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Yorodumi- PDB-8uxa: Glucose treated mitochondrial ribosome of saccharomyces cerevisia... -
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Open data
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Basic information
| Entry | Database: PDB / ID: 8uxa | |||||||||||||||||||||
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| Title | Glucose treated mitochondrial ribosome of saccharomyces cerevisiae class I | |||||||||||||||||||||
Components |
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Keywords | RIBOSOME / Dextrose treated mitochondrial ribosome | |||||||||||||||||||||
| Function / homology | Function and homology informationpositive regulation of mitochondrial DNA replication / mitochondrial translational initiation / mitochondrial respiratory chain complex IV assembly / Mitochondrial protein degradation / : / mitochondrial ribosome assembly / ribonuclease III activity / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial ribosome ...positive regulation of mitochondrial DNA replication / mitochondrial translational initiation / mitochondrial respiratory chain complex IV assembly / Mitochondrial protein degradation / : / mitochondrial ribosome assembly / ribonuclease III activity / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / RNA processing / cell redox homeostasis / mRNA processing / peroxisome / single-stranded DNA binding / double-stranded RNA binding / ribosome biogenesis / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / cellular response to oxidative stress / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / DNA recombination / cytosolic large ribosomal subunit / mitochondrial inner membrane / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / mRNA binding / regulation of DNA-templated transcription / GTP binding / mitochondrion / DNA binding / RNA binding / zinc ion binding / ATP binding / metal ion binding / nucleus / cytoplasm Similarity search - Function | |||||||||||||||||||||
| Biological species | ![]() | |||||||||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||||||||||||||
Authors | Yu, Z. / Zheng, F. / Zhou, C. | |||||||||||||||||||||
| Funding support | United States, 1items
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Citation | Journal: To Be PublishedTitle: Glucose treated mitochondrial ribosome of saccharomyces cerevisiae class I Authors: Yu, Z. / Zheng, F. / Zhou, C. #1: Journal: To Be PublishedTitle: The context-dependent plasticity and polymorphism of yeast mitochondrial ribosome Authors: Yu, Z. / Zheng, F. / Zhou, C. | |||||||||||||||||||||
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 8uxa.cif.gz | 4.7 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb8uxa.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 8uxa.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ux/8uxa ftp://data.pdbj.org/pub/pdb/validation_reports/ux/8uxa | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 42720MC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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Components
+Small ribosomal subunit ... , 25 types, 25 molecules AAABACAFAGAHAIAJAKALAMZhkmopqrsuvxyz
-37S ribosomal protein ... , 8 types, 8 molecules ADAEgjlntw
| #4: Protein | Mass: 36775.781 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRP1, YDR347W, D9651.1 / Production host: ![]() |
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| #5: Protein | Mass: 39045.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRP13, YGR084C / Production host: ![]() |
| #16: Protein | Mass: 39507.457 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRP51, YPL118W / Production host: ![]() |
| #19: Protein | Mass: 56450.949 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: NAM9, MNA6, YNL137C, N1211, N1840 / Production host: ![]() |
| #21: Protein | Mass: 15046.702 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRP17, YKL003C / Production host: ![]() |
| #23: Protein | Mass: 17496.602 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRPS8, YMR158W, YM8520.07 / Production host: ![]() |
| #29: Protein | Mass: 13563.061 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRP2, YPR166C, P9325.7 / Production host: ![]() |
| #32: Protein | Mass: 27683.986 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: MRPS17, YMR188C, YM8010.18C / Production host: ![]() |
-RNA chain , 2 types, 2 molecules ANA
| #14: RNA chain | Mass: 4084.518 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() |
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| #46: RNA chain | Mass: 1057803.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() |
-Protein , 1 types, 1 molecules i
| #18: Protein | Mass: 47170.996 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: VAR1, Q0140 / Production host: ![]() |
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+Large ribosomal subunit protein ... , 40 types, 40 molecules 0123456789BCDEFGHIJKLMNOPQRSTU...
-Details
| Has protein modification | N |
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-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: Mitochondrial ribosome Class I of saccharomyces cerevisiae cultured in YEP with Dextrose Type: RIBOSOME / Entity ID: #1-#75 / Source: NATURAL |
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| Source (natural) | Organism: ![]() |
| Buffer solution | pH: 7.5 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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| Microscopy | Model: FEI TECNAI ARCTICA |
| Electron gun | Electron source: LAB6 / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm |
| Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
| CTF correction | Type: NONE |
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| 3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 12907 / Symmetry type: POINT |
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