Evidence: gel filtration, There is one molecule of the Gel4-Nb3 complex by gel filtration.
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
4430 Å2
ΔGint
16 kcal/mol
Surface area
23520 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
96.992, 96.992, 106.625
Angle α, β, γ (deg.)
90.00, 90.00, 120.00
Int Tables number
152
Space group name H-M
P3121
-
Components
-
Protein / Antibody , 2 types, 2 molecules AB
#1: Protein
1,3-beta-glucanosyltransferase
Mass: 49737.863 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aspergillus fumigatus (mold) / Gene: CDV57_08969 / Production host: Komagataella pastoris (fungus) / References: UniProt: A0A229W401
#2: Antibody
Nanobody3
Mass: 13290.772 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
Resolution: 2.05→19.54 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.952 / SU B: 2.622 / SU ML: 0.075 / Cross valid method: THROUGHOUT / ESU R: 0.04 / ESU R Free: 0.033 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20603
1514
4.1 %
RANDOM
Rwork
0.17233
-
-
-
obs
0.17369
35285
99.77 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK