[English] 日本語
Yorodumi
- PDB-8v4z: Crystal structure of a HLA-B*35:01-NP7 with D1 TCR -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 8v4z
TitleCrystal structure of a HLA-B*35:01-NP7 with D1 TCR
Components
  • Beta-2-microglobulin
  • D1 TCR alpha chain
  • D1 TCR beta chain
  • LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
  • MHC class I antigen
KeywordsIMMUNE SYSTEM / HLA B*3501 / NP418 epitope / T cell immunity / glycoprotein / host-virus interaction / immune response
Function / homology
Function and homology information


antigen processing and presentation of peptide antigen via MHC class I / negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / lumenal side of endoplasmic reticulum membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC ...antigen processing and presentation of peptide antigen via MHC class I / negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / lumenal side of endoplasmic reticulum membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / MHC class II protein complex / negative regulation of forebrain neuron differentiation / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / regulation of erythrocyte differentiation / HFE-transferrin receptor complex / response to molecule of bacterial origin / MHC class I peptide loading complex / T cell mediated cytotoxicity / positive regulation of T cell cytokine production / antigen processing and presentation of endogenous peptide antigen via MHC class I / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / MHC class I protein complex / positive regulation of T cell activation / peptide antigen binding / positive regulation of receptor-mediated endocytosis / negative regulation of neurogenesis / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / multicellular organismal-level iron ion homeostasis / specific granule lumen / phagocytic vesicle membrane / recycling endosome membrane / Interferon gamma signaling / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / negative regulation of epithelial cell proliferation / MHC class II protein complex binding / Modulation by Mtb of host immune system / late endosome membrane / sensory perception of smell / positive regulation of cellular senescence / tertiary granule lumen / DAP12 signaling / T cell differentiation in thymus / negative regulation of neuron projection development / ER-Phagosome pathway / protein refolding / early endosome membrane / protein homotetramerization / amyloid fibril formation / intracellular iron ion homeostasis / learning or memory / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / lysosomal membrane / external side of plasma membrane / focal adhesion / Neutrophil degranulation / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / endoplasmic reticulum / Golgi apparatus / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / plasma membrane / cytosol
Similarity search - Function
MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. ...MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
PHOSPHATE ION / MHC class I antigen / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
Influenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.40000208775 Å
AuthorsLittler, D.R. / Rossjohn, J.
Funding support Australia, 1items
OrganizationGrant numberCountry
Australian Research Council (ARC) Australia
CitationJournal: Sci Immunol / Year: 2025
Title: Molecular determinants of cross-strain influenza A virus recognition by alpha beta T cell receptors.
Authors: Quinones-Parra, S.M. / Gras, S. / Nguyen, T.H.O. / Farenc, C. / Szeto, C. / Rowntree, L.C. / Chaurasia, P. / Sant, S. / Boon, A.C.M. / Jayasinghe, D. / Rimmelzwaan, G.F. / Petersen, J. / ...Authors: Quinones-Parra, S.M. / Gras, S. / Nguyen, T.H.O. / Farenc, C. / Szeto, C. / Rowntree, L.C. / Chaurasia, P. / Sant, S. / Boon, A.C.M. / Jayasinghe, D. / Rimmelzwaan, G.F. / Petersen, J. / Doherty, P.C. / Uldrich, A.P. / Littler, D.R. / Rossjohn, J. / Kedzierska, K.
History
DepositionNov 29, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 18, 2024Provider: repository / Type: Initial release
Revision 1.1Jan 1, 2025Group: Derived calculations / Category: pdbx_struct_assembly / pdbx_struct_assembly_gen
Revision 1.2Feb 26, 2025Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: MHC class I antigen
B: Beta-2-microglobulin
C: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
D: D1 TCR alpha chain
E: D1 TCR beta chain
F: MHC class I antigen
G: Beta-2-microglobulin
H: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
I: D1 TCR alpha chain
J: D1 TCR beta chain
K: MHC class I antigen
L: Beta-2-microglobulin
M: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
N: D1 TCR alpha chain
O: D1 TCR beta chain
P: MHC class I antigen
Q: Beta-2-microglobulin
R: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
S: D1 TCR alpha chain
T: D1 TCR beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)375,96424
Polymers375,58420
Non-polymers3804
Water13,872770
1
A: MHC class I antigen
B: Beta-2-microglobulin
C: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
D: D1 TCR alpha chain
E: D1 TCR beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,9916
Polymers93,8965
Non-polymers951
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
F: MHC class I antigen
G: Beta-2-microglobulin
H: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
I: D1 TCR alpha chain
J: D1 TCR beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,9916
Polymers93,8965
Non-polymers951
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
K: MHC class I antigen
L: Beta-2-microglobulin
M: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
N: D1 TCR alpha chain
O: D1 TCR beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,9916
Polymers93,8965
Non-polymers951
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
P: MHC class I antigen
Q: Beta-2-microglobulin
R: LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET
S: D1 TCR alpha chain
T: D1 TCR beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,9916
Polymers93,8965
Non-polymers951
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)78.988, 190.209, 250.430
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
12
22
32
42
13
23
33
43
14
24
34
44
15
25
35
45

