- PDB-8u2o: Crystal Structure of Cdk-related protein kinase 6 (PK6) from Plas... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 8u2o
Title
Crystal Structure of Cdk-related protein kinase 6 (PK6) from Plasmodium falciparum in complex with inhibitor TCMDC-123995
Components
Protein kinase 6
Keywords
TRANSFERASE / SSGCID / STRUCTURAL GENOMICS / SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE / Protein kinase 6 / inhibitor TCMDC-123995
Function / homology
Function and homology information
mediator complex / cyclin-dependent kinase / cyclin-dependent protein serine/threonine kinase activity / protein phosphorylation / ATP binding / nucleus / cytoplasm Similarity search - Function
Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 132 / Source method: isolated from a natural source / Formula: H2O
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Details
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.33 Å3/Da / Density % sol: 47.29 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: Index G5: 25% (w/v) PEG 3350, 0.1 M Tris pH 8.5, 0.2 M Lithium sulfate monohydrate. PlfaA.01252.a.A13.PW39218 at 15.9 mg/mL. His tag cleaved with 3C protease. 2mM TCMDC-123995 added prior to ...Details: Index G5: 25% (w/v) PEG 3350, 0.1 M Tris pH 8.5, 0.2 M Lithium sulfate monohydrate. PlfaA.01252.a.A13.PW39218 at 15.9 mg/mL. His tag cleaved with 3C protease. 2mM TCMDC-123995 added prior to crystallization. plate EWC2 well E12. Puck: PSL-1008, Cryo: 80% crystallant + 20% PEG200.
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
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