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- PDB-8uwz: The structure of Raamsizumab in complex with VEGF121 -

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Basic information

Entry
Database: PDB / ID: 8uwz
TitleThe structure of Raamsizumab in complex with VEGF121
Components
  • Isoform VEGF121 of Vascular endothelial growth factor A, long form
  • Raamsizumab heavy chain
  • Raamsizumab light chain S1C variant
KeywordsSIGNALING PROTEIN
Function / homology
Function and homology information


basophil chemotaxis / positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway / VEGF-A complex / Signaling by VEGF / cellular stress response to acid chemical / positive regulation of lymphangiogenesis / negative regulation of adherens junction organization / vascular endothelial growth factor receptor 1 binding / negative regulation of establishment of endothelial barrier / vascular endothelial growth factor receptor binding ...basophil chemotaxis / positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway / VEGF-A complex / Signaling by VEGF / cellular stress response to acid chemical / positive regulation of lymphangiogenesis / negative regulation of adherens junction organization / vascular endothelial growth factor receptor 1 binding / negative regulation of establishment of endothelial barrier / vascular endothelial growth factor receptor binding / VEGF ligand-receptor interactions / post-embryonic camera-type eye development / positive regulation of mast cell chemotaxis / lymph vessel morphogenesis / primitive erythrocyte differentiation / negative regulation of blood-brain barrier permeability / positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway / VEGF-activated neuropilin signaling pathway / bone trabecula formation / coronary vein morphogenesis / lung vasculature development / cardiac vascular smooth muscle cell development / lymphangiogenesis / endothelial cell chemotaxis / positive regulation of trophoblast cell migration / vascular endothelial growth factor receptor-2 signaling pathway / positive regulation of epithelial tube formation / VEGF binds to VEGFR leading to receptor dimerization / motor neuron migration / regulation of nitric oxide mediated signal transduction / positive regulation of protein localization to early endosome / eye photoreceptor cell development / positive regulation of axon extension involved in axon guidance / vascular wound healing / regulation of hematopoietic progenitor cell differentiation / positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / camera-type eye morphogenesis / positive regulation of branching involved in ureteric bud morphogenesis / neuropilin binding / coronary artery morphogenesis / induction of positive chemotaxis / transmembrane receptor protein tyrosine kinase activator activity / negative regulation of cell-cell adhesion mediated by cadherin / vascular endothelial growth factor receptor 2 binding / commissural neuron axon guidance / dopaminergic neuron differentiation / tube formation / positive regulation of vascular endothelial growth factor signaling pathway / positive regulation of vascular permeability / positive regulation of blood vessel branching / platelet-derived growth factor receptor binding / surfactant homeostasis / endothelial cell proliferation / extracellular matrix binding / cell migration involved in sprouting angiogenesis / epithelial cell maturation / positive regulation of leukocyte migration / positive regulation of positive chemotaxis / cardiac muscle cell development / sprouting angiogenesis / Regulation of gene expression by Hypoxia-inducible Factor / positive regulation of endothelial cell chemotaxis / vascular endothelial growth factor signaling pathway / artery morphogenesis / negative regulation of epithelial to mesenchymal transition / positive regulation of cell migration involved in sprouting angiogenesis / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of DNA biosynthetic process / retinal ganglion cell axon guidance / branching involved in blood vessel morphogenesis / positive regulation of neuroblast proliferation / negative regulation of fat cell differentiation / positive chemotaxis / positive regulation of sprouting angiogenesis / chemoattractant activity / mesoderm development / outflow tract morphogenesis / positive regulation of protein autophosphorylation / monocyte differentiation / macrophage differentiation / fibronectin binding / positive regulation of cell division / positive regulation of receptor internalization / mammary gland alveolus development / neuroblast proliferation / cellular response to vascular endothelial growth factor stimulus / positive regulation of focal adhesion assembly / positive regulation of blood vessel endothelial cell migration / vascular endothelial growth factor receptor signaling pathway / positive regulation of osteoblast differentiation / vasculogenesis / heart morphogenesis / cell maturation / ovarian follicle development / homeostasis of number of cells within a tissue / positive regulation of endothelial cell proliferation / lactation / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / epithelial cell differentiation / positive regulation of endothelial cell migration
Similarity search - Function
Vascular endothelial growth factor, heparin-binding domain / Vascular endothelial growth factor, heparin-binding domain superfamily / VEGF heparin-binding domain / : / PDGF/VEGF domain / Platelet-derived growth factor, conserved site / PDGF/VEGF domain / Platelet-derived growth factor (PDGF) family signature. / Platelet-derived growth factor (PDGF) family profile. / Platelet-derived and vascular endothelial growth factors (PDGF, VEGF) family / Cystine-knot cytokine
Similarity search - Domain/homology
Vascular endothelial growth factor A, long form
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.5 Å
AuthorsSinger, A.U. / Bruce, H. / Blazer, L. / Sicheri, F. / Adams, J.J. / Sidhu, S.S.