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLNGLNGLNGLN(chain I and (resseq 2:6 or resseq 8:22 or resseq...II2 - 61 - 5
121PROPROASNASN(chain I and (resseq 2:6 or resseq 8:22 or resseq...II8 - 226 - 20
131THRTHRGLYGLY(chain I and (resseq 2:6 or resseq 8:22 or resseq...II24 - 2922 - 27
141PHEPHELEULEU(chain I and (resseq 2:6 or resseq 8:22 or resseq...II36 - 5828 - 50
151ASPASPTHRTHR(chain I and (resseq 2:6 or resseq 8:22 or resseq...II63 - 6851 - 56
161GLYGLYPHEPHE(chain I and (resseq 2:6 or resseq 8:22 or resseq...II74 - 10357 - 86
171ALAALAPHEPHE(chain I and (resseq 2:6 or resseq 8:22 or resseq...II105 - 21288 - 195
181PROPROPROPRO(chain I and (resseq 2:6 or resseq 8:22 or resseq...II214197
291GLNGLNGLNGLN(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD2 - 61 - 5
2101PROPROASNASN(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD8 - 226 - 20
2111THRTHRGLYGLY(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD24 - 2922 - 27
2121PHEPHELEULEU(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD36 - 5828 - 50
2131ASPASPTHRTHR(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD63 - 6851 - 56
2141GLYGLYPHEPHE(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD74 - 10357 - 86
2151ALAALAPHEPHE(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD105 - 21288 - 195
2161PROPROPROPRO(chain D and (resseq 2:6 or resseq 8:22 or resseq...DD214197
3171GLNGLNGLNGLN(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN2 - 61 - 5
3181PROPROASNASN(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN8 - 226 - 20
3191THRTHRGLYGLY(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN24 - 2922 - 27
3201PHEPHELEULEU(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN36 - 5828 - 50
3211ASPASPTHRTHR(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN63 - 6851 - 56
3221GLYGLYPHEPHE(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN74 - 10357 - 86
3231ALAALAPHEPHE(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN105 - 21288 - 195
3241PROPROPROPRO(chain N and (resseq 2:6 or resseq 8:22 or resseq...NN214197
4251GLNGLNGLNGLN(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS2 - 61 - 5
4261PROPROASNASN(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS8 - 226 - 20
4271THRTHRGLYGLY(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS24 - 2922 - 27
4281PHEPHELEULEU(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS36 - 5828 - 50
4291ASPASPTHRTHR(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS63 - 6851 - 56
4301GLYGLYPHEPHE(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS74 - 10357 - 86
4311ALAALAPHEPHE(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS105 - 21288 - 195
4321PROPROPROPRO(chain S and (resseq 2:6 or resseq 8:22 or resseq...SS214197
1332SERSERTHRTHR(chain A and (resseq 2:6 or (resid 7 and (name...AA2 - 102 - 10
1342SERSERTRPTRP(chain A and (resseq 2:6 or (resid 7 and (name...AA13 - 6013 - 60
1352ARGARGGLNGLN(chain A and (resseq 2:6 or (resid 7 and (name...AA62 - 6562 - 65
1362PHEPHETYRTYR(chain A and (resseq 2:6 or (resid 7 and (name...AA67 - 7467 - 74
1372GLUGLUARGARG(chain A and (resseq 2:6 or (resid 7 and (name...AA76 - 9776 - 97
1382TYRTYRGLNGLN(chain A and (resseq 2:6 or (resid 7 and (name...AA99 - 14499 - 144
1392LYSLYSTHRTHR(chain A and (resseq 2:6 or (resid 7 and (name...AA146 - 190146 - 190
1402PROPROGLNGLN(chain A and (resseq 2:6 or (resid 7 and (name...AA193 - 218193 - 218
1412ASPASPGLNGLN(chain A and (resseq 2:6 or (resid 7 and (name...AA220 - 255220 - 255
1422TYRTYRLEULEU(chain A and (resseq 2:6 or (resid 7 and (name...AA257 - 272257 - 272
2432SERSERTHRTHR(chain F and (resseq 2:6 or (resid 7 and (name...FF2 - 102 - 10
2442SERSERTRPTRP(chain F and (resseq 2:6 or (resid 7 and (name...FF13 - 6013 - 60
2452ARGARGGLNGLN(chain F and (resseq 2:6 or (resid 7 and (name...FF62 - 6562 - 65
2462PHEPHETYRTYR(chain F and (resseq 2:6 or (resid 7 and (name...FF67 - 7467 - 74
2472GLUGLUARGARG(chain F and (resseq 2:6 or (resid 7 and (name...FF76 - 9776 - 97
2482TYRTYRGLNGLN(chain F and (resseq 2:6 or (resid 7 and (name...FF99 - 14499 - 144
2492LYSLYSTHRTHR(chain F and (resseq 2:6 or (resid 7 and (name...FF146 - 190146 - 190
2502PROPROGLNGLN(chain F and (resseq 2:6 or (resid 7 and (name...FF193 - 218193 - 218
2512ASPASPGLNGLN(chain F and (resseq 2:6 or (resid 7 and (name...FF220 - 255220 - 255
2522TYRTYRLEULEU(chain F and (resseq 2:6 or (resid 7 and (name...FF257 - 272257 - 272
3532SERSERTHRTHR(chain K and (resseq 2:6 or (resid 7 and (name...KK2 - 102 - 10
3542SERSERTRPTRP(chain K and (resseq 2:6 or (resid 7 and (name...KK13 - 6013 - 60
3552ARGARGGLNGLN(chain K and (resseq 2:6 or (resid 7 and (name...KK62 - 6562 - 65
3562PHEPHETYRTYR(chain K and (resseq 2:6 or (resid 7 and (name...KK67 - 7467 - 74
3572GLUGLUARGARG(chain K and (resseq 2:6 or (resid 7 and (name...KK76 - 9776 - 97
3582TYRTYRGLNGLN(chain K and (resseq 2:6 or (resid 7 and (name...KK99 - 14499 - 144
3592LYSLYSTHRTHR(chain K and (resseq 2:6 or (resid 7 and (name...KK146 - 190146 - 190
3602PROPROGLNGLN(chain K and (resseq 2:6 or (resid 7 and (name...KK193 - 218193 - 218
3612ASPASPGLNGLN(chain K and (resseq 2:6 or (resid 7 and (name...KK220 - 255220 - 255
3622TYRTYRLEULEU(chain K and (resseq 2:6 or (resid 7 and (name...KK257 - 272257 - 272
4632SERSERTHRTHR(chain P and (resseq 2:6 or (resid 7 and (name...PP2 - 102 - 10
4642SERSERTRPTRP(chain P and (resseq 2:6 or (resid 7 and (name...PP13 - 6013 - 60
4652ARGARGGLNGLN(chain P and (resseq 2:6 or (resid 7 and (name...PP62 - 6562 - 65
4662PHEPHETYRTYR(chain P and (resseq 2:6 or (resid 7 and (name...PP67 - 7467 - 74
4672GLUGLUARGARG(chain P and (resseq 2:6 or (resid 7 and (name...PP76 - 9776 - 97
4682TYRTYRGLNGLN(chain P and (resseq 2:6 or (resid 7 and (name...PP99 - 14499 - 144
4692LYSLYSTHRTHR(chain P and (resseq 2:6 or (resid 7 and (name...PP146 - 190146 - 190
4702PROPROGLNGLN(chain P and (resseq 2:6 or (resid 7 and (name...PP193 - 218193 - 218
4712ASPASPGLNGLN(chain P and (resseq 2:6 or (resid 7 and (name...PP220 - 255220 - 255
4722TYRTYRLEULEU(chain P and (resseq 2:6 or (resid 7 and (name...PP257 - 272257 - 272
1733ILEILEGLNGLN(chain B and (resseq 1:2 or resseq 4:15 or resseq...BB1 - 22 - 3
1743THRTHRPROPRO(chain B and (resseq 1:2 or resseq 4:15 or resseq...BB4 - 145 - 15
1753ASNASNTHRTHR(chain B and (resseq 1:2 or resseq 4:15 or resseq...BB17 - 7318 - 74
1763ASPASPASPASP(chain B and (resseq 1:2 or resseq 4:15 or resseq...BB76 - 9677 - 97
1773ASPASPMETMET(chain B and (resseq 1:2 or resseq 4:15 or resseq...BB98 - 9999 - 100
2783ILEILEGLNGLN(chain G and (resseq 1:2 or resseq 4:15 or resseq...GG1 - 22 - 3
2793THRTHRPROPRO(chain G and (resseq 1:2 or resseq 4:15 or resseq...GG4 - 145 - 15
2803ASNASNTHRTHR(chain G and (resseq 1:2 or resseq 4:15 or resseq...GG17 - 7318 - 74
2813ASPASPASPASP(chain G and (resseq 1:2 or resseq 4:15 or resseq...GG76 - 9677 - 97
2823ASPASPMETMET(chain G and (resseq 1:2 or resseq 4:15 or resseq...GG98 - 9999 - 100
3833ILEILEGLNGLN(chain L and (resseq 1:2 or resseq 4:15 or resseq...LL1 - 22 - 3
3843THRTHRPROPRO(chain L and (resseq 1:2 or resseq 4:15 or resseq...LL4 - 145 - 15
3853ASNASNTHRTHR(chain L and (resseq 1:2 or resseq 4:15 or resseq...LL17 - 7318 - 74
3863ASPASPASPASP(chain L and (resseq 1:2 or resseq 4:15 or resseq...LL76 - 9677 - 97
3873ASPASPMETMET(chain L and (resseq 1:2 or resseq 4:15 or resseq...LL98 - 9999 - 100
4883ILEILEGLNGLN(chain Q and (resseq 1:2 or resseq 4:15 or resseq...QQ1 - 22 - 3
4893THRTHRPROPRO(chain Q and (resseq 1:2 or resseq 4:15 or resseq...QQ4 - 145 - 15
4903ASNASNTHRTHR(chain Q and (resseq 1:2 or resseq 4:15 or resseq...QQ17 - 7318 - 74
4913ASPASPASPASP(chain Q and (resseq 1:2 or resseq 4:15 or resseq...QQ76 - 9677 - 97
4923ASPASPMETMET(chain Q and (resseq 1:2 or resseq 4:15 or resseq...QQ98 - 9999 - 100
1934GLYGLYHISHIS(chain J and (resseq 3:9 or (resid 10 and (name...JJ3 - 291 - 27
1944VALVALPROPRO(chain J and (resseq 3:9 or (resid 10 and (name...JJ37 - 5028 - 41
1954PHEPHEASNASN(chain J and (resseq 3:9 or (resid 10 and (name...JJ52 - 5843 - 49
1964GLUGLUPROPRO(chain J and (resseq 3:9 or (resid 10 and (name...JJ63 - 8150 - 68
1974GLUGLULEULEU(chain J and (resseq 3:9 or (resid 10 and (name...JJ83 - 10369 - 89
1984ALAALAVALVAL(chain J and (resseq 3:9 or (resid 10 and (name...JJ105 - 18291 - 168
1994THRTHRASPASP(chain J and (resseq 3:9 or (resid 10 and (name...JJ184 - 256170 - 242
21004GLYGLYHISHIS(chain E and (resseq 3:9 or (resid 10 and (name...EE3 - 291 - 27
21014VALVALPROPRO(chain E and (resseq 3:9 or (resid 10 and (name...EE37 - 5028 - 41
21024PHEPHEASNASN(chain E and (resseq 3:9 or (resid 10 and (name...EE52 - 5843 - 49
21034GLUGLUPROPRO(chain E and (resseq 3:9 or (resid 10 and (name...EE63 - 8150 - 68
21044GLUGLULEULEU(chain E and (resseq 3:9 or (resid 10 and (name...EE83 - 10369 - 89
21054ALAALAVALVAL(chain E and (resseq 3:9 or (resid 10 and (name...EE105 - 18291 - 168
21064THRTHRASPASP(chain E and (resseq 3:9 or (resid 10 and (name...EE184 - 256170 - 242
31074GLYGLYHISHIS(chain O and (resseq 3:9 or (resid 10 and (name...OO3 - 291 - 27
31084VALVALPROPRO(chain O and (resseq 3:9 or (resid 10 and (name...OO37 - 5028 - 41
31094PHEPHEASNASN(chain O and (resseq 3:9 or (resid 10 and (name...OO52 - 5843 - 49
31104GLUGLUPROPRO(chain O and (resseq 3:9 or (resid 10 and (name...OO63 - 8150 - 68
31114GLUGLULEULEU(chain O and (resseq 3:9 or (resid 10 and (name...OO83 - 10369 - 89
31124ALAALAVALVAL(chain O and (resseq 3:9 or (resid 10 and (name...OO105 - 18291 - 168
31134THRTHRASPASP(chain O and (resseq 3:9 or (resid 10 and (name...OO184 - 256170 - 242
41144GLYGLYHISHIS(chain T and (resseq 3:9 or (resid 10 and (name...TT3 - 291 - 27
41154VALVALPROPRO(chain T and (resseq 3:9 or (resid 10 and (name...TT37 - 5028 - 41
41164PHEPHEASNASN(chain T and (resseq 3:9 or (resid 10 and (name...TT52 - 5843 - 49
41174GLUGLUPROPRO(chain T and (resseq 3:9 or (resid 10 and (name...TT63 - 8150 - 68
41184GLUGLULEULEU(chain T and (resseq 3:9 or (resid 10 and (name...TT83 - 10369 - 89
41194ALAALAVALVAL(chain T and (resseq 3:9 or (resid 10 and (name...TT105 - 18291 - 168
41204THRTHRASPASP(chain T and (resseq 3:9 or (resid 10 and (name...TT184 - 256170 - 242
11215LEULEUMETMETchain CCC1 - 91 - 9
21225LEULEUMETMETchain HHH1 - 91 - 9
31235LEULEUMETMETchain MMM1 - 91 - 9
41245LEULEUMETMETchain RRR1 - 91 - 9

NCS ensembles :
ID
1
2
3
4
5

-
Components

-
Protein , 4 types, 16 molecules AFKPBGLQDINSEJOT

#1: Protein
MHC class I antigen


Mass: 31940.246 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Strain: B*35:01 / Gene: HLA-B / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: F4NBT2
#2: Protein
Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 4 / Mutation: M1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P61769
#4: Protein
D1 TCR alpha chain


Mass: 21807.949 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAV1*01 / Production host: Escherichia coli BL21(DE3) (bacteria)
#5: Protein
D1 TCR beta chain


Mass: 27202.172 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBV7-8*01 / Production host: Escherichia coli BL21(DE3) (bacteria)

-
Protein/peptide , 1 types, 4 molecules CHMR

#3: Protein/peptide
LEU-PRO-PHE-GLU-LYS-SER-THR-ILE-MET


Mass: 1066.291 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Influenza A virus

-
Non-polymers , 2 types, 774 molecules

#6: Chemical
ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: PO4
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 770 / Source method: isolated from a natural source / Formula: H2O

-
Details

Has ligand of interestN
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 2.48 Å3/Da / Density % sol: 50.31 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG

-
Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jan 1, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 2.4→50 Å / Num. obs: 147969 / % possible obs: 100 % / Redundancy: 2 % / Biso Wilson estimate: 36.3548140718 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.044 / Net I/σ(I): 12.6
Reflection shellResolution: 2.4→2.49 Å / Rmerge(I) obs: 0.3912 / Num. unique obs: 14575 / CC1/2: 0.784

