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Open data
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Basic information
Entry | Database: PDB / ID: 8puz | ||||||
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Title | Crystal structure of tropomyosin (Cdc8) cables, Conformer 1 | ||||||
![]() | Tropomyosin | ||||||
![]() | STRUCTURAL PROTEIN / Tropomyosin / cdc8 / yeast / overlap complex | ||||||
Function / homology | ![]() plasma membrane fusion involved in cytogamy / formin-nucleated actin cable / mating projection actin fusion focus / RHOV GTPase cycle / actin lateral binding / actin cortical patch organization / mitotic actomyosin contractile ring assembly / mitotic actomyosin contractile ring / actomyosin contractile ring / actin cortical patch ...plasma membrane fusion involved in cytogamy / formin-nucleated actin cable / mating projection actin fusion focus / RHOV GTPase cycle / actin lateral binding / actin cortical patch organization / mitotic actomyosin contractile ring assembly / mitotic actomyosin contractile ring / actomyosin contractile ring / actin cortical patch / mitotic actomyosin contractile ring contraction / mating projection tip / cell division site / mitotic cytokinesis / actin filament / actin filament organization / actin filament binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Reinke, P.Y.A. / Zahn, M. / Fedorov, R. / Manstein, D.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Atomic structure of cdc8 tropomyosin cables Authors: Reinke, P.Y.A. / Manstein, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 171 KB | Display | ![]() |
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PDB format | ![]() | 114.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432.5 KB | Display | ![]() |
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Full document | ![]() | 435.4 KB | Display | |
Data in XML | ![]() | 12.6 KB | Display | |
Data in CIF | ![]() | 16.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 18995.045 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: cdc8, SPAC27F1.02c / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.84 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / Details: 1 M Tris HCl pH 8.2, and 45% MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 5, 2016 |
Radiation | Monochromator: 1 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→26.6 Å / Num. obs: 16997 / % possible obs: 99.9 % / Redundancy: 2.1 % / Biso Wilson estimate: 38.76 Å2 / CC1/2: 0.915 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 2.2→2.34 Å / Num. unique obs: 2706 / CC1/2: 0.915 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.08 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→24.63 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -0.000364267490094 Å / Origin y: -16.3960427191 Å / Origin z: 21.7802202118 Å
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Refinement TLS group | Selection details: all |