Mass: 26525.217 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
Mass: 18.015 Da / Num. of mol.: 82 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.03 Å3/Da / Density % sol: 39.44 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1:1 ratio of well solution (25% PEG + 0.2 M MgCl2) and protein solution (LEA H62X at 15 mg/mL in 10 mM HEPES pH 7.5 + 75 mM NaCl).
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N