-
Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.40000208775→49.0643676691 Å / SU ML: 0.321790927904 / Cross valid method: FREE R-VALUE / σ(F): 1.96715742794 / Phase error: 27.3036807468
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflectionSelection details
Rfree0.252287726965 7336 4.96074546426 %0.05
Rwork0.218166072864 140545 --
obs0.219887878441 147881 99.9101436351 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 68.2822005343 Å2
Refinement stepCycle: LAST / Resolution: 2.40000208775→49.0643676691 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms26378 0 20 770 27168
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.010643865000927390
X-RAY DIFFRACTIONf_angle_d1.5443769275237268
X-RAY DIFFRACTIONf_chiral_restr0.07407426617363874
X-RAY DIFFRACTIONf_plane_restr0.005697491002194940
X-RAY DIFFRACTIONf_dihedral_angle_d22.984799821410132
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.401-2.42730.3611074666212530.2999363034734584X-RAY DIFFRACTION99.9380165289
2.4273-2.45580.3276394018512170.2931123431924639X-RAY DIFFRACTION100
2.4558-2.48580.3249406203092350.2847257275564647X-RAY DIFFRACTION100
2.4858-2.51720.35206153192110.2842456338584682X-RAY DIFFRACTION99.9795668165
2.5172-2.55040.3541758163292530.2936156289474681X-RAY DIFFRACTION99.9797365755
2.5504-2.58530.3259340766812280.2779946691954562X-RAY DIFFRACTION99.9374087211
2.5853-2.62220.2833985797192480.2644658547754674X-RAY DIFFRACTION99.8579833638
2.6222-2.66140.3082052740392550.2536954764644595X-RAY DIFFRACTION99.8353231783
2.6614-2.7030.2674064812032470.2513699328914643X-RAY DIFFRACTION99.8774509804
2.703-2.74730.2984888055622130.2531134290674696X-RAY DIFFRACTION99.9796334012
2.7473-2.79460.2889893615752210.2589452290914668X-RAY DIFFRACTION99.9795501022
2.7946-2.84540.3464358124992550.2509093226754634X-RAY DIFFRACTION99.9386753884
2.8454-2.90020.3009290748322250.2519932561994671X-RAY DIFFRACTION99.9795793343
2.9002-2.95940.2995485427482440.2471651893474647X-RAY DIFFRACTION99.959125281
2.9594-3.02370.2880466416782390.2466949650994687X-RAY DIFFRACTION100
3.0237-3.0940.2711353463162950.2343978904674580X-RAY DIFFRACTION99.9589911831
3.094-3.17140.2717465202992360.233307889564719X-RAY DIFFRACTION99.9798224374
3.1714-3.25710.2836767519672260.2380210662334665X-RAY DIFFRACTION99.9795584628
3.2571-3.35290.282187520682570.2332879808964633X-RAY DIFFRACTION99.9591169256
3.3529-3.46110.288241416362270.2203090825144711X-RAY DIFFRACTION100
3.4611-3.58480.2502294430042600.2174337927394699X-RAY DIFFRACTION99.9798387097
3.5848-3.72830.2296329524782570.2066968394684662X-RAY DIFFRACTION99.939049167
3.7283-3.89790.2219487014832700.201293008674677X-RAY DIFFRACTION99.9797898141
3.8979-4.10330.2336625160012530.192827789884716X-RAY DIFFRACTION99.9597666465
4.1033-4.36020.220678043932070.1788386851434747X-RAY DIFFRACTION99.9596448749
4.3602-4.69670.1830220429912680.1694323088074726X-RAY DIFFRACTION99.9399639784
4.6967-5.16880.2030529008732520.1640577597034737X-RAY DIFFRACTION99.9799599198
5.1688-5.91570.2051418057192530.1887793051774769X-RAY DIFFRACTION100
5.9157-7.44890.2480474462212620.2124191209014823X-RAY DIFFRACTION99.9410377358
7.4489-49.050.2029099729782690.1957887404564971X-RAY DIFFRACTION98.6074520135
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.798324025653.176096618030.05908661963277.74941456115-0.3498302196031.44604838640.226993056989-0.2852513384430.3400115427020.205928523651-0.155400587803-0.0903747337824-0.03400032365220.261591618034-0.1180468796640.302101337260.0303141589035-0.04613023229820.280826229995-0.05086512373840.523100130535-14.4575027364-9.7094281302117.6501039235
20.2700509069520.929519504011-0.2157176186523.27072493982-0.6726668902761.97104589061-0.208440064445-0.461783067610.1158884323240.2533786523730.158842342616-0.9823903914110.3931254516520.676419702361-0.04454681977050.3762643842070.103659045021-0.114753520640.416869492481-0.03519208041210.717931179002-8.80129687251-20.037728380117.5085009126
32.13334938106-1.19481467695-0.03336863629272.60449852787-0.5027079765111.489468748410.1126319072370.2777251746230.20705383355-0.265855157952-0.356234449185-0.1110385758550.01385111023040.1197986814860.1635109821140.230233307527-0.0304582570184-0.0003546491121040.274699297606-0.02490541654440.500421260145-12.3473167436-8.756641855117.55000596773
42.509134992991.199365882160.1507658084484.881485506520.6475841440161.195102852260.0577077672213-0.2398900000450.06693184183240.2508964342540.0307113480649-0.364284966153-0.0442724030695-0.144483244792-0.03961104657220.2174673888060.00828280925151-0.04251322392850.363546221568-0.0780629784050.447525971121-24.4049925444-8.8467637398115.9658915184
51.405317139840.2421271010750.3468341699341.357250563060.1440008810621.181580485580.1826347007150.01793867903850.335578298660.272996501565-0.1091164960430.1485174577230.018404379913-0.411050631266-0.02021354597190.204005704836-0.00885612393115-0.01704549867340.306104968979-0.08095244744370.5880552003-31.3317228499-4.9245471235812.7415005569
60.4714836927740.2453538727690.04123432851141.948740054181.309238480751.678562543920.1118843957880.06959026790430.234416619732-0.0628824896392-0.2955965485540.01748290716450.0176299926449-0.2709604482660.08643595653690.1931121772660.0127851199307-0.006146376491640.267269269469-0.01500262225140.494845230279-28.697818466-8.199633393094.23738371252
72.2400062677-1.814825758370.8142883506414.12480444072-3.268339661663.977132342370.009719347134660.141750790292-0.301707335606-0.2720685465830.229466477698-0.3314174726630.57754919663-0.099610320576-0.07399135576670.448998862878-0.0496588141425-0.02802993464750.225174963926-0.05822101801240.409027748848-20.8132448967-29.068411878113.277627898
81.241047945970.02766866900630.1694319717490.357070908371-0.6554136529451.261594982370.152908639039-0.5753721810760.03471571427970.249824122045-0.332277663301-0.1071488425780.1211233593270.533760809314-0.2250984211820.6946973032-0.171813294871-0.1692583216940.6184703970320.08692261576010.726378531477-20.160491996-24.867521486144.5471139057
92.17167730825-0.560034291521-1.576684174511.103637811730.2089299458912.315770351750.606420161006-0.753145189074-0.02141787888070.176520132551-0.8839051719220.160748091205-0.443699318354-0.09824205451450.1302497881240.704067613033-0.25742587052-0.04305835890830.732408215336-0.1842118922420.831471924934-28.2647851709-19.101078754247.5680975472
101.644742925540.696710259029-0.3801061253651.45180576774-0.7183498673151.852658413520.139464631766-0.753123407470.3821314842030.318445124825-0.358899365683-0.03160501059250.068227290681-0.07135487384460.1840945385520.623094649075-0.152624225939-0.0864019724140.726276195966-0.03878052425870.673143550474-25.5844773816-21.676062115547.0988691206
112.19092959453-2.259536158141.794396131325.09523247744-3.891967059544.294327347290.122381556109-0.419103278162-0.5894015552310.299186946708-0.292315567122-0.323979861258-0.148332797204-0.222301568526-0.01981027696160.602610909113-0.00217127018364-0.324008499790.524188631747-0.07096039578270.74222313399-17.1219226474-4.6514975183436.3887204971
120.682772421149-0.0700035194968-0.03316846275441.851456591090.9241177666090.486104005569-0.0109312161207-0.289710164618-0.08860105913080.3968181133910.00625136106153-0.183296388484-0.06043912662950.1258028859950.06232218279230.868411983448-0.0420189058755-0.3801363633960.8720872310490.4906385403941.73465120084-5.64116490015-21.77969143746.69815037
131.80578637805-1.168292198211.734500448511.03965626584-0.4432984058083.31815411205-0.107197104436-0.108037352033-0.3642221942820.540684700618-0.535549369953-0.78000363079-0.6792410800110.1606153225890.3342146971190.462816971271-0.10483339008-0.3409148181550.4698804819440.1179062178821.00202872454-10.1744638076-5.4018782647134.7969105646
143.042146630010.4489895259182.510095184221.339172744090.6255846929612.644253334230.179696789807-0.0293410822566-0.1002699050160.2595977864550.0066580110485-0.3337929455340.0139406775270.561905181168-0.1019650857470.824242804504-0.101786768776-0.4304024319721.172831545050.2744347135691.946793783281.06591592533-8.2923468890438.4807994366
150.655620375695-0.1063709688110.3680211898180.868053182851-0.4691967687492.19899214812-0.023879994918-0.379193749719-0.3445953487540.629644948314-0.524132900029-1.268816488740.2343877756-0.003408909923310.2781923411720.663487604076-0.15709785706-0.2912824066680.4555660002960.1552433747741.03163754648-10.1567222528-7.523484817236.540323507
161.5502547124-0.6705521838450.9350560659663.18127418878-1.5492855743.787418754920.00892665108514-0.4172890246830.0135354884730.211530189936-0.0378463932703-0.05221703261980.3719784055740.137021317298-0.2873701632271.29859555911-0.144831743902-0.4712133107140.8701171083080.006966387954130.580448036888-11.7369302893-9.1727453854248.9755481052
