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Open data
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Basic information
Entry | Database: PDB / ID: 8thn | ||||||
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Title | KcsA M96V mutant with Y78ester in High K+ | ||||||
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![]() | MEMBRANE PROTEIN / Ion transport / Potassium ion channel / ester | ||||||
Function / homology | delayed rectifier potassium channel activity / Potassium channel domain / Ion channel / voltage-gated potassium channel complex / identical protein binding / : / TETRABUTYLAMMONIUM ION / pH-gated potassium channel KcsA![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Reddi, R. / Valiyaveetil, F.I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A facile approach for incorporating tyrosine esters to probe ion-binding sites and backbone hydrogen bonds. Authors: Reddi, R. / Chatterjee, S. / Matulef, K. / Gustafson, A. / Gao, L. / Valiyaveetil, F.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.2 KB | Display | ![]() |
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PDB format | ![]() | 87.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.1 KB | Display | ![]() |
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Full document | ![]() | 454.7 KB | Display | |
Data in XML | ![]() | 19.7 KB | Display | |
Data in CIF | ![]() | 26.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8dhrC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 10957.671 Da / Num. of mol.: 1 / Mutation: M96V, Y78ester Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Non-polymers , 3 types, 6 molecules ![](data/chem/img/K.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-K / #5: Chemical | ChemComp-TBA / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.96 Å3/Da / Density % sol: 68.97 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.25 Details: 27% PEG 400 (v/v), 50 mM magnesium acetate, 50 mM MES pH 6.25 PH range: 6.0- 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 20, 2018 |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→55.01 Å / Num. obs: 20234 / % possible obs: 99.9 % / Redundancy: 6.3 % / CC1/2: 0.98 / Net I/σ(I): 5.1 |
Reflection shell | Resolution: 2.9→3.08 Å / Num. unique obs: 3264 / CC1/2: 0.365 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→55.01 Å
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Refine LS restraints |
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LS refinement shell |
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