PDB-8tlf: CDCA7 (Mouse) Binds Non-B-form DNA oligo 36-mer (sg C2-Form 2)

ID or keywords:

Entry

Database: PDB / ID: 8tlf

Title

CDCA7 (Mouse) Binds Non-B-form DNA oligo 36-mer (sg C2-Form 2)

Components

Cell division cycle-associated protein 7
ssDNA

Keywords

DNA BINDING PROTEIN/DNA / PROTEIN-DNA COMPLEX / DNA BINDING PROTEIN / zinc fingers / chromatin architecture / DNA BINDING PROTEIN-DNA complex

Function / homology

Function and homology information

regulation of cell population proliferation / regulation of DNA-templated transcription / apoptotic process / nucleoplasm / nucleus / cytosol
Similarity search - Function

Biological species

Mus musculus (house mouse)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.64 Å

Authors

Horton, J.R. / Ren, R. / Cheng, X.

Funding support

United States, 2items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM049245-23	United States
Cancer Prevention and Research Institute of Texas (CPRIT)	RR160029	United States

Citation

Journal: Biorxiv / Year: 2023
Title: The ICF syndrome protein CDCA7 harbors a unique DNA-binding domain that recognizes a CpG dyad in the context of a non-B DNA.
Authors: Hardikar, S. / Ren, R. / Ying, Z. / Horton, J.R. / Bramble, M.D. / Liu, B. / Lu, Y. / Liu, B. / Dan, J. / Zhang, X. / Cheng, X. / Chen, T.

History

Deposition	Jul 26, 2023	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 21, 2024	Provider: repository / Type: Initial release
Revision 1.1	Aug 28, 2024	Group: Database references / Category: citation / citation_author Item: _citation.journal_abbrev / _citation.journal_id_ISSN ..._citation.journal_abbrev / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8tlf.cif.gz	199.3 KB	Display	PDBx/mmCIF format
PDB format	pdb8tlf.ent.gz	126.4 KB	Display	PDB format
PDBx/mmJSON format	8tlf.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8tlf_validation.pdf.gz	461.3 KB	Display	wwPDB validaton report
Full document	8tlf_full_validation.pdf.gz	462.8 KB	Display
Data in XML	8tlf_validation.xml.gz	14.1 KB	Display
Data in CIF	8tlf_validation.cif.gz	20.3 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/tl/8tlf ftp://data.pdbj.org/pub/pdb/validation_reports/tl/8tlf	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
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PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: Cell division cycle-associated protein 7

B: Cell division cycle-associated protein 7

X: ssDNA

Y: ssDNA

hetero molecules

56 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Cell division cycle-associated protein 7

X: ssDNA

hetero molecules

A: Cell division cycle-associated protein 7

X: ssDNA

hetero molecules

defined by author&software
56.3 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Cell division cycle-associated protein 7

Y: ssDNA

hetero molecules

B: Cell division cycle-associated protein 7

Y: ssDNA

hetero molecules

defined by author&software
55.7 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	89.400, 31.492, 133.324
Angle α, β, γ (deg.)	90.000, 95.750, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y
Symmetry operation	#1: x,y,z #2: -x,y,-z #3: x+1/2,y+1/2,z #4: -x+1/2,y+1/2,-z

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Protein / DNA chain , 2 types, 4 molecules ABXY

#1: Protein	Cell division cycle-associated protein 7 Mass: 16452.814 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cdca7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-codon-plus (DE3)-RIL / References: UniProt: Q9D0M2
#2: DNA chain	ssDNA Mass: 11095.103 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: n vitro SELEX screening / Source: (synth.) Mus musculus (house mouse)

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Non-polymers , 5 types, 262 molecules

#3: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₃H₈O₃
#4: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL Mass: 62.068 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₂H₆O₂
#5: Chemical	ChemComp-ZN / ZINC ION Mass: 65.409 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Formula: Zn
#6: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 247 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 1.69 Å³/Da / Density % sol: 27.42 %
Crystal grow	Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.5 / Details: 0.2 M MgCl2, 0.1 M Tris pH8.5, 25%PEG3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 6, 2020
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 1.64→29.9 Å / Num. obs: 72067 / % possible obs: 89.9 % / Redundancy: 3.9 % / B_iso Wilson estimate: 17.1 Å² / CC_1/2: 0.992 / R_merge(I) obs: 0.095 / R_pim(I) all: 0.049 / Net I/σ(I): 13.6
Reflection shell	Resolution: 1.64→1.71 Å / Redundancy: 2.1 % / R_merge(I) obs: 0.509 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 4182 / CC_1/2: 0.867 / R_pim(I) all: 0.343 / % possible all: 65.4