172.32446960542-0.5447788937190.2160539522851.23098434885-0.008027335934332.93318184885-0.1189534964120.2122016907990.47739913-0.00997312457863-0.151213505344-0.2327855699150.295035167946-0.2616556702530.08570489122280.226877007301-0.0548014342619-0.00486343945190.23788058983-0.03519232904120.377778899786-19.6659585979-11.56588909425.70941847955
184.4538931039-1.450095515282.691402800471.57085640635-1.123237469012.72297664499-0.09670800644230.2575735564150.324289208275-0.119168516902-0.0708726983521-0.117827125320.26485014278-0.0394005288928-0.008969217436820.319246137368-0.01528116348090.001140766981440.264064919550.08987789054050.370754562243-26.9982955291-23.1917610608-23.6451158575
191.277882626730.2861218288470.24050592961.496804494810.4468344563981.130740308940.08290280164920.05523750660160.123771905694-0.0548120150657-0.179956206751-0.1158791638740.2557659224170.22960290880.08567840078630.1776201645720.00362998029316-0.009157026398340.2367529007320.08389300122820.32384321656-25.0654012217-18.0782967454-13.3942531123
201.89161411236-1.090194053920.8683674203032.59877278086-1.429258010043.12173286992-0.07717099977250.1374353157050.2461355440030.0151535756324-0.038337461303-0.2161313280050.04283658672430.0969263490698-0.04076546967050.251095877319-0.0357023316197-0.02394391153240.2210262575290.02139134697570.379212375454-33.550239944-20.1685642473-12.8326974599
211.18765316152-0.7328526002090.653924118871.15057722451-0.2056231000511.655126850960.01855589385560.1289696311020.405912529451-0.1071573450640.16321278872-0.153337275337-0.140938773221-0.4718464719890.09779482270680.273164598036-0.0355228375575-0.02715400009270.2984986150780.1796633917430.321459687127-28.2071340636-15.335482961-22.4322226308
220.5959679721110.185860662471-0.1398047595851.307806047060.9997964399362.46335332157-0.2871578796810.0441220546410.05743180840050.07862540769420.0470239711985-0.202185258982-0.121997389090.0309266654982-0.01141800274190.171877888381-0.000923087842322-0.0396289851630.2953261939490.0708330964360.303361987792-16.7472595757-18.3635051731-10.1870179616
230.503226546614-0.008790604881481.581406896660.659332156919-0.3925944499265.20373498388-0.3226909807160.832843023241-0.265439342861-0.3533412268850.260152859686-0.191307920159-0.329598902083-0.1292661061620.1383987872130.617621620768-0.05191561634720.003795565568310.8103027107690.2019729053610.403911124873-33.0581428261-16.7002205025-39.9982780221
240.7292889921190.3405641000790.3629585719741.13632590457-0.03752677078730.635766283956-0.07778625283070.3377759545050.22074950914-0.446942795680.0614574115041-0.271367552708-0.4466619553820.3197844668470.02471138890221.18628197443-0.09653330483570.1096971939871.85229870153-0.1134646191521.16072892226-19.7314022342-8.50937952204-55.4802212104
251.47031029671-0.7332578878250.7501180380141.962086214370.5002547332160.8577087683690.1229335688930.336150901737-0.233513021888-0.06370872430110.37721973189-0.2862569828410.2662048867110.519577168421-0.02595179863610.9877366145490.04741876519170.2663030735641.36018765995-0.003830363009521.31339634284-18.5388319891-21.8688281634-48.6958634885
266.1547956811-3.62079239658-2.240665064555.295368681091.503060151973.11190116906-0.3816624623050.120337395925-0.0732393199963-0.3284404267730.2219892370350.287327612858-0.0253721017287-0.03082502113580.03052263957850.709834867044-0.08680201993350.05872191658861.148064169530.2982176819580.685083501939-29.0582572569-8.72504472441-41.8500860601
272.836297681720.148921639541-0.103788949560.0570372286337-0.02272126508310.0265777079884-0.2643788649190.383798485809-0.0453858629109-0.03867830455110.134061958058-0.00990495889544-0.1892435618740.575564137460.04815183549260.9254775500670.06323494218470.08308770393631.134017571580.04738041782150.530392884045-24.0399985445-11.8582082686-47.3911586007
282.083492651241.2756022936-0.4991803634530.833124591321-0.2110291433520.261337099064-0.01520299859360.143253216049-0.225099354656-0.161513307897-0.11951840395-0.1016434124850.1732865165910.2787451339770.06169870442991.18535729296-0.01957095440730.1262633965621.7166831212-0.5214444680421.45191093468-22.1799069317-18.6210354017-58.2666931042
291.369743006460.194312077655-0.309931369740.756759052733-0.239636796691.466960905470.009612875960340.1591506145860.335822807659-0.126924021483-0.0549690165266-0.325327637250.03710122116060.1925698842110.03827138240910.25003502874-0.02266146546280.04416308989610.3201837158950.1296412359740.64418443929-5.84751320214-7.63735588226-15.9925926911
301.998754016170.5670181404570.5092738070870.7627551764390.4544380565941.65886168325-0.4081881980930.9054719232080.772543233684-0.4975195830430.1998609208440.0427270070191-0.384728656059-0.0811970886451-0.007217961531110.671567154689-0.0312198386540.001725990172221.014467869310.3868778405170.740140472112-14.1784273267-2.14497856869-44.2781920701
312.74192706875-3.872192499270.2714505665687.50017045232-0.7049446256611.49650938580.02566594710430.304997430910.0408802390672-0.5995989002830.00298377550652-0.216156865714-0.2008674896580.3998449805820.07436890315990.284600467074-0.0932835707132-0.03155551761170.2852298069250.1207798781340.50475200131424.392125505710.1104218453-15.3845271143
320.128742122945-0.2434640115230.2406116254913.24334609674-0.6402187786750.90849595038-0.1056751433690.4531461365470.286465619848-0.24597890972-0.00662275503645-0.648606090725-0.471639478040.534026684229-0.1545526409090.535027819156-0.2113835456820.01176583649290.438904688760.1792311064450.89667970644230.014963356320.4361253604-15.5964024579
332.624910232791.542825647150.02963869545283.08541461269-0.3633849765591.2731219857-0.0664862157242-0.08057920511230.1913460467910.3030780814550.00435426923024-0.277267698947-0.2476944542080.1211163284840.1310197076510.274817228225-0.0278383277092-0.02401485358970.2804189606870.1053517211250.4100556995526.88959968559.1803427442-5.43077374881
341.299249301620.376471158047-0.1883281055721.009516665810.690578035141.35349107875-0.05905225557310.1030930080170.28407966412-0.1838114108480.04105831708970.225314683335-0.173915570552-0.03921152447170.009352129583690.2446107838450.00122991914448-0.05329262771650.271446642180.07853743190530.38624780028412.2055514067.95637444523-12.2208956789
351.05247439868-0.130800857998-0.03613168182.002622099911.082164231581.81290427438-0.0662928843453-0.22431595980.2260371944490.159427720585-0.08580939384020.1281502205740.0187741238271-0.08607971698120.07416700790240.231910358794-0.0134888112958-0.04361242845810.2363158614860.04040510607080.38810442178410.64675381158.59844934636-1.5550409789
361.731336938261.12963418172-0.6874715556363.64236680554-3.006365853742.87399972636-0.0619363412796-0.07660766024780.3214479139670.1661126079040.0432989923321-0.298960099949-0.8325175407870.110597473492-0.06920147230660.6860685407630.00766414390592-0.1786102650330.1547212244470.1150601773010.75660034794118.166063908429.4596513475-10.8904677855
371.78333804681-0.814734938066-0.1496859229860.886502418642-0.1994135523122.670356615620.1966307536780.6477348015420.216382657624-0.264005562576-0.656230856635-0.206249066196-0.3238627509560.004241316339460.167729840810.7041882549880.101744509433-0.003619675658820.6677656703660.2148509730490.47460365038514.606998506522.9572372481-42.9268150773
381.99215069016-1.034788559750.3010984426162.02474970712-0.4759672485662.335164332870.2995230207670.731167236006-0.242732280055-0.182474490214-0.418042408620.413203906026-0.268670163744-0.021386942740.09823803210240.5974566547910.131313751827-0.08260160048820.791795265840.1015209830480.48055840077910.185798390920.9111655883-44.9854193745
391.861873302713.20729353226-2.564486742928.38697619427-6.308514066745.75106679938-0.04344038868110.4622337189980.05334598740230.00673584678681-0.1386859319350.02930914523790.1755596006960.161017953340.1368599937540.46712847425-0.01807756436060.09808446034370.5012474755380.08578541340690.5305665692821.00776913144.81109409757-34.1151117242
404.74272826014-0.781777045985-2.669954662731.8804933730.6506252773681.526667191240.1042315673490.460161318967-0.00680424018049-0.0613142456199-0.04088628328810.0135070145012-0.06216870746420.1570392080330.04237056920161.0697923658-0.010454608720.2587286579530.8993294258080.3991705663730.77424614542631.834106192322.0553053437-46.0806471084
411.508784356341.24312648453-1.198052155091.02907056546-0.9735935617671.79420323077-0.007740998168130.3467549667740.400620931803-0.511306243169-0.319558385193-0.2928967067590.5051480960790.1569105498640.05292038611710.4675511796630.07369245271940.1250441133920.4854872341770.2413425883410.49683081113928.292667616.50436000037-33.2716922957
421.77565518146-1.76553495987-3.636587459691.996540836982.974384554139.13976895437-0.0241708756165-0.1508335030910.09873207304030.175379143417-0.198917943915-0.535105465532-0.02930859009140.901659629645-0.1921927879090.655535259028-0.08220315329860.2452721832270.9461709632420.4445089192561.1245129497738.8835205488.63536479611-37.3475786764
431.253287946850.718852817307-0.8314686073421.37955574387-0.7126531912981.61299732746-0.0006535212325810.3548809887210.350231224522-0.482304495965-0.179151496279-0.591323250812-0.212435429720.4653483487370.06134581144590.6055934143550.02969347598750.07697431868560.5559918129480.3186878618650.56627390990827.9161080237.64047218467-34.7869964646
442.41542679486-0.6195588263210.8645862979636.15775905332-1.919573477552.944033481130.03491017300350.5829676464440.115818323188-0.3096256233880.01490664112990.04420997959830.013794691369-0.159541847931-0.02741554429470.8110808033660.2219856003050.1110468270470.7600700781210.2551946670770.40966264266625.84165412999.24673131697-46.8458946136
452.150970862610.0389066856842-0.1123585299410.009960729733460.1325554072642.62248508173-0.268332005916-0.054794074888-0.1486091000130.10864452576-0.10362657897-0.325488335551-0.3507904028810.1765480351790.1837825005140.250001737177-0.00817536087307-0.02066031532160.320037768740.05835106131940.47388419151919.694976218811.9353691934-3.34530739937
465.555332444391.29061608433-2.7257486362.61950193041-0.7021448848664.67266949765-0.174859656898-0.2174276025360.1348299815870.08132319559020.192726469782-0.0762310219121-0.3765198040010.141554105775-0.006512681711420.3062456468450.0173242271510.01983542877060.279526681324-0.1267358711040.64859114331613.369300934923.749331363926.0721228717
471.34048955104-0.4243505016390.2881248542621.84175183660.06797315744932.337945931-0.06628860123230.01783787110020.5795704809660.0859492643722-0.271314032738-0.135116830964-0.3470799483310.2948119302030.2069067082780.235285535294-0.0235215372491-0.04563317882560.264307898077-0.01743317772560.51120437578414.955916274418.585779903115.8002521837
482.026366628190.934054043473-1.017812289683.21762782459-1.685953179633.781452773390.0965298621954-0.04897912988640.0991860614143-0.08699103847910.02230918057960.198514330338-0.2719433474080.235045916453-0.07552802303880.2903795899810.00744016514618-0.05334665272490.25373865462-0.02581841594250.5691387449956.4908065489820.632697381515.4983693415
492.383795479320.617305641078-1.46807894871.48154477285-0.8139996470883.19637312911-0.190956118951-0.1632167577930.201823033-0.06055853113080.177248329969-0.08161162207810.271901834348-0.0454200852601-0.0105162699340.2353922862710.0188804874592-0.04524011000160.215303938297-0.06497153033070.36193318743912.103104238715.84598494124.9746670856
500.475205492515-0.159820217905-0.004450847400581.325217850451.086624289762.60805350927-0.326053979693-0.0436040519370.246805424401-0.1669491168010.05172540043620.1471699945140.01290154998310.2800892304490.01367372567930.23284445796-0.0199051920222-0.03442970024820.323939589815-0.0152660145350.55855594329923.199942029318.795697787512.3922705414
510.2340000731670.326702053252-1.189202553470.884658883363-1.467548738736.40342207078-0.0250504830536-0.943359265589-0.215161509768-0.006708164153590.2775168601110.1933120966540.1695160723220.0334667533409-0.008457482670280.4543873319520.11243870645-0.02343294593170.8335395834650.07613378627420.4485359866417.7770664171617.032042482942.6661329881
520.9042508095930.252688295341-1.024835815142.014753025090.4290522882741.426699661410.00306738127004-0.191803225627-0.4029664303230.1651206740240.0180924401963-0.568047810830.3969404004510.3591055322610.009995217555891.364600686170.091555759312-0.2454591269372.37293727274-0.04022884124331.043284346124.42779751085.7025995888759.9897617875
533.01148083495-0.415824664646-0.2269333271974.1932641274-1.608707635782.79469158821-0.09232744986610.001549277510130.5338814245070.1794669039560.0532499802793-0.255134712741-0.2035118790420.118117937461-0.01810765772161.059039650780.323193972519-0.04058300243351.67040481353-0.2882971283841.2001540013317.005806199318.804558855951.5862303866
543.462210059351.017053156970.7372092832743.023434404620.3678464522542.62045188187-0.213751212703-0.7439623437640.02689807955930.1899606169420.5740878081320.3524530148380.2999145933670.468053089933-0.1895812015450.6215024028770.166701330760.02761423567011.31902330283-0.1033641367150.75766925645617.292236200912.012722197448.1707380705
550.796882884007-0.457639758297-0.3861202289950.661180677167-0.03247767287060.3541277322090.0386579259672-0.05681288981140.2850862547180.09221002761390.008170160720810.451688624067-0.1152935135530.238975351440.00528944740491.513345256970.233116601829-0.1009233686622.2756535442-0.5856240585961.1879622711219.156313177618.624261815260.8122102511
561.71548604382-0.2342069782580.2111932991351.277680364750.2637490877431.69772135027-0.0850029548747-0.1522574910030.2815726915660.09455872769110.12901403953-0.248756795479-0.04409082839380.237086069241-0.0449154591860.2399529272110.0053799118633-0.04527396540190.273202245519-0.04658196295720.41061434309834.17014203078.0597593716218.170749807
571.39092464289-0.100777873246-1.028875334570.6615483473250.2464991769780.8124426295670.141578226587-0.861369120744-0.2807801185960.5192074944070.3942334114080.3651167827160.282119511542-0.486072253473-0.1729535290520.6968427515020.07145768454390.00335117836031.352321708760.2275137647950.55124201957722.3538615623.8704574203249.5420618423
582.141032686210.201258298042-0.04537363651653.26537230661-0.009607216542262.38552729290.00190061212126-0.188569240698-0.09433125778640.001027594567590.4315739150010.263719090190.06164647854410.338686083264-0.2120137655090.851217193750.08033374401590.001274733483980.904387946847-0.001075325313960.52170533715627.73372754539.4232974822442.8303619867
590.7757227077010.2697250944040.4978363618261.446458404970.06483194268181.387019637660.0616076741158-0.960461220106-0.8839125373120.4801456884220.2129215320810.2433976594080.2675901412710.0552174517096-0.3083499008190.7119031818150.146400480902-0.05409461111420.9076850765510.2650980520720.69427852208828.7990262962-2.1155404379846.2092150058
602.458684552512.170013830890.3089372908496.472568268320.7028898480591.458467231770.191500954103-0.39645456611-0.04347627930010.123713886743-0.1230931520130.123038634854-0.0683541355854-0.282039565786-0.03809332519050.1957077116730.01266536596340.0449881387570.252467690338-0.006212009205140.085487665771317.2289704699-38.77921625717.0545625286
611.307241437580.008206033043960.2795814009782.833168655750.1945303621830.951812643033-0.153006373824-0.4329700466580.234392408467-0.000598800680701-0.1942836291430.720117929477-0.483962951569-0.5001175373690.1005880241330.4558197157240.1084904408790.09954087672530.335973327962-0.1163148106850.42436103825611.7034061699-28.17412137417.6331291974
622.27383958089-1.21837969510.1540750332963.350013860950.345870764741.38433283192-0.129077233768-0.01033536064130.129429175614-0.3143946216030.1508472192070.0587599742607-0.216079144283-0.07825693865380.08048794902490.220099973771-0.00993425945668-0.02105279298170.232570208748-0.001329437248810.10944422436514.9996750399-38.85596009887.04321913778
631.71939897342-0.289803387339-0.548817500691.8687572087-0.3691183327911.26943829496-0.0056126008019-0.06502687876060.01981853687660.2954920265970.0537217585504-0.340405306433-0.157948170080.1575866441050.02724930266740.218760435621-0.0178900474121-0.0128034897970.2148859075820.002258708634520.023622094376929.6032189827-40.47002206813.9517354189
640.665360208981-0.247522197461-0.109762795390.952905060967-0.6734457265821.236585602230.04050215836370.00255465728911-0.0116739413933-0.0373268088126-0.0306921624931-0.172676977999-0.06937043487290.136716907848-0.03658423294460.153871034333-0.00798750204109-0.01945549146530.240082534137-0.01276717614560.1349954778931.3680635569-39.30098449753.43709606915
653.44245866559-1.440454624-0.5574516368962.927147836473.626516212675.050661035870.09078042391730.2038931874880.730760210101-0.177644817645-0.2684777118990.0239843094191-1.00042133464-0.153586795325-0.1577005175180.52002517075-0.0121007920386-0.0326006995640.213026497823-0.08769315040230.43306995853223.6433530277-18.909593031713.642218112
661.380373855450.1926025765140.4486961696420.471751573542-0.7334492491883.05940723380.0333409011695-0.4725761594620.1969234213890.144151367778-0.223126957570.1793710733650.115434157145-0.1582219085250.08017915608480.778567173771-0.04006854352780.03378908118770.551310077979-0.2048540108490.40253667659326.0138694396-27.337819280145.1169014614
670.7651176069250.2222585088780.8425517779810.783544774929-0.3312894794752.8427308654-0.119221164417-0.07397433442360.04417970069550.167533462723-0.125169283656-0.2231932595170.1355286370830.202024549896-0.3493614985680.643852727218-0.008360353407590.02051205346780.745699445951-0.3330859456850.19060875351329.9988520711-32.082439546547.7697505905
680.239935656793-0.248243417330.5830612537970.286681697985-0.3426819091723.86282021787-0.260326693605-0.1467551392190.3252664962050.204774270177-0.0471303142736-0.148423662655-0.2325646639710.4569297456640.06402934250370.921212814544-0.3288761171920.07549335719090.793962676187-0.2135484749070.51738130568931.0519755003-20.776168906346.8605970577