Funding support India, 1items
OrganizationGrant numberCountry
Other private India
CitationJournal: to be published
Title: Raamsizumab: A Potent Synthetic Human Anti-VEGF Neutralizing Fab for Treatment of Ocular Neovascularization Diseases
Authors: Fellouse, F.A. / Harit, R.K. / Arun, S. / Arora, R. / Wagner, N. / Antony, A. / Wong, S. / Yang, N. / Rajakrishnan, S. / Anthony, M. / Gokul, S. / Vinodh, S. / Bhunia, S. / Vinothini, T.M. / ...Authors: Fellouse, F.A. / Harit, R.K. / Arun, S. / Arora, R. / Wagner, N. / Antony, A. / Wong, S. / Yang, N. / Rajakrishnan, S. / Anthony, M. / Gokul, S. / Vinodh, S. / Bhunia, S. / Vinothini, T.M. / Choubey, S. / Rajendran, A. / Srivastava, A. / Seshagiri, S. / Gross, M.L. / Leung, D.W. / Amarasinghe, G.K. / Chaudhuri, A. / Sidhu, S.S. / Ramchand, C.N.
History
DepositionNov 8, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 13, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
C: Raamsizumab heavy chain
D: Raamsizumab light chain S1C variant
E: Isoform VEGF121 of Vascular endothelial growth factor A, long form
F: Isoform VEGF121 of Vascular endothelial growth factor A, long form
A: Raamsizumab heavy chain
B: Raamsizumab light chain S1C variant
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,1308
Polymers124,1196
Non-polymers1,0112
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)167.747, 167.747, 42.501
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number144
Space group name H-MP31
Space group name HallP31
Symmetry operation#1: x,y,z
#2: -y,x-y,z+1/3
#3: -x+y,-x,z+2/3
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 1 through 2 or (resid 3...
d_2ens_1(chain "C" and (resid 1 through 206 or (resid 207...
d_1ens_2(chain "B" and (resid 5 or (resid 6 and (name...
d_2ens_2(chain "D" and (resid 5 through 17 or (resid 18...
d_1ens_3(chain "E" and ((resid 11 and (name N or name...
d_2ens_3(chain "F" and (resid 11 through 55 or (resid 56...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1GLUGLUSERSERAE1 - 1421 - 134
d_12ens_1LYSLYSSERSERAE144 - 147136 - 139
d_13ens_1THRTHRALAALAAE150 - 151142 - 143
d_14ens_1LEULEUPROPROAE153 - 228145 - 220
d_21ens_1GLUGLUPROPROCA1 - 2281 - 220
d_11ens_2THRTHRSERSERBF5 - 265 - 26
d_12ens_2ALAALATHRTHRBF28 - 12928 - 109
d_13ens_2ALAALACYSCYSBF132 - 234112 - 212
d_21ens_2THRTHRCYSCYSDB5 - 2345 - 212
d_11ens_3HISHISLYSLYSEC11 - 10811 - 108
d_12ens_3NAGNAGNAGNAGGG1
d_21ens_3HISHISLYSLYSFD11 - 10811 - 108
d_22ens_3NAGNAGNAGNAGHH2

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(-0.954351434718, 0.298573908064, 0.00818293805804), (0.298450590061, 0.954328804419, -0.0135564873175), (-0.0118568268916, -0.0104954504293, -0.999874622728)11.5547041973, -2.37546391928, -45.1042859781
2given(-0.960848978196, 0.276761112001, 0.0131349908302), (0.276936042182, 0.960783901768, 0.0141676619053), (-0.00869882987619, 0.0172505358398, -0.999813357268)12.7457837286, -2.18057876966, -46.5452627584
3given(-0.964034585558, 0.265517542993, 0.0117367887998), (0.265579982151, 0.964080118588, 0.00409853925459), (-0.0102269706652, 0.00706818975165, -0.999922721896)12.9833088055, -1.74865459687, -45.9210972023

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Components

#1: Antibody Raamsizumab heavy chain


Mass: 24602.461 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pSCSTa / Cell line (production host): Expi293 / Production host: Homo sapiens (human)
#2: Antibody Raamsizumab light chain S1C variant


Mass: 23379.002 Da / Num. of mol.: 2 / Mutation: HAGLSSP replaced by QGTTS; Q165S, K167Y
