.3635-17.553
296.919-0.9842-4.57246.54191.13014.34020.2022-0.00610.6232-0.3039-0.20330.32-0.6758-0.23840.0720.64180.1061-0.10550.5179-0.04020.664967.57769.909473.332
301.5515-0.5106-1.98044.02122.85375.71220.24830.55630.1648-0.1254-0.34620.2269-0.2908-0.99340.10650.41250.079-0.06250.7180.06830.661361.445364.110673.5772
316.0762-2.58684.58885.0603-3.15894.883-0.7005-0.8971.05081.33540.5159-0.4613-0.93620.10410.24380.840.2551-0.08891.0654-0.39181.090255.224867.354108.6949
321.8981-0.5626-1.74493.7189-1.4364.9951-0.3348-1.1163-0.25930.19390.3140.36970.4693-0.47020.2110.62680.11950.14821.0538-0.24451.086457.808166.0577103.8812
332.8404-3.68112.89588.8872-7.10295.434-0.8627-0.61720.13332.43760.5782-0.544-2.57930.2350.14551.39080.2292-0.19921.0336-0.33511.152354.539974.4904112.9216
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'D' and (resid 404 through 423 )
2X-RAY DIFFRACTION2chain 'D' and (resid 424 through 450 )
3X-RAY DIFFRACTION3chain 'D' and (resid 451 through 484 )
4X-RAY DIFFRACTION4chain 'D' and (resid 485 through 645 )
5X-RAY DIFFRACTION5chain 'C' and (resid 399 through 424 )
6X-RAY DIFFRACTION6chain 'C' and (resid 425 through 451 )
7X-RAY DIFFRACTION7chain 'C' and (resid 452 through 484 )
8X-RAY DIFFRACTION8chain 'C' and (resid 485 through 509 )
9X-RAY DIFFRACTION9chain 'C' and (resid 510 through 645 )
10X-RAY DIFFRACTION10chain 'A' and (resid 1 through 22 )
11X-RAY DIFFRACTION11chain 'A' and (resid 23 through 66 )
12X-RAY DIFFRACTION12chain 'A' and (resid 67 through 103 )
13X-RAY DIFFRACTION13chain 'A' and (resid 104 through 130 )
14X-RAY DIFFRACTION14chain 'A' and (resid 131 through 145 )
15X-RAY DIFFRACTION15chain 'A' and (resid 146 through 214 )
16X-RAY DIFFRACTION16chain 'A' and (resid 215 through 226 )
17X-RAY DIFFRACTION17chain 'H' and (resid 1 through 120 )
18X-RAY DIFFRACTION18chain 'H' and (resid 121 through 145 )
19X-RAY DIFFRACTION19chain 'H' and (resid 146 through 225 )
20X-RAY DIFFRACTION20chain 'B' and (resid 170 through 187 )
21X-RAY DIFFRACTION21chain 'B' and (resid 188 through 207 )
22X-RAY DIFFRACTION22chain 'B' and (resid 208 through 259 )
23X-RAY DIFFRACTION23chain 'B' and (resid 260 through 271 )
24X-RAY DIFFRACTION24chain 'B' and (resid 272 through 297 )
25X-RAY DIFFRACTION25chain 'B' and (resid 298 through 319 )
26X-RAY DIFFRACTION26chain 'B' and (resid 320 through 343 )
27X-RAY DIFFRACTION27chain 'B' and (resid 344 through 367 )
28X-RAY DIFFRACTION28chain 'B' and (resid 368 through 382 )
29X-RAY DIFFRACTION29chain 'L' and (resid 170 through 201 )
30X-RAY DIFFRACTION30chain 'L' and (resid 202 through 282 )
31X-RAY DIFFRACTION31chain 'L' and (resid 283 through 321 )
32X-RAY DIFFRACTION32chain 'L' and (resid 322 through 355 )
33X-RAY DIFFRACTION33chain 'L' and (resid 356 through 382 )

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