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Open data
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Basic information
Entry | Database: PDB / ID: 8uct | |||||||||
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Title | Crystal structure of TcPINK1 in complex with PRT | |||||||||
![]() | Serine/threonine-protein kinase Pink1, mitochondrial | |||||||||
![]() | CELL CYCLE / Mitophagy Autophagy Kinase | |||||||||
Function / homology | ![]() positive regulation of free ubiquitin chain polymerization / positive regulation of mitochondrial fission / positive regulation of protein ubiquitination / autophagy / peptidyl-serine phosphorylation / regulation of apoptotic process / mitochondrial outer membrane / protein autophosphorylation / mitochondrial inner membrane / non-specific serine/threonine protein kinase ...positive regulation of free ubiquitin chain polymerization / positive regulation of mitochondrial fission / positive regulation of protein ubiquitination / autophagy / peptidyl-serine phosphorylation / regulation of apoptotic process / mitochondrial outer membrane / protein autophosphorylation / mitochondrial inner membrane / non-specific serine/threonine protein kinase / protein serine/threonine kinase activity / ubiquitin protein ligase binding / mitochondrion / ATP binding / metal ion binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Veyron, S. / Rasool, S. / Trempe, J.F. | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Characterization of a new family of PINK1 inhibitors Authors: Shomali, T. / Rasool, S. / Croteau, N. / Veyron, S. / Truong, L. / Trempe, J.F. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 256.3 KB | Display | ![]() |
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PDB format | ![]() | 185.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 772.3 KB | Display | ![]() |
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Full document | ![]() | 778.9 KB | Display | |
Data in XML | ![]() | 16.7 KB | Display | |
Data in CIF | ![]() | 22.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51032.293 Da / Num. of mol.: 1 / Mutation: W131A, W142A, Y225A, Y378A, F401A, F437A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: D6WMX4 | ||||||
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#2: Chemical | ChemComp-DTT / | ||||||
#3: Chemical | #4: Chemical | ChemComp-3YT / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.71 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 100mM HEPES, PEG 4000, 150mM ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Nov 27, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 2.93→90.93 Å / Num. obs: 11014 / % possible obs: 100 % / Redundancy: 17.8 % / Biso Wilson estimate: 75.42 Å2 / CC1/2: 0.995 / Rpim(I) all: 0.145 / Net I/σ(I): 6.1 |
Reflection shell | Resolution: 2.93→2.98 Å / Redundancy: 16.9 % / Mean I/σ(I) obs: 0.5 / Num. unique obs: 521 / CC1/2: 0.551 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 76.82 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.93→90.93 Å
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Refine LS restraints |
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LS refinement shell |
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