TRANSFERASE / SSGCID / STRUCTURAL GENOMICS / SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE / OXIDOREDUCTASE / ArnB Transferase
機能・相同性
機能・相同性情報
UDP-4-amino-4-deoxy-L-arabinose aminotransferase / UDP-4-amino-4-deoxy-L-arabinose aminotransferase / lipopolysaccharide biosynthetic process / lipid A biosynthetic process / pyridoxal phosphate binding / response to antibiotic / metal ion binding 類似検索 - 分子機能
UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase, ArnB / DegT/DnrJ/EryC1/StrS aminotransferase / DegT/DnrJ/EryC1/StrS aminotransferase family / Pyridoxal phosphate-dependent transferase, small domain / Pyridoxal phosphate-dependent transferase, major domain / Pyridoxal phosphate-dependent transferase 類似検索 - ドメイン・相同性
構造決定の手法: 分子置換 / 解像度: 1.55→58.82 Å / SU ML: 0.19 / 交差検証法: FREE R-VALUE / σ(F): 1.33 / 位相誤差: 27.82 / 立体化学のターゲット値: ML 詳細: Lattice-translocation disorder correction notes: Native Patterson shows three off-origin peaks. Strongest one with vector (0.4029 0.50000.618), second one (0.1953 0.000 0.7638), and third one ...詳細: Lattice-translocation disorder correction notes: Native Patterson shows three off-origin peaks. Strongest one with vector (0.4029 0.50000.618), second one (0.1953 0.000 0.7638), and third one at (0.2092 0.5000 0.8536), corresponding to 1X Td, 2X Td and 3X Td, respectively, with relative intensities to orgin peak of ~65%, ~30% and 8.5% (CCP4 fft used; phenix.xtriage showed even higher values like 73%, 34% and 10%, most likely due to grid sampling difference), respectively. Peak 1 and 2 are mostly targeted. Peak 1 corresponds to tNCS too, so after correction it is lowered but not eliminated. Peak 2 eventually gone. Final Rwork and Rfee went down to 19.81% and 21.99%,respectively. Without correction, the Rwork and Rfree, with best try, are paused at 31.38% and 33.83%, respectively.
Rfactor
反射数
%反射
Rfree
0.2199
5787
5 %
Rwork
0.1981
-
-
obs
0.1992
115703
99.65 %
溶媒の処理
減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL