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Open data
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Basic information
Entry | Database: PDB / ID: 8sy8 | ||||||
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Title | Crystal structure of TsaC | ||||||
![]() | 4-formylbenzenesulfonate dehydrogenase TsaC | ||||||
![]() | OXIDOREDUCTASE / Short chain dehydrogenase/reductase / Rossmann fold / NAD(H)binding | ||||||
Function / homology | 4-formylbenzenesulfonate dehydrogenase / 4-formylbenzenesulfonate dehydrogenase activity / : / Short-chain dehydrogenase/reductase, conserved site / Short-chain dehydrogenases/reductases family signature. / Enoyl-(Acyl carrier protein) reductase / Short-chain dehydrogenase/reductase SDR / NAD(P)-binding domain superfamily / 4-formylbenzenesulfonate dehydrogenase TsaC1/TsaC2![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Boggs, D.G. / Tian, J. / Bridwell-Rabb, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The NADH recycling enzymes TsaC and TsaD regenerate reducing equivalents for Rieske oxygenase chemistry. Authors: Tian, J. / Boggs, D.G. / Donnan, P.H. / Barroso, G.T. / Garcia, A.A. / Dowling, D.P. / Buss, J.A. / Bridwell-Rabb, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.7 KB | Display | ![]() |
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PDB format | ![]() | 69.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.6 KB | Display | ![]() |
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Full document | ![]() | 449.2 KB | Display | |
Data in XML | ![]() | 17.5 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28739.502 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P94681, 4-formylbenzenesulfonate dehydrogenase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.81 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: 0.2 M KCl, 20% polyethylene glycol 3350, and 10% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Nov 1, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97872 Å / Relative weight: 1 |
Reflection | Resolution: 2.18→43.59 Å / Num. obs: 26991 / % possible obs: 99.94 % / Redundancy: 16.68 % / Biso Wilson estimate: 54.61 Å2 / CC1/2: 1 / Rrim(I) all: 0.072 / Net I/σ(I): 24.18 |
Reflection shell | Resolution: 2.18→2.32 Å / Num. unique obs: 2670 / CC1/2: 0.72 / Rrim(I) all: 1.5 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.28 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.18→43.59 Å
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Refine LS restraints |
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LS refinement shell |
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