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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 8vj0 | ||||||||||||
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タイトル | Neutron Structure of Oxidized Trp161Phe MnSOD | ||||||||||||
![]() | Superoxide dismutase [Mn], mitochondrial | ||||||||||||
![]() | OXIDOREDUCTASE / MnSOD / SOD2 / PCET | ||||||||||||
機能・相同性 | ![]() acetylcholine-mediated vasodilation involved in regulation of systemic arterial blood pressure / erythrophore differentiation / positive regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching / negative regulation of membrane hyperpolarization / detection of oxygen / positive regulation of hydrogen peroxide biosynthetic process / response to magnetism / response to silicon dioxide / response to L-ascorbic acid / response to isolation stress ...acetylcholine-mediated vasodilation involved in regulation of systemic arterial blood pressure / erythrophore differentiation / positive regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching / negative regulation of membrane hyperpolarization / detection of oxygen / positive regulation of hydrogen peroxide biosynthetic process / response to magnetism / response to silicon dioxide / response to L-ascorbic acid / response to isolation stress / intracellular oxygen homeostasis / response to selenium ion / response to superoxide / cellular response to ethanol / superoxide anion generation / hydrogen peroxide biosynthetic process / response to manganese ion / intrinsic apoptotic signaling pathway in response to oxidative stress / positive regulation of vascular associated smooth muscle cell apoptotic process / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / superoxide metabolic process / response to zinc ion / negative regulation of fat cell differentiation / superoxide dismutase / Detoxification of Reactive Oxygen Species / mitochondrial nucleoid / superoxide dismutase activity / negative regulation of vascular associated smooth muscle cell proliferation / hemopoiesis / response to immobilization stress / response to axon injury / response to hyperoxia / neuron development / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / response to cadmium ion / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / response to electrical stimulus / negative regulation of fibroblast proliferation / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / respiratory electron transport chain / removal of superoxide radicals / glutathione metabolic process / release of cytochrome c from mitochondria / post-embryonic development / liver development / regulation of mitochondrial membrane potential / response to activity / locomotory behavior / response to gamma radiation / response to hydrogen peroxide / Transcriptional activation of mitochondrial biogenesis / oxygen binding / multicellular organismal-level iron ion homeostasis / regulation of blood pressure / intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of nitric oxide biosynthetic process / heart development / manganese ion binding / cellular response to oxidative stress / protein homotetramerization / negative regulation of neuron apoptotic process / response to lipopolysaccharide / response to hypoxia / positive regulation of cell migration / mitochondrial matrix / response to xenobiotic stimulus / negative regulation of cell population proliferation / regulation of transcription by RNA polymerase II / enzyme binding / mitochondrion / DNA binding / extracellular exosome / identical protein binding 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() | ||||||||||||
手法 | 中性子回折 / ![]() | ||||||||||||
![]() | Azadmanesh, J. / Slobodnik, K. / Struble, L.R. / Lutz, W.E. / Weiss, K.L. / Myles, D.A.A. / Kroll, T. / Borgstahl, G.E.O. | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Revealing the atomic and electronic mechanism of human manganese superoxide dismutase product inhibition. 著者: Azadmanesh, J. / Slobodnik, K. / Struble, L.R. / Lutz, W.E. / Coates, L. / Weiss, K.L. / Myles, D.A.A. / Kroll, T. / Borgstahl, G.E.O. | ||||||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 190.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 133.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 343 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 343.6 KB | 表示 | |
XML形式データ | ![]() | 8.7 KB | 表示 | |
CIF形式データ | ![]() | 13.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
#1: タンパク質 | 分子量: 22325.271 Da / 分子数: 2 / 変異: W161F / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: 化合物 | #3: 水 | ChemComp-HOH / | 研究の焦点であるリガンドがあるか | N | |
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-実験情報
-実験
実験 | 手法: 中性子回折 / 使用した結晶の数: 1 |
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試料調製
結晶化 | 温度: 296 K / 手法: 蒸気拡散法, シッティングドロップ法 / 詳細: 1.93 M Potassium Phosphate |
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-データ収集
回折 | 平均測定温度: 296 K / Serial crystal experiment: N | |||||||||
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放射光源 | 由来: SPALLATION SOURCE / サイト: ORNL Spallation Neutron Source ![]() | |||||||||
検出器 | タイプ: ORNL ANGER CAMERA / 検出器: DIFFRACTOMETER / 日付: 2023年9月1日 | |||||||||
放射 | プロトコル: LAUE / 単色(M)・ラウエ(L): L / 散乱光タイプ: neutron | |||||||||
放射波長 |
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反射 | 解像度: 2.3→14.89 Å / Num. obs: 20489 / % possible obs: 96.6 % / 冗長度: 6 % / Biso Wilson estimate: -4.19 Å2 / CC1/2: 0.978 / Rpim(I) all: 0.083 / Net I/σ(I): 5.9 | |||||||||
反射 シェル | 解像度: 2.3→2.42 Å / Mean I/σ(I) obs: 3.8 / Num. unique obs: 1972 / CC1/2: 0.368 / Rpim(I) all: 0.125 |
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解析
精密化 | 構造決定の手法: ![]() 立体化学のターゲット値: GeoStd + Monomer Library + CDL v1.2
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 17.42 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
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LS精密化 シェル |
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