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
SERGUI	1.20.1_4487	data collection
HKL-2000		data reduction
HKL-2000		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.64→29.9 Å / SU ML: 0.1698 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 26.0218
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2184	3484	4.84 %
R_work	0.1916	68499	-
obs	0.1929	71983	81.83 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 29.61 Å²

Refinement step

Cycle: LAST / Resolution: 1.64→29.9 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	1668	1052	34	247	3001

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0023	2906
X-RAY DIFFRACTION	f_angle_d	0.477	4141
X-RAY DIFFRACTION	f_chiral_restr	0.0358	446
X-RAY DIFFRACTION	f_plane_restr	0.0051	364
X-RAY DIFFRACTION	f_dihedral_angle_d	19.5252	1187

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.64-1.67	0	0	0.2958	1249	X-RAY DIFFRACTION	35.63
1.67-1.69	0.3159	138	0.2903	1597	X-RAY DIFFRACTION	49.12
1.69-1.72	0.2874	147	0.2785	1791	X-RAY DIFFRACTION	55.67
1.72-1.74	0	0	0.2803	2236	X-RAY DIFFRACTION	62.27
1.74-1.77	0.2752	153	0.28	2117	X-RAY DIFFRACTION	65.23
1.77-1.8	0.3117	161	0.2844	2287	X-RAY DIFFRACTION	70.04
1.8-1.83	0.2545	167	0.266	2480	X-RAY DIFFRACTION	74.69
1.83-1.87	0	0	0.2562	2714	X-RAY DIFFRACTION	77.68
1.87-1.91	0.3111	167	0.2363	2530	X-RAY DIFFRACTION	76.64
1.91-1.95	0.2565	172	0.2319	2677	X-RAY DIFFRACTION	80.37
1.95-1.99	0.2342	175	0.2324	2677	X-RAY DIFFRACTION	81.44
1.99-2.04	0.2736	179	0.2376	2981	X-RAY DIFFRACTION	89.77
2.04-2.1	0.2712	180	0.2102	3128	X-RAY DIFFRACTION	92.9
2.1-2.16	0	0	0.2217	3317	X-RAY DIFFRACTION	94.77
2.16-2.23	0.2507	190	0.2184	3139	X-RAY DIFFRACTION	95.36
2.23-2.31	0.2393	189	0.2035	3106	X-RAY DIFFRACTION	94.63
2.31-2.4	0.26	185	0.2025	3253	X-RAY DIFFRACTION	95.74
2.4-2.51	0.2335	179	0.1969	3062	X-RAY DIFFRACTION	92.79
2.51-2.65	0.2368	186	0.2071	3132	X-RAY DIFFRACTION	93.49
2.65-2.81	0.2168	185	0.1981	2949	X-RAY DIFFRACTION	89.77
2.81-3.03	0	0	0.2025	3326	X-RAY DIFFRACTION	95.88
3.03-3.33	0.1989	189	0.1778	3239	X-RAY DIFFRACTION	96.75
3.33-3.81	0.1892	184	0.1479	3241	X-RAY DIFFRACTION	96.83
3.81-4.8	0.1692	178	0.1364	3148	X-RAY DIFFRACTION	94.92
4.8-29.9	0.1552	180	0.1519	3123	X-RAY DIFFRACTION	93.41

