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Open data
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Basic information
Entry | Database: PDB / ID: 8qln | ||||||
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Title | Crystal structure of an N-terminal fragment of HCMV UL47 | ||||||
![]() | Inner tegument protein | ||||||
![]() | VIRAL PROTEIN / HCMV / tegument protein | ||||||
Function / homology | Herpesvirus tegument protein U30 / Herpes virus tegument protein U30 / Inner tegument protein / viral tegument / virion assembly / host cell Golgi apparatus / host cell nucleus / 6-tungstotellurate(VI) / Inner tegument protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rehfeld, C.L. / Reubold, T.F. / Eschenburg, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of an N-terminal fragment of HCMV UL47 Authors: Rehfeld, C.L. / Reubold, T.F. / Eschenburg, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.3 KB | Display | ![]() |
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PDB format | ![]() | 82.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 19.7 KB | Display | |
Data in CIF | ![]() | 25.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 62187.801 Da / Num. of mol.: 1 / Mutation: E161S, R162S, R163G, K165S, K166S, Q280S, K281S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-TEW / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.64 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 0.1 M Sodium acetate pH 4.6, 2 M sodium formate, 5 mM TEW |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 4, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978565 Å / Relative weight: 1 |
Reflection | Resolution: 2.51→46.46 Å / Num. obs: 20390 / % possible obs: 100 % / Redundancy: 25.3 % / Biso Wilson estimate: 68.61 Å2 / CC1/2: 0.999 / Rrim(I) all: 0.109 / Net I/σ(I): 18.35 |
Reflection shell | Resolution: 2.51→2.6 Å / Redundancy: 27 % / Num. unique obs: 1930 / CC1/2: 0.918 / Rrim(I) all: 1.043 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 73.21 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.51→46.44 Å
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Refine LS restraints |
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LS refinement shell |
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