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Yorodumi- PDB-8tp8: Structure of the C. crescentus WYL-activator, DriD, bound to ssDN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8tp8 | ||||||
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Title | Structure of the C. crescentus WYL-activator, DriD, bound to ssDNA and cognate DNA | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / WYL motif / DriD / C. crescentus / DNA damage repair / ssDNA / transcription activation / TRANSCRIPTION / TRANSCRIPTION-DNA complex | ||||||
Function / homology | Protein PafC / WYL domain / WYL domain / DNA / DNA (> 10) / DeoR-family transcriptional regulator Function and homology information | ||||||
Biological species | Caulobacter vibrioides NA1000 (bacteria) Caulobacter vibrioides (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.74 Å | ||||||
Authors | Schumacher, M.A. | ||||||
Funding support | United States, 1items
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Citation | Journal: Nucleic Acids Res. / Year: 2024 Title: Structure of the WYL-domain containing transcription activator, DriD, in complex with ssDNA effector and DNA target site. Authors: Schumacher, M.A. / Cannistraci, E. / Salinas, R. / Lloyd, D. / Messner, E. / Gozzi, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8tp8.cif.gz | 615.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8tp8.ent.gz | 502.8 KB | Display | PDB format |
PDBx/mmJSON format | 8tp8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8tp8_validation.pdf.gz | 526.8 KB | Display | wwPDB validaton report |
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Full document | 8tp8_full_validation.pdf.gz | 561.6 KB | Display | |
Data in XML | 8tp8_validation.xml.gz | 52 KB | Display | |
Data in CIF | 8tp8_validation.cif.gz | 72 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tp/8tp8 ftp://data.pdbj.org/pub/pdb/validation_reports/tp/8tp8 | HTTPS FTP |
-Related structure data
Related structure data | 8tpkC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 38037.203 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Caulobacter vibrioides NA1000 (bacteria) Gene: CCNA_01151 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0H3C5Q6 |
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-DNA chain , 3 types, 8 molecules UFRTLYJK
#2: DNA chain | Mass: 6437.183 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Caulobacter vibrioides (bacteria) #3: DNA chain | Mass: 6446.197 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Caulobacter vibrioides (bacteria) #4: DNA chain | Mass: 877.623 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Caulobacter vibrioides (bacteria) |
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-Non-polymers , 2 types, 114 molecules
#5: Chemical | ChemComp-SO4 / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62.17 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.1 M Tris, 1.0 M Citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 3, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.74→69 Å / Num. obs: 56088 / % possible obs: 96.1 % / Redundancy: 3.3 % / CC1/2: 0.99 / Rmerge(I) obs: 0.092 / Rpim(I) all: 0.06 / Net I/σ(I): 7.6 |
Reflection shell | Resolution: 2.74→2.89 Å / Rmerge(I) obs: 0.657 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 3276 / CC1/2: 0.635 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.74→68.97 Å / SU ML: 0.45 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 28.59 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.74→68.97 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 29.1364 Å / Origin y: -16.2606 Å / Origin z: 45.204 Å
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Refinement TLS group | Selection details: all |