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.312415525633	0.567569533353	0.360640601329	1.42431928962	-0.658053127071	5.32168052956	-0.402732460657	0.0967963337736	0.629937275651	-0.101991105167	0.145918735755	-0.606434477796	-0.488641050018	0.539883754154	0.300881385862	0.298612778512	-0.046105796762	-0.0986591640325	0.359225481793	-0.0259049000813	0.600885149186	19.1996542868	7.74087766812	13.3578263393
2	8.21318143911	-2.74001261263	1.01160978584	5.63626904722	-1.15338128847	1.80632086781	-0.259637288027	-0.335312528905	-0.246431433744	0.990703611129	0.257892174552	-0.259109851381	-0.110819824011	0.167746530772	-0.0114390317716	0.237196282193	0.0184001558161	-0.0620732928706	0.170110641511	-0.0506495333495	0.166677774458	9.14201362214	5.11415630317	26.9930543874
3	1.92863310801	-0.841669420443	-0.00248280609077	1.65810100573	0.823272838136	2.21642179276	-0.0341725180198	0.00383893818109	0.189063149931	0.056222459671	0.111713117582	-0.333128537224	-0.113918712716	0.206989224682	-0.0645050595554	0.127833985832	0.0160479219498	-0.0204756144044	0.085644059546	-0.0244736442535	0.146566457528	8.98488851178	4.62991517323	17.9029909056
4	5.6913665872	-3.94237508756	2.05448893045	5.12322106841	-2.67562068415	1.39689056371	0.253179605331	0.967308179597	-0.303337223632	-0.887067157122	-0.29143699563	-0.423404532403	0.0717701733788	1.01983612566	-0.0228194224085	0.220491543754	0.0231872075501	0.0870358485094	0.382639395631	-0.0346480598019	0.243854700259	16.7361891053	0.424664803568	3.19005337538
5	1.46948340767	-1.09443266863	1.63612275299	4.00222136196	-4.8587897696	6.74420775365	-0.136176765592	0.0775234038086	0.141821506308	-0.204651088901	0.0314580951548	-0.0374437536925	-0.36806954304	-0.230502302472	0.131961514536	0.189882896808	0.028349793687	-0.0258141725017	0.157794053456	-0.0117081757661	0.165795460774	9.56670187882	1.40814092308	10.5561992352
6	4.54389829743	2.93191831481	-0.878269865887	5.80703465053	3.00315611236	3.58435992881	0.358983647738	-0.139117067166	-0.14326983092	0.491558031206	-0.0845596445757	-0.573162225507	0.396201736941	0.207018924191	-0.251709177748	0.181851567804	0.0437343325051	-0.0601282138857	0.12795578237	-0.0106422885148	0.217477888272	10.9081013642	-6.63839973812	21.7011109289
7	3.16065800579	3.71069834676	-1.7003588043	7.10655822297	0.0952088473295	2.49086248037	0.361620592948	-0.238200202977	-0.494938644333	0.645225913731	-0.148615200022	-1.00703065953	0.344000240203	0.678953026273	-0.232523597044	0.188241533538	0.0769930393901	-0.0792623765734	0.245989172218	-0.0420836401202	0.338444666797	18.6584248175	-5.28605323895	18.0900524459
8	6.86102296444	1.655088	0.722359897395	1.30406753656	-0.760616798203	1.10045941126	-0.100501188758	0.536865814897	-0.482360533559	-0.205896012235	0.243627161329	-0.633558932426	0.441107532069	0.566780473259	-0.0211671280482	0.156981038626	0.0519747653228	0.0309384430327	0.263873290078	-0.0986241340379	0.293547837584	15.3738473208	-5.91503561703	9.46185878313
9	8.86346044569	-2.32216844823	-1.54336767447	6.9905634282	-0.086308569388	2.34974554665	-0.0058625111243	1.27181862087	-0.710736937378	-0.98761266507	0.105421218655	1.36194089194	1.10775027842	-1.31744094993	-0.0497407341008	0.313027769248	-0.087723925903	-0.0770321383394	0.31365106309	-0.0566418935109	0.32619965837	-0.813508335759	-7.72120831396	10.7694020288
10	1.99413595035	1.59001842885	0.235961820516	5.19854393603	-1.02526570485	4.25073180533	-0.247564388422	-0.592204188599	0.653840365751	0.347102875489	-0.0469829222629	0.428879697019	-0.749290188724	-0.367998020871	0.293001270299	0.423685620615	0.134740250092	0.032623584102	0.387673907684	-0.109049620026	0.369622774893	-19.7582614661	5.64486676907	60.7404187402
11	6.29245292074	-2.40293846892	-1.82385627475	7.80070895006	1.32960122618	5.34990506061	0.423158328868	0.169309797731	0.96001596982	-0.0719944883716	-0.0308376490389	1.20290345502	-0.74110873666	-1.65564585969	-0.456953766141	0.274021754477	0.0795113894557	-0.0284528917194	0.694582258374	-0.0923732183168	0.836423992438	-33.1271831832	3.98921312303	48.0425502911
12	9.3482254757	2.51096640237	0.190803868719	5.39020927249	1.87892167121	6.83492650426	-0.216362790589	0.576348270596	-0.34724609497	-0.850832053991	0.211883370308	0.344083280152	-0.292510105923	-0.168158325282	0.0141031920977	0.254255228914	0.0244255521561	-0.0161699866122	0.186314809134	0.012950252249	0.202365348412	-16.9345696053	3.05025966197	40.1813955217
