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Open data
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Basic information
Entry | Database: PDB / ID: 8paz | |||||||||
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Title | OXIDIZED NATIVE PSEUDOAZURIN FROM A. FAECALIS | |||||||||
![]() | PSEUDOAZURIN | |||||||||
![]() | ELECTRON TRANSFER / CUPROPROTEIN | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Adman, E.T. / Libeu, C.A.P. | |||||||||
![]() | ![]() Title: Site-directed mutants of pseudoazurin: explanation of increased redox potentials from X-ray structures and from calculation of redox potential differences. Authors: Libeu, C.A. / Kukimoto, M. / Nishiyama, M. / Horinouchi, S. / Adman, E.T. #1: ![]() Title: Site-Directed Mutagenesis of Pseudoazurin from Alcaligenes Faecalis S-6; Pro80Ala Mutant Exhibits Marked Increase in Reduction Potential Authors: Nishiyama, M. / Suzuki, J. / Ohnuki, T. / Chang, H.C. / Horinouchi, S. / Turley, S. / Adman, E.T. / Beppu, T. #2: ![]() Title: A 2.0-A Structure of the Blue Copper Protein (Cupredoxin) from Alcaligenes Faecalis S-6 Authors: Adman, E.T. / Turley, S. / Bramson, R. / Petratos, K. / Banner, D. / Tsernoglou, D. / Beppu, T. / Watanabe, H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.3 KB | Display | ![]() |
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PDB format | ![]() | 26 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 412.3 KB | Display | ![]() |
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Full document | ![]() | 413.4 KB | Display | |
Data in XML | ![]() | 7.7 KB | Display | |
Data in CIF | ![]() | 9.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13383.511 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.87 % | ||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 50MM SODIUM PHOSPHATE WITH 75% SATURATED AMMONIUM SULFATE., pH 6.5 | ||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Apr 1, 1992 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→26.2 Å / Num. obs: 18528 / Observed criterion σ(I): 0 / Redundancy: 5 % / Rmerge(I) obs: 0.053 / Net I/σ(I): 20.6 |
Reflection shell | Resolution: 1.6→1.7 Å / Rmerge(I) obs: 0.15 / Mean I/σ(I) obs: 5.8 |
Reflection | *PLUS % possible obs: 88 % / Rmerge(I) obs: 0.044 |
Reflection shell | *PLUS % possible obs: 65 % |
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Processing
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Refinement | Method to determine structure: KNOWN / Resolution: 1.6→26.2 Å / Num. parameters: 4205 / Num. restraintsaints: 4058 / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER Details: COPPER-LIGAND DISTANCES NOT RESTRAINED; CU + S REFINED ANISOTROPICALLY.
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Solvent computation | Solvent model: BABINET'S PRINCIPLE (SHELX-93) | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 10 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→26.2 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.167 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |