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- PDB-8t01: Solution NMR structure of a multifunctional synthetic peptide Hp-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8t01 | ||||||
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Title | Solution NMR structure of a multifunctional synthetic peptide Hp-MAP3 derived from Temporin-PTa | ||||||
![]() | Hp-MAP3 peptide | ||||||
![]() | ANTIMICROBIAL PROTEIN | ||||||
Function / homology | defense response to bacterium / extracellular region / Temporin-PTa![]() | ||||||
Biological species | Pulchrana picturata (frog) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Freitas, C.D.P. / Migliolo, L. / Silva, P.S. / Liao, L.M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Solution NMR structure of a multifunctional synthetic peptide Hp-MAP3 derived from Temporin-PTa. Authors: Freitas, C.D.P. / Migliolo, L. / Silva, P.S. / Liao, L.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.3 KB | Display | ![]() |
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PDB format | ![]() | 29.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 486.7 KB | Display | ![]() |
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Full document | ![]() | 597 KB | Display | |
Data in XML | ![]() | 13.5 KB | Display | |
Data in CIF | ![]() | 15.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1483.021 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The Hp-MAP3 peptide is an analog derived from Temporin-PTa. The primary sequence of the parent peptide was used as a template for rational design, using amino acid residues for charge ...Details: The Hp-MAP3 peptide is an analog derived from Temporin-PTa. The primary sequence of the parent peptide was used as a template for rational design, using amino acid residues for charge modification and hydrophobicity. Thus obtaining an amphipathic peptide. Source: (synth.) Pulchrana picturata (frog) / References: UniProt: P0C8U2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 1.5 mM Hp-MAP3 (peptide), 75 mM [U-98% 2H] SDS-d25, 0.05 % [U-98% 2H] DSS-D6, 90% H2O/10% D2O Label: Hp-MAP3 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: neutral Not defined / Label: 1 / pH: 6.8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 9 |