13	3.26388643982	0.283171630518	0.952027880898	4.19490016391	-0.871350592077	2.92570490349	-0.0767589444172	0.0580258072371	0.123389198267	-0.00525214952301	0.0341111155897	0.238438444992	-0.348073336493	-0.196608878904	0.0162523138631	0.182849257373	0.0103838081849	0.0392626769215	0.109863747237	0.00402436731329	0.126858134748	-16.7102714218	1.69638401318	48.94554978
14	7.32677043912	1.67622203499	1.67655073894	6.65901230884	1.5257122424	0.594248341151	0.124856138014	-0.481618741416	-0.202631838585	0.576839767895	-0.394790026234	0.058799186638	0.145457745501	-0.848710762697	0.1680950014	0.286586972049	0.0388992199149	0.203010826287	0.448999481813	0.00192618526548	0.367714637298	-23.6574422889	-2.86614951867	63.9408988892
15	1.67292520695	1.74071623525	2.16218491742	2.94044288984	3.18307287621	3.55702092548	-0.260815860039	-0.13096836352	0.0435451482784	0.146193528571	-0.0360158200027	0.0432406156103	0.233646513343	0.221246331652	0.261883285395	0.259977258872	0.0204942591733	0.0631001632528	0.201638825882	-0.0106159156425	0.181784847696	-16.8684459423	-1.71730033461	56.2532047543
16	4.70070477444	-2.00430668294	0.644637248764	2.00347715771	-2.16259520639	8.09775570509	0.258029817189	0.187797084036	-0.241077204842	-0.286366431122	-0.00904838701897	0.533990154719	0.309977074685	-0.559723690813	-0.171770912277	0.147717474487	-0.00894979982009	0.00713680276579	0.183336094621	-0.0397604202838	0.248998413379	-18.9262063405	-9.42711707031	44.9914679064
17	4.45666691696	-5.48236127772	-0.953456671029	6.78126168554	0.883999401319	2.07946049416	0.136619555607	0.0771381297709	-0.51612886641	0.14704021666	-0.0432370625555	1.28579300236	0.234158421512	-0.842059635441	-0.0978050715046	0.175717054944	-0.0407216222369	0.0510450609907	0.342064251311	0.0257436842344	0.417730532111	-26.4080184	-8.0478594238	49.0066446197
18	4.35930119146	-1.42467570394	1.0684556344	3.43441622157	0.829602525662	0.735653134513	-0.124235544146	-0.377485577562	-0.285425446648	0.635590593913	0.135447867992	0.555458819932	0.530700090496	-0.745186254391	-0.0455785773644	0.2674257681	-0.0470436422587	0.101417927023	0.35711369662	0.0527398925514	0.264001293456	-21.9690490229	-9.04516574485	57.1507826159
19	4.11557070969	3.10760332335	2.16836002948	2.76520585169	0.417349794708	4.75281436751	0.358089450257	-1.45181420291	-1.2454293151	1.34626449812	0.143345786295	-1.37625855298	2.15669365962	1.65663965454	-0.527889704889	0.632518848473	0.164677251711	-0.141250169654	0.566655558128	0.0372667656603	0.486615026808	-5.77328491471	-11.2344851006	56.5405587669
20	8.96200510449	-4.99832600364	-2.82617832411	9.36141196345	5.75120539678	3.57750790396	0.0405191046642	1.09844618705	-0.943906531896	-0.756567009189	-0.742961135823	1.27543969059	-0.0376069402498	-0.588128000114	0.681683145504	0.438018588446	0.0136594118201	-0.102407369121	0.41910753695	-0.0824603004435	0.32340805237	-5.99583725848	-15.0279402076	-35.787075444
21	0.915594182615	-0.750647019768	-1.12430444573	1.29887874896	0.484782481656	2.40588658531	0.0612831706296	-0.229898624625	-0.0408592039481	0.096869694329	0.0587776445078	-0.0768817519005	0.098345943976	0.317155060974	-0.106397997228	0.138173387628	-0.0191160171716	-0.00280061542489	0.167795843424	0.0322597540924	0.0867408546931	4.13419216348	1.04108522215	-12.5458314723
22	0.704628381732	0.067919747352	-0.0513232372917	3.33882460583	-0.685765325747	0.438697570631	-0.0461779257292	-0.00487132314662	-0.0778038779539	0.251521244198	0.103701910308	0.112956658206	0.041585555271	-0.0955973026301	-0.0204162911954	0.174825355739	0.0173704202357	-0.00110898263476	0.152901902132	-0.0192031903732	0.132747681805	-2.40062220798	-3.4522818467	23.4061196925
23	0.537605267805	0.0354638146258	0.412530280795	1.79494063258	-2.06873127644	5.28253526673	0.0781886822792	0.0265192854486	-0.204486357452	-0.0903365406769	0.0623958508107	0.00912840173329	0.0378270323733	-0.190991394648	-0.0932977051107	0.128307982838	0.0032084656755	0.0473764642002	0.210005766958	0.0116405715296	0.147718385698	-6.24937534812	-6.44275920548	86.9851899352
24	0.971424254263	-0.137627461333	-0.320702676932	3.79530117178	2.1669881618	4.81638024501	-0.123800184746	0.174408245847	-0.193678610861	-0.0806617746464	0.199167942613	-0.269385338209	-0.0663262574263	0.515641166146	-0.052535255542	0.169795268392	-0.0207285510792	0.0400152363657	0.262951954148	-0.0664452351	0.18002345506	-5.4418869062	-4.44861463778	42.4850741767

