+Open data
-Basic information
Entry | Database: PDB / ID: 8sx0 | ||||||
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Title | Bordetella filamentous hemagglutinin (FhaB) C-terminal domain | ||||||
Components | Filamentous hemagglutinin | ||||||
Keywords | TOXIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Bordetella pertussis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.65 Å | ||||||
Authors | Cuthbert, B.J. / Goulding, C.W. / Hayes, C.S. / Costello, M.S. | ||||||
Funding support | United States, 1items
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Citation | Journal: To Be Published Title: Bordetella filamentous hemagglutinin (FhaB) C-terminal domain Authors: Cuthbert, B.J. / Goulding, C.W. / Hayes, C.S. / Costello, M.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8sx0.cif.gz | 110.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8sx0.ent.gz | 69.4 KB | Display | PDB format |
PDBx/mmJSON format | 8sx0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8sx0_validation.pdf.gz | 416.3 KB | Display | wwPDB validaton report |
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Full document | 8sx0_full_validation.pdf.gz | 417.4 KB | Display | |
Data in XML | 8sx0_validation.xml.gz | 5.8 KB | Display | |
Data in CIF | 8sx0_validation.cif.gz | 8.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sx/8sx0 ftp://data.pdbj.org/pub/pdb/validation_reports/sx/8sx0 | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12771.240 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bordetella pertussis (bacteria) / Gene: fhaB_1, NCTC10911_02131 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A381A265 #2: Chemical | #3: Chemical | #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.6 Å3/Da / Density % sol: 22.98 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 0.1 M phosphate citrate pH 5, 26.5% PEG 8000, 0.2 M NaCl Temp details: cold room |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 0.9795, 1.0 | ||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 28, 2022 | ||||||||||||||||||||||||
Radiation | Monochromator: Double-crystal, Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength |
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Reflection | Entry-ID: 8SX0 / CC1/2: 0.999 / Rpim(I) all: 0.038 / Χ2: 0.99 / Diffraction-ID: 1
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Reflection shell | Resolution: 1.65→1.68 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.805 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 962 / CC1/2: 0.704 / Rpim(I) all: 0.501 / Rrim(I) all: 0.952 / Χ2: 0.98 / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.65→39.71 Å / SU ML: 0.1898 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.2807 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.44 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→39.71 Å
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Refine LS restraints |
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LS refinement shell |
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