+Open data
-Basic information
Entry | Database: PDB / ID: 8p1u | ||||||
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Title | Structure of divisome complex FtsWIQLB | ||||||
Components |
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Keywords | CELL CYCLE / FtsWIQLB / Gram-negative bacteria / membrane protein / divisome | ||||||
Function / homology | Function and homology information cell septum assembly / lipid-linked peptidoglycan transporter activity / peptidoglycan glycosyltransferase / peptidoglycan glycosyltransferase activity / cell septum / serine-type D-Ala-D-Ala carboxypeptidase / FtsZ-dependent cytokinesis / serine-type D-Ala-D-Ala carboxypeptidase activity / division septum assembly / cell division site ...cell septum assembly / lipid-linked peptidoglycan transporter activity / peptidoglycan glycosyltransferase / peptidoglycan glycosyltransferase activity / cell septum / serine-type D-Ala-D-Ala carboxypeptidase / FtsZ-dependent cytokinesis / serine-type D-Ala-D-Ala carboxypeptidase activity / division septum assembly / cell division site / penicillin binding / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / cell division / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.3 Å | ||||||
Authors | Yang, L. / Chang, S. / Tang, D. / Dong, H. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell Discov / Year: 2024 Title: Structural insights into the activation of the divisome complex FtsWIQLB. Authors: Lili Yang / Yujiao Chen / Shenghai Chang / Chongrong Shen / Xin Wang / Changbin Zhang / Zhibo Zhang / Bi-Sen Ding / Zhaoming Su / Haohao Dong / Xiaodi Tang / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8p1u.cif.gz | 217.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8p1u.ent.gz | 169.5 KB | Display | PDB format |
PDBx/mmJSON format | 8p1u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8p1u_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 8p1u_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 8p1u_validation.xml.gz | 47.7 KB | Display | |
Data in CIF | 8p1u_validation.cif.gz | 71 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p1/8p1u ftp://data.pdbj.org/pub/pdb/validation_reports/p1/8p1u | HTTPS FTP |
-Related structure data
Related structure data | 17356MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 11150.034 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: ftsL, PA4419 / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q9HVZ6 |
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#2: Protein | Mass: 43793.629 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: ftsW, PA4413 / Production host: Escherichia coli K-12 (bacteria) References: UniProt: Q9HW00, peptidoglycan glycosyltransferase |
#3: Protein | Mass: 62933.082 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: ftsI, pbpB, PA4418 / Production host: Escherichia coli K-12 (bacteria) References: UniProt: G3XD46, serine-type D-Ala-D-Ala carboxypeptidase |
#4: Protein | Mass: 10890.521 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: ftsB, PA3634 / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q9HXZ6 |
#5: Protein | Mass: 32290.223 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: ftsQ, PA4409 / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: G3XDA7 |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: divisome complex FtsWIQLB / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Escherichia coli K-12 (bacteria) |
Source (recombinant) | Organism: Escherichia coli K-12 (bacteria) |
Buffer solution | pH: 7.8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Calibrated defocus min: 1000 nm / Calibrated defocus max: 2000 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.20.1_4487: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 340758 / Symmetry type: POINT | ||||||||||||||||||||||||
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