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Details (production host): pSCSTa / Cell line (production host): Expi293 / Production host: Homo sapiens (human)
#3: Protein Isoform VEGF121 of Vascular endothelial growth factor A, long form


Mass: 14078.085 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P15692
#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.98 Å3/Da / Density % sol: 58.77 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.1 / Details: 0.2 M Sodium Phosphate mono basic, 30% PEG600 / Temp details: room temperature

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97915 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 7, 2023 / Details: mirrors
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97915 Å / Relative weight: 1
ReflectionResolution: 3.4→83.87 Å / Num. obs: 18391 / % possible obs: 99.8 % / Redundancy: 7.7 % / Biso Wilson estimate: 133.58 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.122 / Rpim(I) all: 0.046 / Rrim(I) all: 0.13 / Net I/σ(I): 9.2
Reflection shellResolution: 3.4→3.67 Å / Redundancy: 7.8 % / Rmerge(I) obs: 1.212 / Mean I/σ(I) obs: 1.6 / Num. unique obs: 3833 / CC1/2: 0.769 / Rpim(I) all: 0.458 / Rrim(I) all: 1.297 / % possible all: 99.9

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
MOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.5→83.87 Å / SU ML: 0.5771 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 39.9167
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2981 1696 10.09 %
Rwork0.237 15114 -
obs0.2433 16810 99.63 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 167.82 Å2
Refinement stepCycle: LAST / Resolution: 3.5→83.87 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7666 0 67 0 7733
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00487933
X-RAY DIFFRACTIONf_angle_d0.947710872
X-RAY DIFFRACTIONf_chiral_restr0.05521247
X-RAY DIFFRACTIONf_plane_restr0.01841394
X-RAY DIFFRACTIONf_dihedral_angle_d17.45592639
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2EAX-RAY DIFFRACTIONTorsion NCS0.984107314105
ens_2d_2FBX-RAY DIFFRACTIONTorsion NCS1.30684378744
ens_3d_2CEX-RAY DIFFRACTIONTorsion NCS1.39378384007
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.5-3.60.39351380.40871254X-RAY DIFFRACTION99.57
3.6-3.720.39021370.38911255X-RAY DIFFRACTION99.57
3.72-3.850.33531480.33961296X-RAY DIFFRACTION99.52
3.85-4.010.3521390.35521218X-RAY DIFFRACTION99.49
4.01-4.190.37211420.32631288X-RAY DIFFRACTION99.17
4.19-4.410.28081380.25221230X-RAY DIFFRACTION99.78
4.41-4.680.30011480.23971293X-RAY DIFFRACTION100
4.69-5.050.26861400.2341237X-RAY DIFFRACTION99.85
5.05-5.550.2971420.23161260X-RAY DIFFRACTION99.79
5.56-6.360.31311420.23831256X-RAY DIFFRACTION99.57
6.36-8.010.30871460.21681257X-RAY DIFFRACTION99.93
8.02-83.870.24621360.14891270X-RAY DIFFRACTION99.79
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.349132029880.9075089345922.458629244947.952878620314.743420254633.083874487460.426237173263-0.6190953497271.839997837980.993579216723-1.19661155021-1.41064454716-0.307488093994-0.2506187648850.4559176783961.49708032611-0.351652540319-0.05542539317740.9004585685520.0956089194032.1342628342328.75246.129-17.815
26.15924907012.142370177871.011797781472.17909301721-0.2509911370542.245629352150.111986602544-0.4973881683840.5505909891691.28181038759-0.610514402199-1.60571635965-0.5941229770830.442326133850.4227575240141.49391653922-0.326434878191-0.356273887940.9183127437120.2113139350762.1049945003535.01444.982-12.14
31.64235329477-0.2365578388290.2931933995317.672980762693.001100850756.06150607415-0.09577851148850.172772935317-0.02254579066280.866214064063-0.745270358487-1.02189539721-0.1091086662220.6068843596860.8610668126061.53993635046-0.242302403233-0.6290907939821.205442280880.2367778202672.7765561132754.80359.448-19.456
43.01500378837-2.414727096572.834099999635.320201997380.5063127525245.37871576315-0.312627172035-1.114332240812.05734677182-0.126908439622-0.168742340241-2.003494199920.3670414332330.5481442477710.8500422719632.19045105775-0.060337987803-0.4791917500651.106268025970.3735363880232.9728352634347.98725.707-9.352
55.07928899430.444958378167-1.761569329973.154079822613.536195075014.57199283329-0.908294730581.49537434063-1.134799856191.41646572071.28677931108-0.8744673034680.8678366930220.8919028687230.199388804441.56991561498-0.0572359599296-0.5611427031190.8554386379680.06823848582313.3940954287643.69129.194-19.968
63.754711149391.55427180569-1.345226625554.453847419941.412619674062.0733168671-0.111368532584-0.353928298209-2.867701788461.44752536942-0.456924435505-1.61662782620.7284401823610.7197774529110.2649618106742.13082141612-0.0831555996633-0.7732725273771.291642029250.4877893792933.3991285346244.32126.995-11.515
78.18541922763-3.96926826022-2.828865875572.15070312140.3613504566490.5303504623550.2985247958811.050303926951.66587778842.48992915735-0.477219354858-2.450624361310.3426420481391.280362243090.5113463466621.725147995810.0467414836804-0.7676588148912.259743193830.1644758072753.8037448123864.65556.358-12.664
81.574009351492.814768253211.055100298124.216249577281.528689711778.950123044410.5043756775571.238621334091.065660549461.94329287679-1.05090310554-1.881542469970.125298631483.010593410320.1069906666651.719128102440.20092064746-0.95454761981.98228480007-0.07667326734663.7445864911267.68254.414-6.736
92.851253612462.512260651172.377990040782.392742138721.564976154129.06880262171-1.064722977510.4194980352511.00792689111.247751957220.271616057317-0.480886128033-1.272622638721.750616488690.1772211977391.87869442358-0.360403556567-0.7924092581891.623581471480.3819069945783.3909049932465.42757.036-6.272
104.09926626802-5.40005860576-4.279244060416.228427409273.786229861264.486768255612.81084789615-4.1035299033-2.703837567941.333434621490.355818973004-4.48873715703-1.437790207790.404725737213-1.768875431561.14962101734-0.988348969341-1.920796817692.805835559111.212473168545.792093376375.40258.081-7.276
112.011364062537.212460853579.389341737923.368091455634.252963377845.6231859650.883384969894-2.723263385580.252462600302-0.587609470367-0.0873559988487-3.235407532431.12564154522-1.1060181639-0.3212733426222.055340217050.1230602722430.4534985747932.601714304640.1514537047840.8134686559111.44933.79-5.924
125.86941168959-1.81579780803-0.7021070114138.542944514417.474028949798.076773611830.940991701221-1.30241820434-0.395271780343-0.596054788827-2.020011250231.896661185580.228798211921-0.731862047791.243896737781.21643537556-0.2754630608610.2515843528490.944873899677-0.1052970878811.09400402321-4.44131.594-18.453
133.97042950462-1.18304210423-1.185454967152.811579947260.6838420800391.290880861831.42471478953-0.295925002814-3.25378801650.669382991052-1.158878376511.50496332881-0.205083574492-0.158374986491-0.4852469833581.55934842045-0.1228783246240.3020636747780.881237588424-0.02595249299721.550928293659.80220.703-28.357
149.58901496479-0.339267787555.461768211012.078789144721.846588554242.07969357892-0.140761423141.090704049751.24468482662-2.127632801850.745527673785-1.61601164366-1.91607605883-1.78026808212-0.6439418118721.687107469670.378823340438-0.1609586684731.41690142070.1893525434661.5228054180424.1319.981-36.716
154.465512414491.473369933431.497943073897.40060974441.4618977437.764167825791.82804294666-1.1091066852-0.108463618162-0.839199582256-0.872965880977-0.960335505263-0.0705085706953-0.766904827591-1.075797486360.862821625024-0.06835818281840.2592722514221.10840420009-0.008639736847091.2432718318119.96219.678-29.759
168.24824382776-8.70027341732-9.128380932369.319936555338.385918887592.14358712205-1.88194469968-0.371614271771-3.358304652491.4851822791-0.6599323080061.597384458290.629901927086-0.2541001125352.62818008551.69141166989-0.5211082334170.3706159246491.422197077680.07591847691281.20843772401-5.4521.15-26.077
171.97673592704-0.470759888597-1.545474272815.7884320401-4.36817773764.286743725570.3491175463191.29087519630.4597814269380.170350086676-0.607967951362-0.05677963647671.3029746836-0.2847931424120.3090789232631.21299857442-0.1813783482380.2662044951790.85735831479-0.3561322058391.2704575820613.50721.743-35.576
182.052157810943.91305439697-1.181019351531.993642719797.624430961641.991152121332.957750365460.856665785445-1.92095321998-3.534413181820.3637384585471.25385487467-4.159372707695.29265291519-3.581242402732.48194199371-0.1847947143130.1979751329751.93113552356-0.8042895133482.8824440583236.71813.333-34.381
193.29772196545-0.8286106169-0.417167419981.68330242732.156403726915.135530698931.897989298930.6782059455053.488399563071.36283020156-0.5359115229931.507886657331.126545315020.857085809034-0.7129889316311.474506385110.2859618069920.4614655679671.002433278870.1543257795191.8987572808118.0321.687-37.339
204.29737699837-1.791800084520.9978016194491.595217411122.110869132528.869769761710.626758777540.6244865111860.359531410524-0.184869450635-1.699432088890.690813910204-0.275054788862-1.449175231080.4121890618920.9757962165340.01497801956770.461871817971.29879466607-0.4326416240521.68017910176-3.58222.228-34.668
211.61551273511-2.618547217035.411923631764.57426970228-9.528348177252.057532504641.544712944871.49703892904-0.351186630565-2.575802191241.363129518823.33581580788-0.776424986635-3.45938364112-2.313957109182.24915492460.2008369947350.2238138872941.481569043930.3742686363731.823758951720.55430.636-39.742
227.2979863762-0.0717517274231.773520702477.38390590758-7.571549136498.5619767501-1.232435748540.5412534081240.8275327969252.25233994088-0.461344744021-0.335819373977-0.441879486934-0.7461600135561.701081635991.211781529590.07742883860650.3467361555920.848919477980.08320323601071.3916421129725.22627.113-27.273
238.64481187644.4573858101-3.235132057643.01617294815-0.9775515736011.754299810020.991213874514-0.228847392307-2.567611031161.16598102706-0.812025264472-1.363104326120.328325777606-0.808004497573-0.1566646004242.05397953949-0.468156188185-0.04689015974961.17571947349-0.08894989390510.8133881434219.4721.624-17.787
246.7172956106-6.621335942482.409163058929.535510328691.107688688875.426066470881.24376242592-1.25724474144-2.6884928318-0.308899927004-0.653607741622.71017583281.38621163904-0.548740408903-0.3191527152641.23040647097-0.299786155306-0.005008113458651.2252428781-0.1567958159041.80067612097-8.11616.996-12.08
254.845571974550.3893092960440.9249284386441.734075258551.166309128754.75640393286-0.21887404419-0.147425528976-0.9105678044531.179170246970.108005972822-0.0133095915196-0.1089129998630.03077503242930.2457880327931.86250143133-0.28180164287-0.01336633394210.894030876149-0.02132247101081.107010857738.82421.339-13.388