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 243 through 262 )	A	A	243 - 262	1 - 20
2	2	chain 'A' and (resid 263 through 275 )	A	A	263 - 275	21 - 33
3	3	chain 'A' and (resid 276 through 295 )	A	A	276 - 295	34 - 53
4	4	chain 'A' and (resid 296 through 300 )	A	A	296 - 300	54 - 58
5	5	chain 'A' and (resid 301 through 308 )	A	A	301 - 308	59 - 66
6	6	chain 'A' and (resid 309 through 315 )	A	A	309 - 315	67 - 73
7	7	chain 'A' and (resid 316 through 324 )	A	A	316 - 324	74 - 82
8	8	chain 'A' and (resid 325 through 339 )	A	A	325 - 339	83 - 97
9	9	chain 'A' and (resid 340 through 346 )	A	A	340 - 346	98 - 104
10	10	chain 'B' and (resid 243 through 252 )	B	D	243 - 252	1 - 10
11	11	chain 'B' and (resid 253 through 262 )	B	D	253 - 262	11 - 20
12	12	chain 'B' and (resid 263 through 275 )	B	D	263 - 275	21 - 33
13	13	chain 'B' and (resid 276 through 295 )	B	D	276 - 295	34 - 53
14	14	chain 'B' and (resid 296 through 300 )	B	D	296 - 300	54 - 58
15	15	chain 'B' and (resid 301 through 308 )	B	D	301 - 308	59 - 66
16	16	chain 'B' and (resid 309 through 315 )	B	D	309 - 315	67 - 73
17	17	chain 'B' and (resid 316 through 324 )	B	D	316 - 324	74 - 82
18	18	chain 'B' and (resid 325 through 340 )	B	D	325 - 340	83 - 98
19	19	chain 'B' and (resid 341 through 346 )	B	D	341 - 346	99 - 104
20	20	chain 'X' and (resid 7 through 11 )	X	E	7 - 11
21	21	chain 'X' and (resid 12 through 21 )	X	E	12 - 21
22	22	chain 'X' and (resid 22 through 32 )	X	E	22 - 32
23	23	chain 'Y' and (resid 7 through 21 )	Y	H	7 - 21
24	24	chain 'Y' and (resid 22 through 32 )	Y	H	22 - 32

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