691.61868906921-3.07209059375-1.628286169337.907125842095.758776160875.082677880210.280400431041-0.5994522329460.002710545236580.243535952302-0.1146139981420.1031698201880.2635365164150.2280504751230.05281436125940.544695483813-0.03353581515170.1532247435370.5388774885790.03424313625660.27170114609419.7999613827-44.343127250835.2322608749
701.252760466430.488402373025-1.100629052590.191541106088-0.4298387864760.9650365237550.156465708615-0.2460431205610.2472572455750.113121892009-0.09767144015360.0670213200205-0.213333362148-0.1378764437340.05195904401440.907154695927-0.09192087777810.596053046061.01617670864-0.8275205524071.18072985029.16765861664-26.5284068147.0184371203
711.47771857410.575786498231-0.7730334550920.9116152792051.101092013713.27039543630.109811108068-0.4424832694240.570233081189-0.0280068819257-0.3731062414750.603387428593-0.334871531639-0.350254183782-0.4567103154330.554585661414-0.03247307330160.2444944682050.463196902688-0.2328088672830.39375691814413.4098457323-36.319254564136.653197776
723.23995261236-1.65900869482-4.196302340611.537049526482.77880312786.27598466065-0.169462012919-0.232064213911-0.1198350563670.404225912097-0.3427932100050.3423481389340.385794610851-0.269236519813-0.09309896461930.519447068235-0.1652311921440.3472285137490.576059302282-0.1575225525010.331930675410.5945687765-45.782700325232.4934248985
731.706575999761.39860248841-3.345150613281.15071621628-2.749931049816.557938590790.125650900913-0.2249839593960.230261198880.2951474885420.06117904438740.673317050570.342783030055-0.853861785107-0.1987250384360.725974927541-0.06965604926830.3122331134020.990442042982-0.2612805083840.8482753724191.70730781614-39.97811805137.0185243572
740.7372568651490.471523048457-0.2788694624832.48584071260.1731626120051.78145278820.322045349485-0.4573735927530.3271035346420.4987423681180.03684989320870.667125861427-0.194334239861-0.3297803320611.201375234480.464321490592-0.03083650361620.189865853853-0.0489444224157-0.810381869233-0.39229466720615.9384039895-37.544124609529.5780331825
751.42340198517-0.430178355786-1.273967132870.8825913907931.172560536692.475764881020.142345048206-0.6577806709130.3518639822520.529124471112-0.237907813710.4266803404110.08401787413180.0275314092361-0.2273433723281.0373377459-0.2884415603930.574419683440.769885789687-0.2505345538210.3315987488689.39164344098-45.617482987942.3170006441
760.7404103931040.4413770226140.7493041899333.329815266741.884169056263.18312251073-0.149073170879-0.294764457446-0.01594909543620.0396352802787-0.06804012984450.04907530667760.0704777902364-0.0230398232566-0.2572644637171.0549569835-0.149541432880.338896826330.790175563967-0.1102762935880.31021847614113.9653480595-40.257467782947.6682484163
771.166878883750.2598245439340.006598213340160.781620501927-0.05169102143171.57291938192-0.07581664546340.133772940691-0.04706351737240.0794146660418-0.1012484757350.237865396025-0.2001029866630.1615191045520.06801684958240.227769791207-0.0313279141470.01161781355490.2422837688240.02016041810310.10119171932422.297489781-35.98666955755.18956650066
785.03268984554-1.29138639952-2.647489504781.716168367561.562862943813.20175804088-0.1470598240540.1705950361130.402559102599-0.385110865359-0.123143847544-0.167148631696-0.02157487315870.094469520560.06464231787210.317273105539-0.03806735273680.03899042684110.2676482441560.1217215266520.2734475203629.2793127812-23.0390177371-23.6276356614
791.164770371710.3373652949410.1314005828061.53052218087-0.264538484641.28065899637-0.07661615665990.1881440075770.287422396105-0.2251696542430.02615093997220.0684352750425-0.0644675141682-0.1581801814350.0672227207430.1984481564940.004067523165690.01239419615140.2045060509030.03242796706230.16935254843627.4148447153-28.5829991097-13.6255403712
801.77411254993-0.827862231341-0.8469475279412.964093768481.542667233882.64900743936-0.04859005854450.07906563222440.207583521563-0.08532312328680.0687147896867-0.185627183692-0.2620862174320.1145814379740.01912335227460.1792215219340.005996162516210.008343614612930.2067370912880.03521268518980.17779921477135.8827306784-26.5055966843-12.9804902976
811.08580367997-0.04295982416530.03229643905781.048313375970.09013621014752.33057174944-0.1805081828760.09492415177740.051430717028-0.08080810164520.1570381387060.0561714245394-0.02624451674750.1069278507780.01768672997770.228765036116-0.05236567921950.008155457607260.201049731599-0.01883599086550.14735594743826.2590460042-30.0290160746-18.1292720654
820.336452601464-0.362870832233-1.340823017730.6872677546811.511140640845.43842930251-0.01309420167770.7172498434020.0302304910688-0.5077052736340.0244171557401-0.2474437906240.1395473478590.107179093340.03550874704620.444170812702-0.01084577217840.1011692304980.90062812982-0.01252445505840.262163973235.2367586052-29.5275905828-40.1800607027
832.829525335531.834000985530.04294496853464.764762082550.8752276953631.46255689522-0.1783616471180.5456303191780.0346469733898-0.495370809973-0.1122928534320.578168201530.544442996552-0.7560553858760.0757059102331.0443167158-0.1495956603070.04834756302521.67566232320.1604163742960.46635789590921.873017144-38.2315914624-55.7066025099
843.904785450361.031435013460.2249833324430.4393030951990.1098745692030.0535086264182-0.04926236010610.2475700793530.604212066401-0.08079151231040.200812671964-0.359957878457-0.386223272695-0.3768647010460.1004300839271.226316911940.247524501572-0.2366728541811.464767108260.2919978463791.122500303420.7030433392-24.7972103221-49.1813439589
853.334808792230.1305702326651.192630508211.874301153420.06692793734653.29707450428-0.01950512211660.209290546344-0.1671472624320.3473561965930.29205842787-0.1651224469030.358801038162-0.187351668802-0.1344390342210.9766960671680.0505747586960.0723147911281.215750816240.1463790240640.50029643394629.1603601773-36.4013470062-44.2367667598
861.50536767628-0.0992224703115-0.9003850089060.1984366798070.01673976100730.5459606009090.2395325629070.2793513054040.357046256344-0.1494696898590.01475210649480.0222040356575-0.415299003742-0.5578672576230.004926290527911.017213902850.2358622163630.03828647076671.602862458830.4199795045190.816665371824.5375958556-28.3137163405-58.4564634862
871.454381060110.4353051184480.8824782092450.663620895491-0.1573803630561.53290169871-0.07015061426030.09762256522610.15067847719-0.1803607455470.00912137994530.0289154527440.0343271789858-0.09578351015760.01591524380980.21603725044-0.00834777940194-0.01424874202050.2372816880640.00139190485790.1083704850778.18949866432-38.9787607913-16.6086129513
882.134506261980.80253133356-0.6334194516061.12630481120.04746772246231.48662558747-0.334191338871.19761655206-0.445341359963-0.5724683956440.327815968889-0.219436606380.360370874880.07479932084710.01195130553850.667451597827-0.03820414661430.05462408890870.926924771005-0.1604870385740.27900833238116.4011850214-44.4547157818-44.3987039094
891.47148633121-2.23146710577-1.004575947946.309006366111.115318204981.48777138413-0.08667645402240.2295240542720.481601373662-0.534456114146-0.1479127451670.360729802663-0.338298406842-0.757443133415-0.1761724351860.9021889125610.293323495599-0.3376780181110.511709525748-0.1655823472911.01422619054-23.903097481138.1741152495-13.2964745986
901.31695524967-0.584118491641-0.3575559106272.869873723460.3544559436242.26760707634-0.01007653728370.136724259128-0.179833949385-0.367179335558-0.3654178153350.4589896320040.149757933961-1.114134421570.1098637110330.6315323258270.124590155928-0.2151646053040.618716897702-0.2246367723470.914388276765-29.016404650627.6722103254-13.5638878215
911.929054929410.91447756539-0.09193170711763.831615014540.4753626166361.61456772516-0.07517364285070.1483786686440.0453873397580.290912320399-0.2081636877260.205387012367-0.404953334954-0.3225003649140.1481032442110.76785732750.20410653537-0.1384501844830.507587379538-0.0934133744960.648625555255-25.619969308338.6314062187-3.10549611684
921.8213677296-0.761116429485-0.2462019079614.367074209190.3842072325681.29398824692-0.09694988698820.269945835431-0.162250711198-0.313382770719-0.3719420089410.0640031181215-0.636995265676-0.008349277087340.1175826520330.9021553616070.181810797596-0.2711274714960.5044628271480.04137395417310.762605036279-14.021096955939.3402873504-12.0670758363
931.38214647714-0.9003417677870.8811191379311.53020489823-0.8357924191830.718911326974-0.2696986572110.200650398143-0.192563461975-0.419477473488-0.0291333976833-0.410929961949-0.713000916050.2615480223020.09147500762580.9042205400030.0761724614591-0.162065017790.5021398765690.1119757352480.80741394308-7.0718097276943.5719485959-8.98027146706
940.8656110003650.2295478648440.1612936620031.13087243766-0.7710445155051.41253717634-0.2364360415930.0265080123030.263445883108-0.225245541171-0.0190990746001-0.229913243816-0.580498019370.3274029085840.129189914060.6948249719140.0559713431272-0.1240859199210.3595740725630.01569664620170.74382895445-9.1219525062939.7933501449-0.559072023673
953.711817396561.370151874190.05443344461194.124611828772.511149887775.402764956410.07823633768640.0657823977175-0.517347365574-0.00732675369102-0.525986260580.2289469649080.612696082855-0.1101635825930.004553825539550.8738688247420.2027116560850.03311142184010.407477035231-0.03231701989750.839821545508-16.440610600119.0097967536-10.1984526289
