+Open data
-Basic information
Entry | Database: PDB / ID: 8s1m | ||||||
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Title | Crystal structure of human GABARAP fused to EGFR (1076-1099) | ||||||
Components | Epidermal growth factor receptor,Gamma-aminobutyric acid receptor-associated protein | ||||||
Keywords | PROTEIN BINDING / Atg8 / Receptor-trafficking | ||||||
Function / homology | Function and homology information positive regulation of protein K48-linked ubiquitination / regulation of Rac protein signal transduction / GABA receptor binding / phosphatidylethanolamine binding / TBC/RABGAPs / response to hydroxyisoflavone / multivesicular body, internal vesicle lumen / positive regulation of protein kinase C activity / positive regulation of prolactin secretion / negative regulation of cardiocyte differentiation ...positive regulation of protein K48-linked ubiquitination / regulation of Rac protein signal transduction / GABA receptor binding / phosphatidylethanolamine binding / TBC/RABGAPs / response to hydroxyisoflavone / multivesicular body, internal vesicle lumen / positive regulation of protein kinase C activity / positive regulation of prolactin secretion / negative regulation of cardiocyte differentiation / diterpenoid metabolic process / Shc-EGFR complex / cellular response to nitrogen starvation / ovulation cycle / tongue development / Inhibition of Signaling by Overexpressed EGFR / epidermal growth factor receptor activity / EGFR interacts with phospholipase C-gamma / microtubule associated complex / positive regulation of mucus secretion / epidermal growth factor binding / response to UV-A / PLCG1 events in ERBB2 signaling / midgut development / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / hydrogen peroxide metabolic process / ERBB2-EGFR signaling pathway / PTK6 promotes HIF1A stabilization / digestive tract morphogenesis / morphogenesis of an epithelial fold / ERBB2 Activates PTK6 Signaling / intracellular vesicle / Macroautophagy / Signaling by EGFR / transmembrane receptor protein tyrosine kinase activator activity / protein tyrosine kinase activator activity / negative regulation of epidermal growth factor receptor signaling pathway / response to cobalamin / Signaling by ERBB4 / eyelid development in camera-type eye / beta-tubulin binding / protein insertion into membrane / cerebral cortex cell migration / ERBB2 Regulates Cell Motility / axoneme / Respiratory syncytial virus (RSV) attachment and entry / regulation of JNK cascade / autophagosome membrane / PI3K events in ERBB2 signaling / positive regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of mitotic cell cycle / autophagosome maturation / autophagosome assembly / extrinsic apoptotic signaling pathway via death domain receptors / hair follicle development / MAP kinase kinase kinase activity / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / embryonic placenta development / smooth endoplasmic reticulum / positive regulation of bone resorption / GAB1 signalosome / positive regulation of G1/S transition of mitotic cell cycle / protein targeting / salivary gland morphogenesis / peptidyl-tyrosine autophosphorylation / positive regulation of phosphorylation / regulation of peptidyl-tyrosine phosphorylation / positive regulation of glial cell proliferation / positive regulation of vasoconstriction / Signaling by ERBB2 / sperm midpiece / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / cellular response to epidermal growth factor stimulus / EGFR Transactivation by Gastrin / transmembrane receptor protein tyrosine kinase activity / cellular response to cadmium ion / GRB2 events in ERBB2 signaling / autophagosome / positive regulation of DNA repair / neurogenesis / SHC1 events in ERBB2 signaling / regulation of ERK1 and ERK2 cascade / cellular response to dexamethasone stimulus / ossification / positive regulation of synaptic transmission, glutamatergic / positive regulation of epithelial cell proliferation / neuron projection morphogenesis / epithelial cell proliferation / basal plasma membrane / positive regulation of superoxide anion generation / positive regulation of DNA replication / Signal transduction by L1 / cellular response to estradiol stimulus / liver regeneration / NOTCH3 Activation and Transmission of Signal to the Nucleus / astrocyte activation / positive regulation of protein localization to plasma membrane / cellular response to amino acid stimulus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å | ||||||
Authors | Ueffing, A. / Willbold, D. / Weiergraeber, O.H. | ||||||
Funding support | Germany, 1items
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Citation | Journal: To Be Published Title: Crystal structure of human GABARAP fused to EGFR (1076-1099) Authors: Ueffing, A. / Willbold, D. / Weiergraeber, O.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8s1m.cif.gz | 79 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8s1m.ent.gz | 49.3 KB | Display | PDB format |
PDBx/mmJSON format | 8s1m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8s1m_validation.pdf.gz | 354.8 KB | Display | wwPDB validaton report |
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Full document | 8s1m_full_validation.pdf.gz | 396.3 KB | Display | |
Data in XML | 8s1m_validation.xml.gz | 4.3 KB | Display | |
Data in CIF | 8s1m_validation.cif.gz | 6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s1/8s1m ftp://data.pdbj.org/pub/pdb/validation_reports/s1/8s1m | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Experimental dataset #1 | Data reference: 10.15151/ESRF-DC-1524662410 / Data set type: diffraction image data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16905.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: EGFR(1052-1075) fused to N-terminus of GABARAP,EGFR(1052-1075) fused to N-terminus of GABARAP,EGFR(1052-1075) fused to N-terminus of GABARAP,EGFR(1052-1075) fused to N-terminus of GABARAP Source: (gene. exp.) Homo sapiens (human) / Gene: EGFR, ERBB, ERBB1, HER1, GABARAP, FLC3B, HT004 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): T1 References: UniProt: P00533, UniProt: O95166, receptor protein-tyrosine kinase |
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#2: Chemical | ChemComp-CL / |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.96 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 0.17 M ammonium acetate 0.085 M sodium citrate 25.5% PEG 4000 15% Glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.9677 Å |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Sep 17, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→41.35 Å / Num. obs: 11007 / % possible obs: 100 % / Redundancy: 20.1 % / Biso Wilson estimate: 57.43 Å2 / CC1/2: 1 / Net I/σ(I): 21.91 |
Reflection shell | Resolution: 2.05→2.1 Å / Redundancy: 20.1 % / Mean I/σ(I) obs: 1.01 / Num. unique obs: 798 / CC1/2: 0.365 / % possible all: 99.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.05→41.35 Å / SU ML: 0.3497 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 33.7698 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 72.05 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.05→41.35 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 24.4256493167 Å / Origin y: 31.1975939398 Å / Origin z: 2.16098169233 Å
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Refinement TLS group | Selection details: chain 'A' and (resid 1 through 117 ) |