264.92302365373.60287372895-2.386086953877.24735706376-2.419355940222.386840017370.13472301363-1.578887427661.792946605230.347868523788-0.4070249010961.517410143-0.839126695110.6073817391750.1934427245651.4129292337-0.1938828817970.3623154049351.10768524149-0.2522662824451.02643175057-7.03722.358-10.373
279.33554178349-5.079348173731.176690778268.284716391571.826204922832.67212756979-1.587053734121.71271042815-0.2725102641661.919128393421.221549307310.5596755546050.62005658693-0.7067952153190.5555113826531.73246503204-0.494742306759-0.2313772774721.364778137160.2811592525461.4366991354522.30317.549-10.717
283.70365818071-4.343950752623.472988510647.70500546405-7.458623760252.50337280772-1.084851337830.6628274584020.3151506057370.457609395473-1.28474325649-2.384062702060.518459210939-0.2005363552282.752757814281.5811568933-0.312126429510.265211372190.917697430338-0.01014509086271.55444953964-4.66157.263-31.852
292.43540892894-8.075196487952.367029794025.94225389797-1.101224893193.588212692970.0688257716124-0.446706092728-0.0836202384813-0.05121219971390.157620052002-1.051587077390.357402678942-0.11738537667-0.07185202759461.25056317846-0.2965372744010.2106571717530.9828705238770.01911507682951.29076780531-0.46445.651-24.54
3010.6325879945-0.7924548912251.842608579216.5077743422-4.897327270594.210872217750.4759927264471.57409083606-0.641609518820.116301233109-0.4601911607750.378103444941-0.06446789094780.066721881135-0.01583649209861.10584147592-0.08599168848670.2170483992560.964054124237-0.2223544941471.11556133993-4.61744.529-37.624
315.92961424219-1.51944439330.3521571994952.53612407513-4.516023781662.216893038190.8825837595410.1151031049971.333930965121.1824957799-0.62592118151-1.53713739021-1.896879477362.56784289141-0.09284811815621.34234182267-0.2314688164870.1588321186241.1436460838-0.1810707226481.858733426942.91549.697-30.394
324.7989909929-2.09976283839-0.9340751804166.83565969124-5.03948568225.35130260148-0.1212853768820.287086671497-0.7963748966751.002654322820.5405735310580.549969150688-0.523414088045-0.31289732622-0.4323971062491.2050360826-0.09605562255640.1787637437940.640690429617-0.2507541949341.30244987406-7.84145.962-34.195
331.89463518161-1.41777690212.477999329221.50452989625-0.7682169347412.05595861299-0.206910174811-0.3706779924241.141366116190.5088315538690.2977109811120.989340870266-1.65819728587-0.0599064417961-0.2413986675391.59799215410.1940776699980.07648611743980.865491696154-0.2309594028341.88853058398-13.2462.818-32.734
344.98929064814-3.957372270852.43737162377.364321602650.6433292834076.2126237891-0.8405292155640.2206714436210.3195271856150.7986764852020.3997840231690.579916171619-1.15013460497-1.399157124610.7844578381481.848409090610.08372617742650.4376360104971.08981524769-0.2177054423022.58577269342-32.22375.457-27.238
353.09380981142-3.857923536810.6662743375825.689482543690.5921306560314.380421559160.696270165279-1.41356310272-0.344867429551.9603524286-0.08215939215050.104878521297-2.529147335110.151763804376-0.6297164180592.10862611075-0.1393689476850.4720783897161.098721647410.171062532341.86687441433-22.10167.454-24.465
363.151845301-1.63393613946-0.2884813615962.71331423122-0.5700564010431.788464086550.09071816627831.767367992390.710123720215-0.182044252917-0.198972007513-0.892820117101-0.304755005816-1.179191413040.6220397437991.784793553320.1427139867190.5075368717141.105438397810.1619558073391.61745041435-25.08869.772-33.839
372.53856071597-0.03057818043216.10736753242.820663650250.6372579679422.99378925934-1.78778060337-4.20406926951-0.4310349332342.487441303590.04493536357910.347708046223-2.293540385720.9232042855611.5948284553.31647396955-0.05255416370150.08085407704011.801535291940.07361824923951.45009129273-26.46874.906-18.627
