+Open data
-Basic information
Entry | Database: PDB / ID: 8prc | ||||||
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Title | The structure of v13Bagel8 | ||||||
Components | Cell surface protein | ||||||
Keywords | BIOSYNTHETIC PROTEIN / Protein design / symmetric / assembly / self-assembly / beta-propeller | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Dictyoglomus thermophilum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||
Authors | Vandebroek, L. / Voet, A.R.D. / Lee, X.Y. | ||||||
Funding support | Belgium, 1items
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Citation | Journal: To Be Published Title: The structure of v13Bagel8 Authors: Vandebroek, L. / Voet, A.R.D. / Lee, X.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8prc.cif.gz | 30.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8prc.ent.gz | 17.9 KB | Display | PDB format |
PDBx/mmJSON format | 8prc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8prc_validation.pdf.gz | 1.6 MB | Display | wwPDB validaton report |
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Full document | 8prc_full_validation.pdf.gz | 1.6 MB | Display | |
Data in XML | 8prc_validation.xml.gz | 5.1 KB | Display | |
Data in CIF | 8prc_validation.cif.gz | 6.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pr/8prc ftp://data.pdbj.org/pub/pdb/validation_reports/pr/8prc | HTTPS FTP |
-Related structure data
Related structure data | 8prjC 8prlC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 9107.999 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Dictyoglomus thermophilum (bacteria) / Strain: ATCC 35947 / DSM 3960 / H-6-12 / Gene: DICTH_0179 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: B5YBJ6 | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.21 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion / pH: 6.5 Details: 0.16 M Zinc acetate, 0.08 M Sodium cacodylate pH 6.5, 14.4 %(w/v) PEG 8000, 20 %(v/v) Glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.976292 Å |
Detector | Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Jul 30, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976292 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→48.55 Å / Num. obs: 6417 / % possible obs: 100 % / Redundancy: 25.4 % / CC1/2: 0.999 / Rmerge(I) obs: 0.135 / Rpim(I) all: 0.028 / Rrim(I) all: 0.138 / Χ2: 1.02 / Net I/σ(I): 19.6 |
Reflection shell | Resolution: 2.35→2.43 Å / % possible obs: 100 % / Redundancy: 26.8 % / Rmerge(I) obs: 0.634 / Num. measured all: 9240 / Num. unique obs: 345 / CC1/2: 0.994 / Rpim(I) all: 0.124 / Rrim(I) all: 0.646 / Χ2: 0.99 / Net I/σ(I) obs: 4.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.35→38.3 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 42.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→38.3 Å
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Refine LS restraints |
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LS refinement shell |
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