961.591809738650.229107553766-0.207688065351.16522227491-0.2917286636742.483753407150.002577684169940.265974633569-0.01628948671020.009975620523620.0876871469149-0.0574331610706-0.1262895271860.245426186216-0.0165256053790.6819048718680.03160975337790.04144102212190.577641308122-0.02020773540140.561573311602-14.506294976729.700460128-43.2290547758
970.4888199678380.420961482496-0.8504567050450.492966147067-0.7447153661181.49136729368-0.161130175619-0.111808315807-0.0707452404485-0.3158667506030.09048479565220.07062159393950.02508396627530.4815246723520.04657101203880.9117301074810.203401340865-0.02495827416210.802448669927-0.005008645737550.717385581432-9.8801162116822.1036114446-43.763676879
981.897302160131.969407491531.573610958937.300950394284.899801811894.69689703616-0.0207377575077-0.1397847946380.1007532746760.6118653011160.194382792904-0.139547105479-0.579955553226-0.0214408469035-0.0600495627370.9759731515590.0726695953299-0.1124805236240.5812973461940.1880792950570.573860323037-22.544516489744.1800242752-31.9052035468
994.04409956796-0.5629730716671.100275656893.16529828228-0.4249144387090.323400138966-0.01263757990120.258846773685-0.254987844812-0.2272021413560.176794072798-0.03021226061290.36555281528-0.156918375406-0.07153151838350.8026956098650.06847218240140.0693511604350.8362809753020.1035845585010.641637963454-32.948219888526.278846414-43.8833043918
1000.122746784434-0.1069448385790.1441954185790.105570462739-0.2001796179480.506659823988-0.0130766280525-0.076529369208-0.2972870763040.206199911579-0.09452895215590.1554390331860.157907755295-0.1407916609520.06320311446091.018174545690.01593414959680.08091876591350.5218268503120.03912537133850.583132560359-28.634522214636.0865947592-33.2856420832
1012.106939653480.9498193705292.116723648320.5530859763021.641659429915.897664756690.1861375965450.119616384260.4809626268580.292476417985-0.267750576974-0.195244775487-0.358137757919-0.5988581747540.1073531921531.040020287280.289110017296-0.2484666411220.425591181408-0.03050148519760.601625673329-31.643448515845.3651819135-28.9440892176
1022.53689240208-0.336009224341.554276395913.36697240072-1.926071334951.861422126840.06516337556370.05059602187520.1755359453440.0699384654337-0.126042835350.187437087664-0.389525423288-0.3588953205930.03089004026250.8355309924790.0900350084368-0.05927340800670.8471708653040.2566551565450.597931896802-42.357543600342.5893632307-38.5212995138
1037.54933069706-3.004905776040.9877807776253.96132663101-2.096569827963.649186622510.02841565771670.2816714187470.110255963903-0.3849270966860.2497192028690.429154166428-0.0386687275131-0.454015821354-0.004901092082941.161974937260.139480920150.1439651299390.961520650980.2556811878460.852013589605-38.942694698336.2987387543-28.6178118216
1041.393521046471.513244585950.1835813715523.277621827910.2498320262950.346705137784-0.0704865389622-0.0272578127781-0.145873610003-0.0120374918028-0.00158091956245-0.004281691211940.05772027704360.0495218203058-0.01549456392561.207929369670.281098029525-0.06800087422990.2390306982240.08261890187110.663591337689-19.51950653239.5503882799-20.9805162229
1050.435278304494-0.612166523371-0.1408601870420.884890944255-0.1358304788353.66285075217-0.0873449812968-0.107766593539-0.3075109558830.2094824612340.08890669861510.3889948053210.288468373977-0.54000567019-0.01977703431810.8768256008740.1209086526510.01250921023560.5539745825760.0808495049840.905371077406-31.503416411135.4075177737-30.8145534097
1063.7174158039-1.789241624912.353548578591.685954942520.6971459108535.62372826305-0.1367655332620.213051594379-0.026255649323-0.4609240650750.1572075137660.3512636596980.15719583863-0.382472207694-0.09178473625180.910506549694-0.3009113785030.01433068681611.15592995823-0.0443004635060.861745911253-38.314510657535.3909355509-48.9059465901
1071.57870922050.8449508532552.067226291521.906425200372.333097112493.72567837145-0.2006729481890.01145092135040.2097171801870.00653420166919-0.145868840383-0.0603330281863-0.301210802711-0.03185223091080.1324007540111.023428982250.203056802034-0.1089390786060.5452790614490.04150042781480.736080217337-30.408690581749.6510389989-33.8010823067
1082.79920046836-2.73190037266-2.546083108268.182823473532.844408210844.18627257823-0.04408865838430.206857542428-0.157900877638-0.303950249568-0.05282936451040.139397054994-0.02882080986370.0506649671775-0.03055694386740.6694892835590.0912025988171-0.02443579377910.6130523463410.08866254748540.505564807528-28.410031647539.8200144848-44.1831329044
1091.451848267030.1837569962770.2522019250630.4289357563910.3311508213952.07983637172-0.0441451226759-0.1629168524880.009337041790430.116759776225-0.3589366508670.184754197188-0.3526123395010.1170216235820.1004239396970.6463684650740.161575599881-0.128690997880.379460541902-0.08189214257090.735351679227-18.089891723436.1086005529-1.69186077291
1104.005943329430.974986618981.604328714762.123413184090.4299417833493.30887688725-0.13195337409-0.102593913479-0.01116939390610.2281627453220.18473180317-0.1166779860560.0672437523399-0.09495735852010.007645596460760.274951661230.0673619994336-0.09596189895920.267453871099-0.1033313951850.590451602147-9.1218742675823.411238323326.5511977933
1111.09065290659-0.274373609830.17866892441.70196679221-0.4612607702361.43045022463-0.0146390687699-0.151050276980.298336078505-0.0780261810867-0.204507384506-0.07126657850760.0248595800217-0.2547289328250.1941460490160.2970232591560.0504910852569-0.08059749956920.312537339292-0.09674761518350.600543597341-11.564755250928.952545101516.7004224959
1121.43942733360.7432389357620.6979204835312.362117947741.518041007612.806075564390.07706064479490.1617339075420.289513617149-0.09446873065290.12911938121-0.158381746126-0.137699631665-0.0818501620322-0.06782902470780.3180258960460.0241341669135-0.07253785114420.220713623805-0.06042494482450.657352013488-3.114732705927.017951102615.6175084296
1131.869820888770.7689390844870.8690771401831.088043600131.023571629083.45414401702-0.226148568972-0.234398564301-0.0555743624989-0.275721070640.05532933095820.198798752946-0.2149078557740.1320521361110.02160241790970.3579925194770.107950111962-0.08492765529430.317185454101-0.1404961619630.576033326807-8.0330055198631.375415117325.6629857152
1140.189159764971-0.245117755240.4354965074861.15728394071-1.658207265534.102662117610.111944367815-0.143365891178-0.215310827441-0.297320874691-0.02519034812140.194782337903-0.0699811585031-0.364945794239-0.009256965016120.2970016967960.0848387793417-0.1409937597680.365356000849-0.2042415924450.737515060803-20.053381319228.641620910113.9425581163
1151.074164451650.4552656087731.742061064970.4300252840270.8236415408816.73154860133-0.198364818244-1.10240678301-0.234996060130.134502951196-0.152791158199-0.00746414107711-0.1362846456350.5495103771450.06550018212290.4820446439240.0468836801633-0.1132872478280.726130307556-0.1258146157340.816388140041-2.123761215229.81110060742.8229438118
1160.792781122240.3585775664920.7260289308180.944183895351-0.1107428328630.916288225963-0.0801912881503-0.4822608417930.611551670370.356968501099-0.08134403120720.705975050983-0.648687636831-0.4135486807480.04332455583921.055295132090.07643879731830.06069843632221.42841268234-0.05088053783041.17354384538-17.328689777341.051284662661.7659606137
1172.58760932684-0.281641569807-0.3855139379913.569646787261.122141364162.97194498691-0.1055743704050.246830756139-0.5356536523650.06110020859360.3204465310390.3800806299420.492181606153-0.560517451739-0.002481615059560.6897887424420.0175238825038-0.01212263910451.103801078560.02838784489560.945007958628-9.2293574151828.957735444151.9939997195
1184.370183705640.697541911022-0.9585093771.736653553940.2594553718872.02655666113-0.360301086909-0.42365495882-0.2196132987050.1917571226230.475356581843-0.01011562827110.13401546926-0.289814107997-0.128782449560.7459677411510.104711940894-0.05983741034920.8527830403190.05871747436710.724396838822-11.625943367834.055662281749.6570135097
1194.79847633565-1.50685264239-0.6246108736791.19490654008-0.0853657935180.8039609180240.0503608829751-0.18130533664-0.3006686150480.21964328059-0.0261359134271-0.173099382250.3688384514170.002725351476790.03237814518850.9053408010180.0270069292548-0.09300440835261.526057025550.3974427409341.49882943331-11.763386568528.199972519161.9272383927
1202.774360776190.113063464523-0.9375520278610.673951865885-0.2584219267032.2479865653-0.300822903534-0.2301977225330.444296827680.126382459521-0.131364310274-0.155366361233-0.544652290377-1.097267143680.07028815555410.5783329133030.269722020738-0.1031536609180.830676990962-0.2927363670460.821725286134-33.270914483242.762375813127.1553570806
1210.809586007144-0.145152768979-0.2344160187581.088274283480.7865066441751.25432911402-0.0544173789789-0.07603967523210.201294265067-0.238463757346-0.2331133832760.243069919271-0.344063953801-0.7001720769630.1123016560480.4295420992740.226811383474-0.149070912010.697796906761-0.2023043817090.729428862318-30.219474162538.03741695119.7785589888