382.23925871717-4.61267917281-8.380892847212.460152847851.62749739488.25244810796-0.551351785607-2.733297044533.64089188726-0.9327653228921.32828344042-0.623313504221-2.330182673592.04173050178-0.5450607589811.59421074756-0.219265410497-0.0662498349451.68815242219-0.4842650327752.74012773994-18.52476.842-24.359
396.892872341515.3948757874-2.209174868848.56964160351-6.794603203466.873464253111.70918325569-1.107445379784.25632043594-0.0761365161019-0.03846274434252.83754776401-1.61496961383-0.173144879436-1.255900263051.07704252549-0.01711837647350.08229280447191.4772195582-0.2274983941332.84461640281-33.62341.85-37.083
405.540387539640.3062761289123.846437025228.710905083080.7946268784455.107815834950.5030930643340.85881207893-0.196290361465-0.846472485289-1.216867466581.79671222639-0.8509112202570.1076242190680.7789809643841.150041411570.01035918306570.3791570328790.895136794898-0.08466066515522.33599736704-20.35637.533-35.024
415.37799541022-5.002322836251.367913728275.408166886592.559400314933.216363379290.07678006356950.269840419926-1.134482933881.21112588116-0.075343157922.52696590280.341450779881-0.688609015641-0.1249066669191.50797642322-0.2665133282990.5003904683810.984076168959-0.09898983010852.77067485384-26.28235.445-28.543
427.6507990749-2.65033300836-3.877271120032.613228414821.700141601662.938740971721.596402528220.5775587622621.01484000752-0.865390090566-0.659085762446-0.275478970273-0.567890568084-0.523866166139-1.126419101541.13942465693-0.09706448083830.2611451203670.952655927455-0.118951003661.88897409026-15.27839.113-37.977
431.94334235995-0.309348084625-1.194714193171.613924354620.8157313363772.247954485972.89786039760.4686692287771.61940290971-2.05841032399-1.79670268612-0.369593262581-0.799241030023-4.72755796855-0.9574468831720.9894637441081.875815899651.18771787462-0.229714253654-0.2701019347252.94144461634-39.21952.239-33.162
442.688480881591.89368296366-1.404883963914.455548164982.528476179163.697925700542.1554427964-1.093376551360.9180003203593.53847938307-1.07381922561.59706076583-1.3821208630.365216464557-0.7207246304262.499001977070.1286691810710.4468046128151.176573053310.268255587191.61025279118-31.4581.071-32.484
456.694374637133.47977858418-3.70140115555.597212330791.442313850548.019216588150.1068096550460.2867510465261.331882507910.702216635029-0.4609011742712.19876451323-0.54906411538-0.2463006961540.2443487610972.111146273440.2154803592860.4509815029910.9956434283220.3427436377992.06668193046-35.24871.175-33.827
467.02787310988-4.393449729111.77420301756.370055825052.906543857175.294072255370.3901801031573.25303606599-1.581097107550.001336623435930.005994415586343.17040902398-0.360708008642-1.50528561279-0.7897895512561.362707396870.1016793846670.3895480042171.66235453479-0.06261336859522.16798467411-40.24967.858-41.455
477.66480169419-2.69490383784.581849420088.6035642155-9.441804038182.816684151-1.798581700140.0704284071864-1.995131941114.549822841871.922240721232.57978947342-3.34713719933-1.19937736798-0.007747276963382.811672026370.3982873383670.3808648190791.075318333440.08492381093452.15945081159-35.466.186-37.316
487.87245964682-3.26102053013-0.2216145233424.995167309865.260298340047.477868667890.001400421264341.055048934122.13888269884-0.2666577254240.366523998992.11058671363-1.223650634110.412320749839-0.09370878702381.66053554859-0.03476374443480.09187128869541.244716388410.3187966043571.62406855801-32.66177.047-39.095
