National Institutes of Health/National Cancer Institute (NIH/NCI)
1R01GM141659
United States
Citation
Journal: Sci Adv / Year: 2024 Title: Structural mechanisms of Tad pilus assembly and its interaction with an RNA virus. Authors: Yuhang Wang / Matthew Theodore / Zhongliang Xing / Utkarsh Narsaria / Zihao Yu / Lanying Zeng / Junjie Zhang / Abstract: Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV ... Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV pilins, Tad pilins lack the typical globular β sheet domain responsible for pilus assembly and phage binding. The mechanisms of Tad pilus assembly and its interaction with phage ΦCb5 have been elusive. Using cryo-electron microscopy, we unveiled the Tad pilus assembly mechanism, featuring a unique network of hydrogen bonds at its core. We then identified the Tad pilus binding to the ΦCb5 maturation protein (Mat) through its β region. Notably, the amino terminus of ΦCb5 Mat is exposed outside the capsid and phage/pilus interface, enabling the attachment of fluorescent and affinity tags. These engineered ΦCb5 virions can be efficiently assembled and purified in , maintaining infectivity against , which presents promising applications, including RNA delivery and phage display.
A: Flp family type IVb pilin J: Flp family type IVb pilin K: Flp family type IVb pilin L: Flp family type IVb pilin M: Flp family type IVb pilin N: Flp family type IVb pilin O: Flp family type IVb pilin P: Flp family type IVb pilin Q: Flp family type IVb pilin R: Flp family type IVb pilin S: Flp family type IVb pilin T: Flp family type IVb pilin U: Flp family type IVb pilin V: Flp family type IVb pilin W: Flp family type IVb pilin X: Flp family type IVb pilin Y: Flp family type IVb pilin Z: Flp family type IVb pilin a: Flp family type IVb pilin b: Flp family type IVb pilin c: Flp family type IVb pilin d: Flp family type IVb pilin e: Flp family type IVb pilin f: Flp family type IVb pilin g: Flp family type IVb pilin h: Flp family type IVb pilin i: Flp family type IVb pilin j: Flp family type IVb pilin k: Flp family type IVb pilin l: Flp family type IVb pilin m: Flp family type IVb pilin n: Flp family type IVb pilin o: Flp family type IVb pilin p: Flp family type IVb pilin q: Flp family type IVb pilin r: Flp family type IVb pilin s: Flp family type IVb pilin
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