1221.673302100230.07245071648140.4078671403580.687741938956-0.03451753331510.8974292657170.051492691539-0.631682144565-0.194299456144-0.0180963880653-0.4028510617480.192364019296-0.1909922928270.100429197610.2414285667240.5592899795460.00887908362635-0.05725484508830.96654478449-0.1938636133660.5405399731-15.965786489443.063669777950.8453863541
1232.475019875451.15226544751-0.1732304833062.71645718649-0.1226938629511.56568857842-0.228514874720.113369485507-0.00247689913080.1018065342070.2810257289590.326642968283-0.252106241359-0.176982011241-0.02265759466360.5618819852540.143024026549-0.07391519134820.689865053578-0.1434181050070.639297834171-21.806489729937.277681909544.5396143494
1241.028298033050.177200378026-0.04705925629120.7878472200820.07917857710940.209884731290.228265350573-0.04837157422310.06876480737920.381387382536-0.01610683767670.159766136039-0.2063327662460.220287829842-0.02178590516880.726891456670.0312764533498-0.08272219158460.817543198897-0.2307679597720.655832016855-12.29455142649.399576446850.2357656258
1253.386426433311.1267121881-0.01481571257862.149326005680.48510580821.479280050260.0658311366936-0.2662881778790.08830493602380.1666706293060.02715297558020.732201779631-0.541783554651-0.173442812775-0.1903982966350.6124531733890.207893009787-0.02620176883650.806316806499-0.2692845304450.845780681757-32.325270272648.464609922946.2478030434
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 28 )
2X-RAY DIFFRACTION2chain 'A' and (resid 29 through 56 )
3X-RAY DIFFRACTION3chain 'A' and (resid 57 through 84 )
4X-RAY DIFFRACTION4chain 'A' and (resid 85 through 118 )
5X-RAY DIFFRACTION5chain 'A' and (resid 119 through 137 )
6X-RAY DIFFRACTION6chain 'A' and (resid 138 through 162 )
7X-RAY DIFFRACTION7chain 'A' and (resid 163 through 174 )
8X-RAY DIFFRACTION8chain 'A' and (resid 175 through 197 )
9X-RAY DIFFRACTION9chain 'A' and (resid 198 through 227 )
10X-RAY DIFFRACTION10chain 'A' and (resid 228 through 274 )
11X-RAY DIFFRACTION11chain 'B' and (resid 1 through 11 )
12X-RAY DIFFRACTION12chain 'B' and (resid 12 through 22 )
13X-RAY DIFFRACTION13chain 'B' and (resid 23 through 41 )
14X-RAY DIFFRACTION14chain 'B' and (resid 42 through 51 )
15X-RAY DIFFRACTION15chain 'B' and (resid 52 through 90 )
16X-RAY DIFFRACTION16chain 'B' and (resid 91 through 99 )
17X-RAY DIFFRACTION17chain 'C' and (resid 1 through 9 )
18X-RAY DIFFRACTION18chain 'D' and (resid 2 through 18 )
19X-RAY DIFFRACTION19chain 'D' and (resid 19 through 63 )
20X-RAY DIFFRACTION20chain 'D' and (resid 64 through 91 )
21X-RAY DIFFRACTION21chain 'D' and (resid 92 through 107 )
22X-RAY DIFFRACTION22chain 'D' and (resid 108 through 119 )
23X-RAY DIFFRACTION23chain 'D' and (resid 120 through 132 )
24X-RAY DIFFRACTION24chain 'D' and (resid 133 through 153 )
25X-RAY DIFFRACTION25chain 'D' and (resid 154 through 168 )
26X-RAY DIFFRACTION26chain 'D' and (resid 169 through 181 )
27X-RAY DIFFRACTION27chain 'D' and (resid 182 through 191 )
28X-RAY DIFFRACTION28chain 'D' and (resid 192 through 214 )
29X-RAY DIFFRACTION29chain 'E' and (resid 3 through 135 )
30X-RAY DIFFRACTION30chain 'E' and (resid 136 through 256 )
31X-RAY DIFFRACTION31chain 'F' and (resid 2 through 28 )
32X-RAY DIFFRACTION32chain 'F' and (resid 29 through 56 )
33X-RAY DIFFRACTION33chain 'F' and (resid 57 through 84 )
34X-RAY DIFFRACTION34chain 'F' and (resid 85 through 137 )
35X-RAY DIFFRACTION35chain 'F' and (resid 138 through 162 )
36X-RAY DIFFRACTION36chain 'F' and (resid 163 through 174 )
37X-RAY DIFFRACTION37chain 'F' and (resid 175 through 219 )
38X-RAY DIFFRACTION38chain 'F' and (resid 220 through 274 )
39X-RAY DIFFRACTION39chain 'G' and (resid 1 through 11 )
40X-RAY DIFFRACTION40chain 'G' and (resid 12 through 21 )
41X-RAY DIFFRACTION41chain 'G' and (resid 22 through 41 )
42X-RAY DIFFRACTION42chain 'G' and (resid 42 through 51 )
43X-RAY DIFFRACTION43chain 'G' and (resid 52 through 90 )
44X-RAY DIFFRACTION44chain 'G' and (resid 91 through 99 )
45X-RAY DIFFRACTION45chain 'H' and (resid 1 through 9 )
46X-RAY DIFFRACTION46chain 'I' and (resid 2 through 18 )
47X-RAY DIFFRACTION47chain 'I' and (resid 19 through 63 )
48X-RAY DIFFRACTION48chain 'I' and (resid 64 through 91 )
49X-RAY DIFFRACTION49chain 'I' and (resid 92 through 107 )
50X-RAY DIFFRACTION50chain 'I' and (resid 108 through 119 )
51X-RAY DIFFRACTION51chain 'I' and (resid 120 through 132 )
52X-RAY DIFFRACTION52chain 'I' and (resid 133 through 145 )
53X-RAY DIFFRACTION53chain 'I' and (resid 146 through 163 )
54X-RAY DIFFRACTION54chain 'I' and (resid 164 through 191 )
55X-RAY DIFFRACTION55chain 'I' and (resid 192 through 214 )
56X-RAY DIFFRACTION56chain 'J' and (resid 3 through 135 )
57X-RAY DIFFRACTION57chain 'J' and (resid 136 through 173 )
58X-RAY DIFFRACTION58chain 'J' and (resid 174 through 201 )
59X-RAY DIFFRACTION59chain 'J' and (resid 202 through 256 )
60X-RAY DIFFRACTION60chain 'K' and (resid 2 through 28 )
61X-RAY DIFFRACTION61chain 'K' and (resid 29 through 56 )
62X-RAY DIFFRACTION62chain 'K' and (resid 57 through 84 )
63X-RAY DIFFRACTION63chain 'K' and (resid 85 through 137 )
64X-RAY DIFFRACTION64chain 'K' and (resid 138 through 162 )
65X-RAY DIFFRACTION65chain 'K' and (resid 163 through 174 )
66X-RAY DIFFRACTION66chain 'K' and (resid 175 through 219 )
67X-RAY DIFFRACTION67chain 'K' and (resid 220 through 262 )
68X-RAY DIFFRACTION68chain 'K' and (resid 263 through 275 )
69X-RAY DIFFRACTION69chain 'L' and (resid 1 through 11 )
70X-RAY DIFFRACTION70chain 'L' and (resid 12 through 19 )
71X-RAY DIFFRACTION71chain 'L' and (resid 20 through 30 )
72X-RAY DIFFRACTION72chain 'L' and (resid 31 through 41 )
73X-RAY DIFFRACTION73chain 'L' and (resid 42 through 51 )
74X-RAY DIFFRACTION74chain 'L' and (resid 52 through 71 )
75X-RAY DIFFRACTION75chain 'L' and (resid 72 through 90 )
76X-RAY DIFFRACTION76chain 'L' and (resid 91 through 99 )
77X-RAY DIFFRACTION77chain 'M' and (resid 1 through 9 )
78X-RAY DIFFRACTION78chain 'N' and (resid 2 through 18 )
79X-RAY DIFFRACTION79chain 'N' and (resid 19 through 63 )
80X-RAY DIFFRACTION80chain 'N' and (resid 64 through 91 )
81X-RAY DIFFRACTION81chain 'N' and (resid 92 through 119 )
82X-RAY DIFFRACTION82chain 'N' and (resid 120 through 132 )
83X-RAY DIFFRACTION83chain 'N' and (resid 133 through 153 )
84X-RAY DIFFRACTION84chain 'N' and (resid 154 through 168 )
85X-RAY DIFFRACTION85chain 'N' and (resid 169 through 191 )
86X-RAY DIFFRACTION86chain 'N' and (resid 192 through 214 )
87X-RAY DIFFRACTION87chain 'O' and (resid 3 through 135 )
88X-RAY DIFFRACTION88chain 'O' and (resid 136 through 256 )
89X-RAY DIFFRACTION89chain 'P' and (resid 2 through 28 )
90X-RAY DIFFRACTION90chain 'P' and (resid 29 through 56 )
91X-RAY DIFFRACTION91chain 'P' and (resid 57 through 84 )
92X-RAY DIFFRACTION92chain 'P' and (resid 85 through 118 )
93X-RAY DIFFRACTION93chain 'P' and (resid 119 through 137 )
94X-RAY DIFFRACTION94chain 'P' and (resid 138 through 162 )
95X-RAY DIFFRACTION95chain 'P' and (resid 163 through 174 )
96X-RAY DIFFRACTION96chain 'P' and (resid 175 through 257 )
97X-RAY DIFFRACTION97chain 'P' and (resid 258 through 275 )
98X-RAY DIFFRACTION98chain 'Q' and (resid 1 through 11 )
99X-RAY DIFFRACTION99chain 'Q' and (resid 12 through 19 )
100X-RAY DIFFRACTION100chain 'Q' and (resid 20 through 30 )
101X-RAY DIFFRACTION101chain 'Q' and (resid 31 through 41 )
102X-RAY DIFFRACTION102chain 'Q' and (resid 42 through 46 )
103X-RAY DIFFRACTION103chain 'Q' and (resid 47 through 51 )
104X-RAY DIFFRACTION104chain 'Q' and (resid 52 through 61 )
105X-RAY DIFFRACTION105chain 'Q' and (resid 62 through 71 )
106X-RAY DIFFRACTION106chain 'Q' and (resid 72 through 77 )
107X-RAY DIFFRACTION107chain 'Q' and (resid 78 through 90 )
108X-RAY DIFFRACTION108chain 'Q' and (resid 91 through 99 )
109X-RAY DIFFRACTION109chain 'R' and (resid 1 through 9 )
110X-RAY DIFFRACTION110chain 'S' and (resid 2 through 18 )
111X-RAY DIFFRACTION111chain 'S' and (resid 19 through 63 )
112X-RAY DIFFRACTION112chain 'S' and (resid 64 through 91 )
113X-RAY DIFFRACTION113chain 'S' and (resid 92 through 107 )
114X-RAY DIFFRACTION114chain 'S' and (resid 108 through 119 )
115X-RAY DIFFRACTION115chain 'S' and (resid 120 through 132 )
116X-RAY DIFFRACTION116chain 'S' and (resid 133 through 145 )
117X-RAY DIFFRACTION117chain 'S' and (resid 146 through 161 )
118X-RAY DIFFRACTION118chain 'S' and (resid 162 through 191 )
119X-RAY DIFFRACTION119chain 'S' and (resid 192 through 214 )
120X-RAY DIFFRACTION120chain 'T' and (resid 3 through 24 )
121X-RAY DIFFRACTION121chain 'T' and (resid 25 through 135 )
122X-RAY DIFFRACTION122chain 'T' and (resid 136 through 173 )
123X-RAY DIFFRACTION123chain 'T' and (resid 174 through 201 )
124X-RAY DIFFRACTION124chain 'T' and (resid 202 through 226 )
125X-RAY DIFFRACTION125chain 'T' and (resid 227 through 256 )

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more