498.31023391288-3.460503617840.2408344501213.739492523234.072012745847.8305561387-0.4035735883720.9849191702530.8020375593771.313952773470.1024465562761.39908136421-1.44083599685-0.4561441720330.645213838511.729503012270.07702001458390.3586273705461.2773563390.4238820176622.0845372621-41.77976.25-40.688
508.43207230958-4.17402763232-0.8209474073654.511007836742.526514263278.52617211496-1.365379154810.9588941010411.738898375510.8074914315490.4943074003321.63589372513-0.4669147174290.320357137780.7693434600191.945956190340.3705331985760.2293115218321.466195900960.259659505812.13503958285-43.9977.044-35.931
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN C AND RESID 1:37 )C1 - 37
2X-RAY DIFFRACTION2( CHAIN C AND RESID 38:153 )C38 - 153
3X-RAY DIFFRACTION3( CHAIN C AND RESID 154:228 )C154 - 228
4X-RAY DIFFRACTION4( CHAIN D AND RESID 5:44 )D5 - 44
5X-RAY DIFFRACTION5( CHAIN D AND RESID 45:75 )D45 - 75
6X-RAY DIFFRACTION6( CHAIN D AND RESID 76:122 )D76 - 122
7X-RAY DIFFRACTION7( CHAIN D AND RESID 123:148 )D123 - 148
8X-RAY DIFFRACTION8( CHAIN D AND RESID 149:170 )D149 - 170
9X-RAY DIFFRACTION9( CHAIN D AND RESID 171:210 )D171 - 210
10X-RAY DIFFRACTION10( CHAIN D AND RESID 211:234 )D211 - 234
11X-RAY DIFFRACTION11( CHAIN E AND RESID 11:16 )E11 - 16
12X-RAY DIFFRACTION12( CHAIN E AND RESID 17:23 )E17 - 23
13X-RAY DIFFRACTION13( CHAIN E AND RESID 24:38 )E24 - 38
14X-RAY DIFFRACTION14( CHAIN E AND RESID 39:45 )E39 - 45
15X-RAY DIFFRACTION15( CHAIN E AND RESID 46:56 )E46 - 56
16X-RAY DIFFRACTION16( CHAIN E AND RESID 57:65 )E57 - 65
17X-RAY DIFFRACTION17( CHAIN E AND RESID 66:83 )E66 - 83
18X-RAY DIFFRACTION18( CHAIN E AND RESID 84:90 )E84 - 90
19X-RAY DIFFRACTION19( CHAIN E AND RESID 91:99 )E91 - 99
20X-RAY DIFFRACTION20( CHAIN E AND RESID 100:108 )E100 - 108
21X-RAY DIFFRACTION21( CHAIN F AND RESID 11:16 )F11 - 16
22X-RAY DIFFRACTION22( CHAIN F AND RESID 17:23 )F17 - 23
23X-RAY DIFFRACTION23( CHAIN F AND RESID 24:38 )F24 - 38
24X-RAY DIFFRACTION24( CHAIN F AND RESID 39:50 )F39 - 50
25X-RAY DIFFRACTION25( CHAIN F AND RESID 51:84 )F51 - 84
26X-RAY DIFFRACTION26( CHAIN F AND RESID 85:99 )F85 - 99
27X-RAY DIFFRACTION27( CHAIN F AND RESID 100:109 )F100 - 109
28X-RAY DIFFRACTION28( CHAIN A AND RESID 1:18 )A1 - 18
29X-RAY DIFFRACTION29( CHAIN A AND RESID 19:38 )A19 - 38
30X-RAY DIFFRACTION30( CHAIN A AND RESID 39:75 )A39 - 75
31X-RAY DIFFRACTION31( CHAIN A AND RESID 76:91 )A76 - 91
32X-RAY DIFFRACTION32( CHAIN A AND RESID 92:114 )A92 - 114
33X-RAY DIFFRACTION33( CHAIN A AND RESID 115:139 )A115 - 139
34X-RAY DIFFRACTION34( CHAIN A AND RESID 140:160 )A140 - 160
35X-RAY DIFFRACTION35( CHAIN A AND RESID 161:180 )A161 - 180
36X-RAY DIFFRACTION36( CHAIN A AND RESID 181:200 )A181 - 200
37X-RAY DIFFRACTION37( CHAIN A AND RESID 201:215 )A201 - 215
38X-RAY DIFFRACTION38( CHAIN A AND RESID 216:229 )A216 - 229
39X-RAY DIFFRACTION39( CHAIN B AND RESID 5:18 )B5 - 18
40X-RAY DIFFRACTION40( CHAIN B AND RESID 19:54 )B19 - 54
41X-RAY DIFFRACTION41( CHAIN B AND RESID 55:99 )B55 - 99
42X-RAY DIFFRACTION42( CHAIN B AND RESID 100:121 )B100 - 121
43X-RAY DIFFRACTION43( CHAIN B AND RESID 122:133 )B122 - 133
44X-RAY DIFFRACTION44( CHAIN B AND RESID 134:148 )B134 - 148
45X-RAY DIFFRACTION45( CHAIN B AND RESID 149:159 )B149 - 159
46X-RAY DIFFRACTION46( CHAIN B AND RESID 160:170 )B160 - 170
47X-RAY DIFFRACTION47( CHAIN B AND RESID 171:192 )B171 - 192
48X-RAY DIFFRACTION48( CHAIN B AND RESID 193:207 )B193 - 207
49X-RAY DIFFRACTION49( CHAIN B AND RESID 208:218 )B208 - 218
50X-RAY DIFFRACTION50( CHAIN B AND RESID 219:234 )B219 - 234

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