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- PDB-8slv: Structure of a salivary alpha-glucosidase from the mosquito vecto... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8slv | ||||||
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Title | Structure of a salivary alpha-glucosidase from the mosquito vector Aedes aegypti. | ||||||
![]() | Salivary alpha-glucosidase | ||||||
![]() | HYDROLASE / Salivary alpha-glucosidase / mosquito | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gittis, A.G. / Williams, A.E. / Garboczi, D. / Calvo, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional comparisons of salivary alpha-glucosidases from the mosquito vectors Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus. Authors: Williams, A.E. / Gittis, A.G. / Botello, K. / Cruz, P. / Martin-Martin, I. / Valenzuela Leon, P.C. / Sumner, B. / Bonilla, B. / Calvo, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 149.2 KB | Display | ![]() |
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PDB format | ![]() | 103.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 508.5 KB | Display | ![]() |
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Full document | ![]() | 512.6 KB | Display | |
Data in XML | ![]() | 25.5 KB | Display | |
Data in CIF | ![]() | 37.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein / Sugars , 2 types, 2 molecules A![](data/chem/img/NAG.gif)
![](data/chem/img/NAG.gif)
#1: Protein | Mass: 66760.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Sugar | ChemComp-NAG / |
-Non-polymers , 8 types, 342 molecules ![](data/chem/img/CYS.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/IPA.gif)
![](data/chem/img/PDO.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/IPA.gif)
![](data/chem/img/PDO.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-CYS / | ||||||||||||
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#4: Chemical | ChemComp-GOL / #5: Chemical | ChemComp-TRS / | #6: Chemical | ChemComp-IPA / | #7: Chemical | ChemComp-PDO / | #8: Chemical | #9: Chemical | ChemComp-CL / #10: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.84 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 0.2 M magnesium chloride hexahydrate, , 0.1 M HEPES sodium pH 7.5, 30% v/v 2-propanol |
-Data collection
Diffraction | Mean temperature: 95.5 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: DECTRIS EIGER2 R 4M / Detector: PIXEL / Date: Jul 29, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 2.32→24.79 Å / Num. obs: 27047 / % possible obs: 94.8 % / Redundancy: 3.2 % / Biso Wilson estimate: 19.86 Å2 / CC1/2: 0.99 / Rrim(I) all: 0.039 / Net I/σ(I): 26.95 |
Reflection shell | Resolution: 2.32→2.38 Å / Redundancy: 2.88 % / Mean I/σ(I) obs: 11.82 / Num. unique obs: 1623 / CC1/2: 0.98 / Rrim(I) all: 0.106 / % possible all: 78.4 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.48 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.32→22.55 Å
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Refine LS restraints |
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LS refinement shell |
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