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Open data
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Basic information
Entry | Database: PDB / ID: 8p2b | ||||||
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Title | Crystal structure of CbFMN4 domain 1 | ||||||
![]() | Clostridiaceae bacterium FMN4 domain 1 | ||||||
![]() | FLAVOPROTEIN / multi-flavinylated protein / FMN / post-translational | ||||||
Function / homology | FLAVIN MONONUCLEOTIDE![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rozeboom, H.J. / Fraaije, M.W. | ||||||
Funding support | 1items
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![]() | ![]() Title: Characterization of two bacterial multi-flavinylated proteins harboring multiple covalent flavin cofactors. Authors: Tong, Y. / Rozeboom, H.J. / Loonstra, M.R. / Wijma, H.J. / Fraaije, M.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.2 KB | Display | ![]() |
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PDB format | ![]() | 59.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 987.3 KB | Display | ![]() |
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Full document | ![]() | 987.6 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8p2aC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9061.036 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 63 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7.7 / Details: 3.4 M ammonium sulfate, 0.1 M bis-tris-propane |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Aug 24, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9655 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→40.9 Å / Num. obs: 8547 / % possible obs: 100 % / Redundancy: 9.7 % / CC1/2: 0.998 / Rmerge(I) obs: 0.152 / Rpim(I) all: 0.052 / Rrim(I) all: 0.161 / Χ2: 1.03 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.6→2.72 Å / % possible obs: 100 % / Redundancy: 9.5 % / Rmerge(I) obs: 2.623 / Num. measured all: 10011 / Num. unique obs: 1052 / CC1/2: 0.526 / Rpim(I) all: 0.894 / Rrim(I) all: 2.773 / Χ2: 0.88 / Net I/σ(I) obs: 0.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.6 Å2
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Refinement step | Cycle: 1 / Resolution: 2.6→40.9 Å
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Refine LS restraints |
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