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Yorodumi- PDB-8qp0: A hexamer pore in the S-layer of Sulfolobus acidocaldarius formed... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8qp0 | ||||||
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Title | A hexamer pore in the S-layer of Sulfolobus acidocaldarius formed by SlaA protein | ||||||
Components | S-layer protein A | ||||||
Keywords | STRUCTURAL PROTEIN / S-layer / dimer / N-glycosylation / archea | ||||||
Function / homology | : / S-layer / extracellular region / S-layer protein A Function and homology information | ||||||
Biological species | Sulfolobus acidocaldarius DSM 639 (acidophilic) | ||||||
Method | ELECTRON MICROSCOPY / subtomogram averaging / cryo EM / Resolution: 11.2 Å | ||||||
Authors | Gambelli, L. / McLaren, M. / Isupov, M. / Conners, R. / Daum, B. | ||||||
Funding support | European Union, 1items
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Citation | Journal: Elife / Year: 2024 Title: Structure of the two-component S-layer of the archaeon . Authors: Lavinia Gambelli / Mathew McLaren / Rebecca Conners / Kelly Sanders / Matthew C Gaines / Lewis Clark / Vicki A M Gold / Daniel Kattnig / Mateusz Sikora / Cyril Hanus / Michail N Isupov / Bertram Daum / Abstract: Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell ...Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of . The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8qp0.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8qp0.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8qp0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8qp0_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 8qp0_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8qp0_validation.xml.gz | 203.9 KB | Display | |
Data in CIF | 8qp0_validation.cif.gz | 318.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qp/8qp0 ftp://data.pdbj.org/pub/pdb/validation_reports/qp/8qp0 | HTTPS FTP |
-Related structure data
Related structure data | 18127MC 7zcxC 8an2C 8an3C 8qoxC C: citing same article (ref.) M: map data used to model this data |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 151078.406 Da / Num. of mol.: 6 / Source method: isolated from a natural source Source: (natural) Sulfolobus acidocaldarius DSM 639 (acidophilic) References: UniProt: Q4J6E5 Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: 3D ARRAY / 3D reconstruction method: subtomogram averaging |
-Sample preparation
Component | Name: S-layer of Sulfolobus acidocaldarius / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic) |
Buffer solution | pH: 4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/2 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company | |||||||||||||||
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EM imaging | Cryogen: NITROGEN / Electron source: FIELD EMISSION GUN / Illumination mode: FLOOD BEAM / Mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 6000 nm / Nominal defocus min: 4000 nm / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Specimen-ID: 1
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Image recording |
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-Processing
EM software |
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Image processing | Details: Datasets were combined after particle-extraction in Relion then refined in M. | ||||||||||||||||||||||||||||||||||||||||||
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: C3 (3 fold cyclic) | ||||||||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 11.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 2271 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||||||||
EM volume selection | Num. of tomograms: 86 / Num. of volumes extracted: 22950 | ||||||||||||||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 7ZCX Accession code: 7ZCX / Source name: PDB / Type: experimental model | ||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
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