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Open data
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Basic information
Entry | Database: PDB / ID: 8sxm | ||||||
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Title | NMR structure of the ZNF750 zinc finger domain, Z* | ||||||
![]() | Zinc finger protein 750 | ||||||
![]() | METAL BINDING PROTEIN / degenerate zinc finger / ZNF750 / ZN750 / nuclear transcription factor / protein structure evolution / somatic cancer mutations / psoriasis / skin differentiation / DNA-binding motif / CCHC zinc finger / antiparallel bba motif / protein folding / protein structure prediction / seborrhea-like dermatosis with non-arthritic psoriasiform elements / SLDP | ||||||
Function / homology | ![]() Generic Transcription Pathway / negative regulation of epithelial to mesenchymal transition / epidermis development / promoter-specific chromatin binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / DNA-binding transcription activator activity, RNA polymerase II-specific / cell differentiation / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II ...Generic Transcription Pathway / negative regulation of epithelial to mesenchymal transition / epidermis development / promoter-specific chromatin binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / DNA-binding transcription activator activity, RNA polymerase II-specific / cell differentiation / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Rua, A.J. / Alexandrescu, A.T. | ||||||
Funding support | 1items
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![]() | ![]() Title: NMR structure verifies the eponymous zinc finger domain of transcription factor ZNF750. Authors: Rua, A.J. / Whitehead 3rd, R.D. / Alexandrescu, A.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 180.4 KB | Display | ![]() |
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PDB format | ![]() | 148.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 536.9 KB | Display | ![]() |
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Full document | ![]() | 777.5 KB | Display | |
Data in XML | ![]() | 28.1 KB | Display | |
Data in CIF | ![]() | 38.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3314.924 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
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#2: Chemical | ChemComp-ZN / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz / Details: TCI cryogenic probe |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 Details: Structures are based on 330 NOE restraints, 12 (x2) hydrogen bonds, 4 restraints to the zinc ion, and 60 dihedral angles. | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |