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Open data
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Basic information
Entry | Database: PDB / ID: 8osp | ||||||
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Title | GCN5-related N-Acetyltransferase from Lactobacillus curiae | ||||||
![]() | N-acetyltransferase | ||||||
![]() | TRANSFERASE / GNAT / L curiae / canavanine acetylation / CoA | ||||||
Function / homology | acyltransferase activity, transferring groups other than amino-acyl groups / Acetyltransferase (GNAT) family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / GNAT domain / Acyl-CoA N-acyltransferase / PHOSPHATE ION / N-acetyltransferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fleming, J.R. / Mayans, O. | ||||||
Funding support | 1items
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![]() | ![]() Title: Crystal structure of a GCN5-related N-acetyltransferase from Lactobacillus curiae. Authors: Fleming, J.R. / Hauth, F. / Hartig, J.S. / Mayans, O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 269.6 KB | Display | ![]() |
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PDB format | ![]() | 182.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 469.2 KB | Display | ![]() |
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Full document | ![]() | 474.1 KB | Display | |
Data in XML | ![]() | 17.9 KB | Display | |
Data in CIF | ![]() | 24.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20280.246 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-MES / #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.51 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion, sitting drop Details: 1 M Potassium sodium tartrate, 0.1 M MES sodium hydroxide pH 6.0 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 21, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.000021 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→41.45 Å / Num. obs: 26388 / % possible obs: 96.38 % / Redundancy: 11.54 % / Biso Wilson estimate: 29.54 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.15 / Rrim(I) all: 0.1627 / Net I/σ(I): 10.07 |
Reflection shell | Resolution: 1.95→2.02 Å / Rmerge(I) obs: 0.9604 / Mean I/σ(I) obs: 2.02 / Num. unique obs: 2286 / CC1/2: 0.77 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.39 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→41.45 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION / Label seq-ID: 1
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