- PDB-8s5b: Crystal structure of the sulfoquinovosyl binding protein (SmoF) f... -
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Basic information
Entry
Database: PDB / ID: 8s5b
Title
Crystal structure of the sulfoquinovosyl binding protein (SmoF) from A. tumefaciens sulfo-SMO pathway in complex with SQOctyl ligand
Components
Sulfoquinovosyl glycerol-binding protein SmoF
Keywords
SUGAR BINDING PROTEIN / agrobacterium tumefaciens / sulfoquinovose / sulfosugar / solute binding protein / sulfoglycolysis / sulfo-SMO pathway
Function / homology
carbohydrate transport / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / periplasmic space / : / Sulfoquinovosyl glycerol-binding protein SmoF
Mass: 18.015 Da / Num. of mol.: 243 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.15 Å3/Da / Density % sol: 42.78 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 200 mM calcium acetate, 100 mM Bis-TRIS pH 6.5, 25% w/v PEG 2000 MME, 1:1 protein:mother liquor ratio with SmoF at 50mg/ml in 50mM TRIS, 300mM NaCl pH 7.5
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Data collection
Diffraction
Mean temperature: 120 K / Serial crystal experiment: N
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