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Open data
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Basic information
Entry | Database: PDB / ID: 8thy | ||||||
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Title | Yeast actin A167E mutant rabbit-like | ||||||
![]() | Actin | ||||||
![]() | CYTOSOLIC PROTEIN / actin filaments / cytoskeleton / cations / stiffness | ||||||
Function / homology | ![]() cellular bud neck contractile ring / fungal biofilm matrix / ascospore wall assembly / mitotic actomyosin contractile ring contraction / vacuole inheritance / actin cortical patch / Swr1 complex / Ino80 complex / establishment of cell polarity / NuA4 histone acetyltransferase complex ...cellular bud neck contractile ring / fungal biofilm matrix / ascospore wall assembly / mitotic actomyosin contractile ring contraction / vacuole inheritance / actin cortical patch / Swr1 complex / Ino80 complex / establishment of cell polarity / NuA4 histone acetyltransferase complex / actin filament bundle / protein secretion / actin filament / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / endocytosis / chromatin remodeling / hydrolase activity / DNA repair / extracellular region / ATP binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.84 Å | ||||||
![]() | Volkmann, N. / Hanein, D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: yeast actin A167E mutant rabbit-like Authors: Volkmann, N. / Hanein, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 319.2 KB | Display | ![]() |
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PDB format | ![]() | 263.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 41274MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 41231.973 Da / Num. of mol.: 5 / Mutation: A167E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P60009, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement #2: Chemical | ChemComp-ADP / #3: Chemical | ChemComp-MG / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: yeast actin A167E mutant filament / Type: COMPLEX / Entity ID: #1 / Source: NATURAL |
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Source (natural) | Organism: ![]() ![]() |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2800 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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Helical symmerty | Angular rotation/subunit: -167.25 ° / Axial rise/subunit: 27.49 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.84 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 195534 / Symmetry type: HELICAL | ||||||||||||||||||||||||
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